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Dissecting cholesterol and phytosterol biosynthesis via mutants and inhibitors

Kjell De Vriese, Jacob Pollier (UGent) , Alain Goossens (UGent) , Tom Beeckman (UGent) and Steffen Vanneste (UGent)
(2021) JOURNAL OF EXPERIMENTAL BOTANY. 72(2). p.241-253
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Abstract
Plants stand out among eukaryotes due to the large variety of sterols and sterol derivatives that they can produce. These metabolites not only serve as critical determinants of membrane structures, but also act as signaling molecules, as growth-regulating hormones, or as modulators of enzyme activities. Therefore, it is critical to understand the wiring of the biosynthetic pathways by which plants generate these distinct sterols, to allow their manipulation and to dissect their precise physiological roles. Here, we review the complexity and variation of the biosynthetic routes of the most abundant phytosterols and cholesterol in the green lineage and how different enzymes in these pathways are conserved and diverged from humans, yeast, and even bacteria. Many enzymatic steps show a deep evolutionary conservation, while others are executed by completely different enzymes. This has important implications for the use and specificity of available human and yeast sterol biosynthesis inhibitors in plants, and argues for the development of plant-tailored inhibitors of sterol biosynthesis.
Keywords
Plant Science, Physiology, Campesterol, cholesterol, inhibitor, mutant, sitosterol, stigmasterol, STEROL O-ACYLTRANSFERASE, ARABIDOPSIS-THALIANA, ISOPRENOID BIOSYNTHESIS, SQUALENE SYNTHASE, BRASSINOSTEROID BIOSYNTHESIS, FARNESYL DIPHOSPHATE, LANOSTEROL SYNTHASE, GENE ENCODES, PLANT, PATHWAYS

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Citation

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MLA
De Vriese, Kjell, et al. “Dissecting Cholesterol and Phytosterol Biosynthesis via Mutants and Inhibitors.” JOURNAL OF EXPERIMENTAL BOTANY, vol. 72, no. 2, 2021, pp. 241–53, doi:10.1093/jxb/eraa429.
APA
De Vriese, K., Pollier, J., Goossens, A., Beeckman, T., & Vanneste, S. (2021). Dissecting cholesterol and phytosterol biosynthesis via mutants and inhibitors. JOURNAL OF EXPERIMENTAL BOTANY, 72(2), 241–253. https://doi.org/10.1093/jxb/eraa429
Chicago author-date
De Vriese, Kjell, Jacob Pollier, Alain Goossens, Tom Beeckman, and Steffen Vanneste. 2021. “Dissecting Cholesterol and Phytosterol Biosynthesis via Mutants and Inhibitors.” JOURNAL OF EXPERIMENTAL BOTANY 72 (2): 241–53. https://doi.org/10.1093/jxb/eraa429.
Chicago author-date (all authors)
De Vriese, Kjell, Jacob Pollier, Alain Goossens, Tom Beeckman, and Steffen Vanneste. 2021. “Dissecting Cholesterol and Phytosterol Biosynthesis via Mutants and Inhibitors.” JOURNAL OF EXPERIMENTAL BOTANY 72 (2): 241–253. doi:10.1093/jxb/eraa429.
Vancouver
1.
De Vriese K, Pollier J, Goossens A, Beeckman T, Vanneste S. Dissecting cholesterol and phytosterol biosynthesis via mutants and inhibitors. JOURNAL OF EXPERIMENTAL BOTANY. 2021;72(2):241–53.
IEEE
[1]
K. De Vriese, J. Pollier, A. Goossens, T. Beeckman, and S. Vanneste, “Dissecting cholesterol and phytosterol biosynthesis via mutants and inhibitors,” JOURNAL OF EXPERIMENTAL BOTANY, vol. 72, no. 2, pp. 241–253, 2021.
@article{8676892,
  abstract     = {{Plants stand out among eukaryotes due to the large variety of sterols and sterol derivatives that they can produce. These metabolites not only serve as critical determinants of membrane structures, but also act as signaling molecules, as growth-regulating hormones, or as modulators of enzyme activities. Therefore, it is critical to understand the wiring of the biosynthetic pathways by which plants generate these distinct sterols, to allow their manipulation and to dissect their precise physiological roles. Here, we review the complexity and variation of the biosynthetic routes of the most abundant phytosterols and cholesterol in the green lineage and how different enzymes in these pathways are conserved and diverged from humans, yeast, and even bacteria. Many enzymatic steps show a deep evolutionary conservation, while others are executed by completely different enzymes. This has important implications for the use and specificity of available human and yeast sterol biosynthesis inhibitors in plants, and argues for the development of plant-tailored inhibitors of sterol biosynthesis.}},
  author       = {{De Vriese, Kjell and Pollier, Jacob and Goossens, Alain and Beeckman, Tom and Vanneste, Steffen}},
  issn         = {{0022-0957}},
  journal      = {{JOURNAL OF EXPERIMENTAL BOTANY}},
  keywords     = {{Plant Science,Physiology,Campesterol,cholesterol,inhibitor,mutant,sitosterol,stigmasterol,STEROL O-ACYLTRANSFERASE,ARABIDOPSIS-THALIANA,ISOPRENOID BIOSYNTHESIS,SQUALENE SYNTHASE,BRASSINOSTEROID BIOSYNTHESIS,FARNESYL DIPHOSPHATE,LANOSTEROL SYNTHASE,GENE ENCODES,PLANT,PATHWAYS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{241--253}},
  title        = {{Dissecting cholesterol and phytosterol biosynthesis via mutants and inhibitors}},
  url          = {{http://dx.doi.org/10.1093/jxb/eraa429}},
  volume       = {{72}},
  year         = {{2021}},
}

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