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Keeping in touch with type-III secretion system effectors : mass spectrometry-based proteomics to study effector-host protein-protein interactions

Margaux De Meyer (UGent) , Joren De Ryck (UGent) , Sofie Goormachtig (UGent) and Petra Van Damme (UGent)
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  • PROPHECY (PROPHECY: Translational control in infection biology: riboproteogenomics of bacterial pathogens)
Abstract
Manipulation of host cellular processes by translocated bacterial effectors is key to the success of bacterial pathogens and some symbionts. Therefore, a comprehensive understanding of effectors is of critical importance to understand infection biology. It has become increasingly clear that the identification of host protein targets contributes invaluable knowledge to the characterization of effector function during pathogenesis. Recent advances in mapping protein–protein interaction networks by means of mass spectrometry-based interactomics have enabled the identification of host targets at large-scale. In this review, we highlight mass spectrometry-driven proteomics strategies and recent advances to elucidate type-III secretion system effector–host protein–protein interactions. Furthermore, we highlight approaches for defining spatial and temporal effector–host interactions, and discuss possible avenues for studying natively delivered effectors in the context of infection. Overall, the knowledge gained when unravelling effector complexation with host factors will provide novel opportunities to control infectious disease outcomes.
Keywords
AP-MS, BioID, effectors, infection biology, interactomics, type-III secretion, Virotrap, SALMONELLA-TYPHIMURIUM, RALSTONIA-SOLANACEARUM, TRIGGERED IMMUNITY, COMPLEXES, IDENTIFICATION, BIOTINYLATION, PLANT, MEMBRANE, TOMATO, PTDINS(4, 5)P-2

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MLA
De Meyer, Margaux, et al. “Keeping in Touch with Type-III Secretion System Effectors : Mass Spectrometry-Based Proteomics to Study Effector-Host Protein-Protein Interactions.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 18, 2020, doi:10.3390/ijms21186891.
APA
De Meyer, M., De Ryck, J., Goormachtig, S., & Van Damme, P. (2020). Keeping in touch with type-III secretion system effectors : mass spectrometry-based proteomics to study effector-host protein-protein interactions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(18). https://doi.org/10.3390/ijms21186891
Chicago author-date
De Meyer, Margaux, Joren De Ryck, Sofie Goormachtig, and Petra Van Damme. 2020. “Keeping in Touch with Type-III Secretion System Effectors : Mass Spectrometry-Based Proteomics to Study Effector-Host Protein-Protein Interactions.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (18). https://doi.org/10.3390/ijms21186891.
Chicago author-date (all authors)
De Meyer, Margaux, Joren De Ryck, Sofie Goormachtig, and Petra Van Damme. 2020. “Keeping in Touch with Type-III Secretion System Effectors : Mass Spectrometry-Based Proteomics to Study Effector-Host Protein-Protein Interactions.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (18). doi:10.3390/ijms21186891.
Vancouver
1.
De Meyer M, De Ryck J, Goormachtig S, Van Damme P. Keeping in touch with type-III secretion system effectors : mass spectrometry-based proteomics to study effector-host protein-protein interactions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020;21(18).
IEEE
[1]
M. De Meyer, J. De Ryck, S. Goormachtig, and P. Van Damme, “Keeping in touch with type-III secretion system effectors : mass spectrometry-based proteomics to study effector-host protein-protein interactions,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 18, 2020.
@article{8676480,
  abstract     = {Manipulation of host cellular processes by translocated bacterial effectors is key to the success of bacterial pathogens and some symbionts. Therefore, a comprehensive understanding of effectors is of critical importance to understand infection biology. It has become increasingly clear that the identification of host protein targets contributes invaluable knowledge to the characterization of effector function during pathogenesis. Recent advances in mapping protein–protein interaction networks by means of mass spectrometry-based interactomics have enabled the identification of host targets at large-scale. In this review, we highlight mass spectrometry-driven proteomics strategies and recent advances to elucidate type-III secretion system effector–host protein–protein interactions. Furthermore, we highlight approaches for defining spatial and temporal effector–host interactions, and discuss possible avenues for studying natively delivered effectors in the context of infection. Overall, the knowledge gained when unravelling effector complexation with host factors will provide novel opportunities to control infectious disease outcomes.},
  articleno    = {6891},
  author       = {De Meyer, Margaux and De Ryck, Joren and Goormachtig, Sofie and Van Damme, Petra},
  issn         = {1422-0067},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
  keywords     = {AP-MS,BioID,effectors,infection biology,interactomics,type-III secretion,Virotrap,SALMONELLA-TYPHIMURIUM,RALSTONIA-SOLANACEARUM,TRIGGERED IMMUNITY,COMPLEXES,IDENTIFICATION,BIOTINYLATION,PLANT,MEMBRANE,TOMATO,PTDINS(4,5)P-2},
  language     = {eng},
  number       = {18},
  pages        = {22},
  title        = {Keeping in touch with type-III secretion system effectors : mass spectrometry-based proteomics to study effector-host protein-protein interactions},
  url          = {http://dx.doi.org/10.3390/ijms21186891},
  volume       = {21},
  year         = {2020},
}

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