
Design and development of a reinforced tubular electrospun construct for the repair of ruptures of deep flexor tendons
- Author
- Nele Pien (UGent) , Ian Peeters (UGent) , Liesbet Deconinck (UGent) , Lana Van Damme (UGent) , Lieven De Wilde (UGent) , Ann Martens (UGent) , Sandra Van Vlierberghe (UGent) , Peter Dubruel (UGent) and Arn Mignon (UGent)
- Organization
- Abstract
- This research aims at developing a more potent solution for deep flexor tendon repair by combining a mechanical and biological approach. A reinforced, multi-layered electrospun tubular construct is developed, composed of three layers: an inner electrospun layer containing an anti-inflammatory component (Naproxen), a middle layer of braided monofilament as reinforcement and an outer electrospun layer containing an anti adhesion component (hyaluronic acid, HA). In a first step, a novel acrylate endcapped urethane-based precursor (AUP) is developed and characterized by measuring molar mass, acrylate content and thermo-stability. The AUP material is benchmarked against commercially available poly(epsilon-caprolactone) (PCL). Next, the materials are processed into multi-layered, tubular constructs with bio-active components (Naproxen and HA) using electrospinning. In vitro assays using human fibroblasts show that incorporation of the bio-active components is successful and not-cytotoxic. Moreover, tensile testing using ex vivo sheep tendons prove that the developed multi-layered constructs fulfill the required strength for tendon repair (i.e. 2.79-3.98 MPa), with an ultimate strength of 8.56 +/- 1.92 MPa and 8.36 +/- 0.57 MPa for PCL and AUP/PCL constructs respectively. In conclusion, by combining a mechanical approach (improved mechanical properties) with the incorporation of bioactive compounds (biological approach), this solution shows its potential for application in deep flexor tendon repair.
- Keywords
- Deep flexor tendon repair, Tubular construct, Electrospinning, Acrylate endcapped urethane-based poly(ε- caprolactone), Ex vivo sheep tendon, MESENCHYMAL STEM-CELLS, MODIFIED KESSLER, SCAFFOLDS, PREVENTION, DELIVERY, SHEATH
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8676118
- MLA
- Pien, Nele, et al. “Design and Development of a Reinforced Tubular Electrospun Construct for the Repair of Ruptures of Deep Flexor Tendons.” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol. 119, 2021, doi:10.1016/j.msec.2020.111504.
- APA
- Pien, N., Peeters, I., Deconinck, L., Van Damme, L., De Wilde, L., Martens, A., … Mignon, A. (2021). Design and development of a reinforced tubular electrospun construct for the repair of ruptures of deep flexor tendons. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 119. https://doi.org/10.1016/j.msec.2020.111504
- Chicago author-date
- Pien, Nele, Ian Peeters, Liesbet Deconinck, Lana Van Damme, Lieven De Wilde, Ann Martens, Sandra Van Vlierberghe, Peter Dubruel, and Arn Mignon. 2021. “Design and Development of a Reinforced Tubular Electrospun Construct for the Repair of Ruptures of Deep Flexor Tendons.” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 119. https://doi.org/10.1016/j.msec.2020.111504.
- Chicago author-date (all authors)
- Pien, Nele, Ian Peeters, Liesbet Deconinck, Lana Van Damme, Lieven De Wilde, Ann Martens, Sandra Van Vlierberghe, Peter Dubruel, and Arn Mignon. 2021. “Design and Development of a Reinforced Tubular Electrospun Construct for the Repair of Ruptures of Deep Flexor Tendons.” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 119. doi:10.1016/j.msec.2020.111504.
- Vancouver
- 1.Pien N, Peeters I, Deconinck L, Van Damme L, De Wilde L, Martens A, et al. Design and development of a reinforced tubular electrospun construct for the repair of ruptures of deep flexor tendons. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS. 2021;119.
- IEEE
- [1]N. Pien et al., “Design and development of a reinforced tubular electrospun construct for the repair of ruptures of deep flexor tendons,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol. 119, 2021.
@article{8676118, abstract = {{This research aims at developing a more potent solution for deep flexor tendon repair by combining a mechanical and biological approach. A reinforced, multi-layered electrospun tubular construct is developed, composed of three layers: an inner electrospun layer containing an anti-inflammatory component (Naproxen), a middle layer of braided monofilament as reinforcement and an outer electrospun layer containing an anti adhesion component (hyaluronic acid, HA). In a first step, a novel acrylate endcapped urethane-based precursor (AUP) is developed and characterized by measuring molar mass, acrylate content and thermo-stability. The AUP material is benchmarked against commercially available poly(epsilon-caprolactone) (PCL). Next, the materials are processed into multi-layered, tubular constructs with bio-active components (Naproxen and HA) using electrospinning. In vitro assays using human fibroblasts show that incorporation of the bio-active components is successful and not-cytotoxic. Moreover, tensile testing using ex vivo sheep tendons prove that the developed multi-layered constructs fulfill the required strength for tendon repair (i.e. 2.79-3.98 MPa), with an ultimate strength of 8.56 +/- 1.92 MPa and 8.36 +/- 0.57 MPa for PCL and AUP/PCL constructs respectively. In conclusion, by combining a mechanical approach (improved mechanical properties) with the incorporation of bioactive compounds (biological approach), this solution shows its potential for application in deep flexor tendon repair.}}, articleno = {{111504}}, author = {{Pien, Nele and Peeters, Ian and Deconinck, Liesbet and Van Damme, Lana and De Wilde, Lieven and Martens, Ann and Van Vlierberghe, Sandra and Dubruel, Peter and Mignon, Arn}}, issn = {{0928-4931}}, journal = {{MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS}}, keywords = {{Deep flexor tendon repair,Tubular construct,Electrospinning,Acrylate endcapped urethane-based poly(ε- caprolactone),Ex vivo sheep tendon,MESENCHYMAL STEM-CELLS,MODIFIED KESSLER,SCAFFOLDS,PREVENTION,DELIVERY,SHEATH}}, language = {{eng}}, pages = {{12}}, title = {{Design and development of a reinforced tubular electrospun construct for the repair of ruptures of deep flexor tendons}}, url = {{http://dx.doi.org/10.1016/j.msec.2020.111504}}, volume = {{119}}, year = {{2021}}, }
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