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Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele

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Abstract
Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(−/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding protein. Despite having 10% less lignin, CCR2(−/*) line 12 grows normally. On a plant basis, the saccharification efficiency of CCR2(−/*) line 12 is increased by 25–41%, depending on the pretreatment. Analysis of monoallelic CCR2 knockout lines shows that the reduced lignin amount in CCR2(−/*) line 12 is due to the combination of a null and the specific haploinsufficient CCR2 allele. Analysis of another CCR2(−/*) line shows that depending on the specific CCR2 amino-acid change, lignin amount and growth can be affected to different extents. Our findings open up new possibilities for stably fine-tuning residual gene function in planta.
Keywords
General Biochemistry, Genetics and Molecular Biology, General Physics and Astronomy, General Chemistry, CINNAMOYL-COA REDUCTASE, DOWN-REGULATION, ALCOHOL-DEHYDROGENASE, SACCHARIFICATION, LIGNIFICATION, METABOLISM, EXPRESSION, DEFICIENT, REVEALS, PATHWAY

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MLA
De Meester, Barbara, et al. “Tailoring Poplar Lignin without Yield Penalty by Combining a Null and Haploinsufficient CINNAMOYL-CoA REDUCTASE2 Allele.” NATURE COMMUNICATIONS, vol. 11, no. 1, 2020, doi:10.1038/s41467-020-18822-w.
APA
De Meester, B., Madariaga Calderón, B. L., de Vries, L., Pollier, J., Goeminne, G., Van Doorsselaere, J., … Boerjan, W. (2020). Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele. NATURE COMMUNICATIONS, 11(1). https://doi.org/10.1038/s41467-020-18822-w
Chicago author-date
De Meester, Barbara, Barbara Loreto Madariaga Calderón, Lisanne de Vries, Jacob Pollier, Geert Goeminne, Jan Van Doorsselaere, Mingjie Chen, John Ralph, Ruben Vanholme, and Wout Boerjan. 2020. “Tailoring Poplar Lignin without Yield Penalty by Combining a Null and Haploinsufficient CINNAMOYL-CoA REDUCTASE2 Allele.” NATURE COMMUNICATIONS 11 (1). https://doi.org/10.1038/s41467-020-18822-w.
Chicago author-date (all authors)
De Meester, Barbara, Barbara Loreto Madariaga Calderón, Lisanne de Vries, Jacob Pollier, Geert Goeminne, Jan Van Doorsselaere, Mingjie Chen, John Ralph, Ruben Vanholme, and Wout Boerjan. 2020. “Tailoring Poplar Lignin without Yield Penalty by Combining a Null and Haploinsufficient CINNAMOYL-CoA REDUCTASE2 Allele.” NATURE COMMUNICATIONS 11 (1). doi:10.1038/s41467-020-18822-w.
Vancouver
1.
De Meester B, Madariaga Calderón BL, de Vries L, Pollier J, Goeminne G, Van Doorsselaere J, et al. Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele. NATURE COMMUNICATIONS. 2020;11(1).
IEEE
[1]
B. De Meester et al., “Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele,” NATURE COMMUNICATIONS, vol. 11, no. 1, 2020.
@article{8676875,
  abstract     = {Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(−/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding protein. Despite having 10% less lignin, CCR2(−/*) line 12 grows normally. On a plant basis, the saccharification efficiency of CCR2(−/*) line 12 is increased by 25–41%, depending on the pretreatment. Analysis of monoallelic CCR2 knockout lines shows that the reduced lignin amount in CCR2(−/*) line 12 is due to the combination of a null and the specific haploinsufficient CCR2 allele. Analysis of another CCR2(−/*) line shows that depending on the specific CCR2 amino-acid change, lignin amount and growth can be affected to different extents. Our findings open up new possibilities for stably fine-tuning residual gene function in planta.},
  articleno    = {5020},
  author       = {De Meester, Barbara and Madariaga Calderón, Barbara Loreto and de Vries, Lisanne and Pollier, Jacob and Goeminne, Geert and Van Doorsselaere, Jan and Chen, Mingjie and Ralph, John and Vanholme, Ruben and Boerjan, Wout},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keywords     = {General Biochemistry,Genetics and Molecular Biology,General Physics and Astronomy,General Chemistry,CINNAMOYL-COA REDUCTASE,DOWN-REGULATION,ALCOHOL-DEHYDROGENASE,SACCHARIFICATION,LIGNIFICATION,METABOLISM,EXPRESSION,DEFICIENT,REVEALS,PATHWAY},
  language     = {eng},
  number       = {1},
  pages        = {13},
  title        = {Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele},
  url          = {http://dx.doi.org/10.1038/s41467-020-18822-w},
  volume       = {11},
  year         = {2020},
}

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