Advanced search
1 file | 889.54 KB Add to list

The impact of therapeutic-dose induced intestinal enrofloxacin concentrations in healthy pigs on fecal Escherichia coli populations

Joren De Smet (UGent) , Filip Boyen (UGent) , Siska Croubels (UGent) , Geertrui Rasschaert, Freddy Haesebrouck (UGent) , Robin Temmerman (UGent) , Sofie Rutjens (UGent) , Patrick De Backer (UGent) and Mathias Devreese (UGent)
Author
Organization
Abstract
Background: Knowledge of therapy-induced intestinal tract concentrations of antimicrobials allows for interpretation and prediction of antimicrobial resistance selection within the intestinal microbiota. This study describes the impact of three different doses of enrofloxacin (ENR) and two different administration routes on the intestinal concentration of ENR and on the fecal Escherichia coli populations in pigs. Enrofloxacin was administered on three consecutive days to four different treatment groups. The groups either received an oral bolus administration of ENR (conventional or half dose) or an intramuscular administration (conventional or double dose). Results: Quantitative analysis of fecal samples showed high ENR concentrations in all groups, ranging from 5.114 ± 1.272 μg/g up to 39.54 ± 10.43 μg/g at the end of the treatment period. In addition, analysis of the luminal intestinal content revealed an increase of ENR concentration from the proximal to the distal intestinal tract segments, with no significant effect of administration route. Fecal samples were also screened for resistance in E. coli isolates against ENR. Wild-type (MIC≤0.125 μg/mL) and non-wild-type (0.125 < MIC≤2 μg/mL) E. coli isolates were found at time 0 h. At the end of treatment (3 days) only non-wild-type isolates (MIC≥32 μg/mL) were found. Conclusions: In conclusion, the observed intestinal ENR concentrations in all groups showed to be both theoretically (based on pharmacokinetic and pharmacodynamic principles) and effectively (in vivo measurement) capable of significantly reducing the intestinal E. coli wild-type population.
Keywords
Escherichia coli, Microbiota, Enrofloxacin, Antimicrobials, Antimicrobial resistance, Dose, Administration route, ANTIBIOTIC-RESISTANCE, FLUOROQUINOLONE RESISTANCE, ANTIMICROBIAL RESISTANCE, LIQUID-CHROMATOGRAPHY, QUINOLONE RESISTANCE, ADMINISTRATION ROUTE, CIPROFLOXACIN, SELECTION, GENES, MECHANISMS

Downloads

  • Smet et al-2020-BMC Veterinary Research.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 889.54 KB

Citation

Please use this url to cite or link to this publication:

MLA
De Smet, Joren, et al. “The Impact of Therapeutic-Dose Induced Intestinal Enrofloxacin Concentrations in Healthy Pigs on Fecal Escherichia Coli Populations.” BMC VETERINARY RESEARCH, vol. 16, 2020, doi:10.1186/s12917-020-02608-9.
APA
De Smet, J., Boyen, F., Croubels, S., Rasschaert, G., Haesebrouck, F., Temmerman, R., … Devreese, M. (2020). The impact of therapeutic-dose induced intestinal enrofloxacin concentrations in healthy pigs on fecal Escherichia coli populations. BMC VETERINARY RESEARCH, 16. https://doi.org/10.1186/s12917-020-02608-9
Chicago author-date
De Smet, Joren, Filip Boyen, Siska Croubels, Geertrui Rasschaert, Freddy Haesebrouck, Robin Temmerman, Sofie Rutjens, Patrick De Backer, and Mathias Devreese. 2020. “The Impact of Therapeutic-Dose Induced Intestinal Enrofloxacin Concentrations in Healthy Pigs on Fecal Escherichia Coli Populations.” BMC VETERINARY RESEARCH 16. https://doi.org/10.1186/s12917-020-02608-9.
Chicago author-date (all authors)
De Smet, Joren, Filip Boyen, Siska Croubels, Geertrui Rasschaert, Freddy Haesebrouck, Robin Temmerman, Sofie Rutjens, Patrick De Backer, and Mathias Devreese. 2020. “The Impact of Therapeutic-Dose Induced Intestinal Enrofloxacin Concentrations in Healthy Pigs on Fecal Escherichia Coli Populations.” BMC VETERINARY RESEARCH 16. doi:10.1186/s12917-020-02608-9.
Vancouver
1.
De Smet J, Boyen F, Croubels S, Rasschaert G, Haesebrouck F, Temmerman R, et al. The impact of therapeutic-dose induced intestinal enrofloxacin concentrations in healthy pigs on fecal Escherichia coli populations. BMC VETERINARY RESEARCH. 2020;16.
IEEE
[1]
J. De Smet et al., “The impact of therapeutic-dose induced intestinal enrofloxacin concentrations in healthy pigs on fecal Escherichia coli populations,” BMC VETERINARY RESEARCH, vol. 16, 2020.
@article{8677303,
  abstract     = {Background: Knowledge of therapy-induced intestinal tract concentrations of antimicrobials allows for interpretation and prediction of antimicrobial resistance selection within the intestinal microbiota. This study describes the impact of three different doses of enrofloxacin (ENR) and two different administration routes on the intestinal concentration of ENR and on the fecal Escherichia coli populations in pigs. Enrofloxacin was administered on three consecutive days to four different treatment groups. The groups either received an oral bolus administration of ENR (conventional or half dose) or an intramuscular administration (conventional or double dose).

Results: Quantitative analysis of fecal samples showed high ENR concentrations in all groups, ranging from 5.114 ± 1.272 μg/g up to 39.54 ± 10.43 μg/g at the end of the treatment period. In addition, analysis of the luminal intestinal content revealed an increase of ENR concentration from the proximal to the distal intestinal tract segments, with no significant effect of administration route. Fecal samples were also screened for resistance in E. coli isolates against ENR. Wild-type (MIC≤0.125 μg/mL) and non-wild-type (0.125 < MIC≤2 μg/mL) E. coli isolates were found at time 0 h. At the end of treatment (3 days) only non-wild-type isolates (MIC≥32 μg/mL) were found.

Conclusions: In conclusion, the observed intestinal ENR concentrations in all groups showed to be both theoretically (based on pharmacokinetic and pharmacodynamic principles) and effectively (in vivo measurement) capable of significantly reducing the intestinal E. coli wild-type population.},
  articleno    = {382},
  author       = {De Smet, Joren and Boyen, Filip and Croubels, Siska and Rasschaert, Geertrui and Haesebrouck, Freddy and Temmerman, Robin and Rutjens, Sofie and De Backer, Patrick and Devreese, Mathias},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keywords     = {Escherichia coli,Microbiota,Enrofloxacin,Antimicrobials,Antimicrobial resistance,Dose,Administration route,ANTIBIOTIC-RESISTANCE,FLUOROQUINOLONE RESISTANCE,ANTIMICROBIAL RESISTANCE,LIQUID-CHROMATOGRAPHY,QUINOLONE RESISTANCE,ADMINISTRATION ROUTE,CIPROFLOXACIN,SELECTION,GENES,MECHANISMS},
  language     = {eng},
  pages        = {12},
  title        = {The impact of therapeutic-dose induced intestinal enrofloxacin concentrations in healthy pigs on fecal Escherichia coli populations},
  url          = {http://dx.doi.org/10.1186/s12917-020-02608-9},
  volume       = {16},
  year         = {2020},
}

Altmetric
View in Altmetric
Web of Science
Times cited: