Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 1.83 MB
Add to list
  1. Search results
  2. Intracellular delivery of mRNA in adh...

Intracellular delivery of mRNA in adherent and suspension cells by vapor nanobubble photoporation

Laurens Raes, Stephan Stremersch (UGent) , Juan Fraire (UGent) , Toon Brans (UGent) , Glenn Goetgeluk (UGent) , Stijn De Munter (UGent) , Lien Van Hoecke (UGent) , Rein Verbeke (UGent) , Jelter Van Hoeck, Ranhua Xiong (UGent) , et al.
(2020) NANO-MICRO LETTERS. 12(1).
Author
Organization
Abstract
Efficient and safe cell engineering by transfection of nucleic acids remains one of the long-standing hurdles for fundamental biomedical research and many new therapeutic applications, such as CAR T cell-based therapies. mRNA has recently gained increasing attention as a more safe and versatile alternative tool over viral- or DNA transposon-based approaches for the generation of adoptive T cells. However, limitations associated with existing nonviral mRNA delivery approaches hamper progress on genetic engineering of these hard-to-transfect immune cells. In this study, we demonstrate that gold nanoparticle-mediated vapor nanobubble (VNB) photoporation is a promising upcoming physical transfection method capable of delivering mRNA in both adherent and suspension cells. Initial transfection experiments on HeLa cells showed the importance of transfection buffer and cargo concentration, while the technology was furthermore shown to be effective for mRNA delivery in Jurkat T cells with transfection efficiencies up to 45%. Importantly, compared to electroporation, which is the reference technology for nonviral transfection of T cells, a fivefold increase in the number of transfected viable Jurkat T cells was observed. Altogether, our results point toward the use of VNB photoporation as a more gentle and efficient technology for intracellular mRNA delivery in adherent and suspension cells, with promising potential for the future engineering of cells in therapeutic and fundamental research applications.
Keywords
Electrical and Electronic Engineering, Electronic, Optical and Magnetic Materials, Surfaces, Coatings and Films, Transfection, mRNA, Photoporation, Optoporation, Gold nanoparticles, Vapor nanobubbles, T-CELLS, GOLD NANOPARTICLES, IN-VITRO, RECEPTOR, NUCLEOFECTION, IMMUNOTHERAPY, TRANSFECTION, INJECTIONS, INNATE, GUIDE

Downloads

  • Download
    Raes2020 Article IntracellularDeliveryOfMRNAInA.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 1.83 MB

Citation

Please use this url to cite or link to this publication:

MLA
Raes, Laurens, et al. “Intracellular Delivery of MRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation.” NANO-MICRO LETTERS, vol. 12, no. 1, 2020, doi:10.1007/s40820-020-00523-0.
APA
Raes, L., Stremersch, S., Fraire, J., Brans, T., Goetgeluk, G., De Munter, S., … Braeckmans, K. (2020). Intracellular delivery of mRNA in adherent and suspension cells by vapor nanobubble photoporation. NANO-MICRO LETTERS, 12(1). https://doi.org/10.1007/s40820-020-00523-0
Chicago author-date
Raes, Laurens, Stephan Stremersch, Juan Fraire, Toon Brans, Glenn Goetgeluk, Stijn De Munter, Lien Van Hoecke, et al. 2020. “Intracellular Delivery of MRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation.” NANO-MICRO LETTERS 12 (1). https://doi.org/10.1007/s40820-020-00523-0.
Chicago author-date (all authors)
Raes, Laurens, Stephan Stremersch, Juan Fraire, Toon Brans, Glenn Goetgeluk, Stijn De Munter, Lien Van Hoecke, Rein Verbeke, Jelter Van Hoeck, Ranhua Xiong, Xavier Saelens, Bart Vandekerckhove, Stefaan De Smedt, Koen Raemdonck, and Kevin Braeckmans. 2020. “Intracellular Delivery of MRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation.” NANO-MICRO LETTERS 12 (1). doi:10.1007/s40820-020-00523-0.
Vancouver
1.
Raes L, Stremersch S, Fraire J, Brans T, Goetgeluk G, De Munter S, et al. Intracellular delivery of mRNA in adherent and suspension cells by vapor nanobubble photoporation. NANO-MICRO LETTERS. 2020;12(1).
IEEE
[1]
L. Raes et al., “Intracellular delivery of mRNA in adherent and suspension cells by vapor nanobubble photoporation,” NANO-MICRO LETTERS, vol. 12, no. 1, 2020.
@article{8677305,
  abstract     = {{Efficient and safe cell engineering by transfection of nucleic acids remains one of the long-standing hurdles for fundamental biomedical research and many new therapeutic applications, such as CAR T cell-based therapies. mRNA has recently gained increasing attention as a more safe and versatile alternative tool over viral- or DNA transposon-based approaches for the generation of adoptive T cells. However, limitations associated with existing nonviral mRNA delivery approaches hamper progress on genetic engineering of these hard-to-transfect immune cells. In this study, we demonstrate that gold nanoparticle-mediated vapor nanobubble (VNB) photoporation is a promising upcoming physical transfection method capable of delivering mRNA in both adherent and suspension cells. Initial transfection experiments on HeLa cells showed the importance of transfection buffer and cargo concentration, while the technology was furthermore shown to be effective for mRNA delivery in Jurkat T cells with transfection efficiencies up to 45%. Importantly, compared to electroporation, which is the reference technology for nonviral transfection of T cells, a fivefold increase in the number of transfected viable Jurkat T cells was observed. Altogether, our results point toward the use of VNB photoporation as a more gentle and efficient technology for intracellular mRNA delivery in adherent and suspension cells, with promising potential for the future engineering of cells in therapeutic and fundamental research applications.}},
  articleno    = {{185}},
  author       = {{Raes, Laurens and Stremersch, Stephan and Fraire, Juan and Brans, Toon and Goetgeluk, Glenn and De Munter, Stijn and Van Hoecke, Lien and Verbeke, Rein and Van Hoeck, Jelter and Xiong, Ranhua and Saelens, Xavier and Vandekerckhove, Bart and De Smedt, Stefaan and Raemdonck, Koen and Braeckmans, Kevin}},
  issn         = {{2311-6706}},
  journal      = {{NANO-MICRO LETTERS}},
  keywords     = {{Electrical and Electronic Engineering,Electronic,Optical and Magnetic Materials,Surfaces,Coatings and Films,Transfection,mRNA,Photoporation,Optoporation,Gold nanoparticles,Vapor nanobubbles,T-CELLS,GOLD NANOPARTICLES,IN-VITRO,RECEPTOR,NUCLEOFECTION,IMMUNOTHERAPY,TRANSFECTION,INJECTIONS,INNATE,GUIDE}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{17}},
  title        = {{Intracellular delivery of mRNA in adherent and suspension cells by vapor nanobubble photoporation}},
  url          = {{http://doi.org/10.1007/s40820-020-00523-0}},
  volume       = {{12}},
  year         = {{2020}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: