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Polymerization of the through-pores in HPLC columns for enhanced SEM based assessment of packing order

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Abstract
Recent developments in chip-based column technology, whereby plate heights corresponding to the domain size can be obtained,[1] suggest that it might also be possible to improve the lower minimal plate height limit in packed column HPLC (h≈2dp), down to lower values if feasible improved packing procedures can be developed. In order to allow enhanced understanding of the order in packed HPLC columns, in this work a methodology for immobilizing silica particles is developed based on the polymerization of a monomer and a cross-linker in the interstitial pores of HPLC columns. Subsequent mechanical cutting allows scanning electron microscopy (SEM) based imaging of cross sections of the packed bed over the entire length of the column. In this way the packing efficiency of in-house packed and commercial HPLC columns comprising the same packing material can be compared. The methodology is developed for native silica used in e.g. hydrophilic interaction liquid chromatography and the information obtained is cross-referenced with external porosity measurements obtained via inversed size exclusion approach.

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MLA
Hou, Zhanyao, et al. “Polymerization of the Through-Pores in HPLC Columns for Enhanced SEM Based Assessment of Packing Order.” 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-16), Abstracts, 2020.
APA
Hou, Z., Broeckhoven, K., Desmet, G., & Lynen, F. (2020). Polymerization of the through-pores in HPLC columns for enhanced SEM based assessment of packing order. In 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-16), Abstracts. Ghent, Belgium.
Chicago author-date
Hou, Zhanyao, Ken Broeckhoven, Gert Desmet, and Frederic Lynen. 2020. “Polymerization of the Through-Pores in HPLC Columns for Enhanced SEM Based Assessment of Packing Order.” In 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-16), Abstracts.
Chicago author-date (all authors)
Hou, Zhanyao, Ken Broeckhoven, Gert Desmet, and Frederic Lynen. 2020. “Polymerization of the Through-Pores in HPLC Columns for Enhanced SEM Based Assessment of Packing Order.” In 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-16), Abstracts.
Vancouver
1.
Hou Z, Broeckhoven K, Desmet G, Lynen F. Polymerization of the through-pores in HPLC columns for enhanced SEM based assessment of packing order. In: 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-16), Abstracts. 2020.
IEEE
[1]
Z. Hou, K. Broeckhoven, G. Desmet, and F. Lynen, “Polymerization of the through-pores in HPLC columns for enhanced SEM based assessment of packing order,” in 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-16), Abstracts, Ghent, Belgium, 2020.
@inproceedings{8675705,
  abstract     = {Recent developments in chip-based column technology, whereby plate heights corresponding to the domain size can be obtained,[1] suggest that it might also be possible to improve the lower minimal plate height limit in packed column HPLC (h≈2dp), down to lower values if feasible improved packing procedures can be developed.
In order to allow enhanced understanding of the order in packed HPLC columns, in this work a methodology for immobilizing silica particles is developed based on the polymerization of a monomer and a cross-linker in the interstitial pores of HPLC columns. Subsequent mechanical cutting allows scanning electron microscopy (SEM) based imaging of cross sections of the packed bed over the entire length of the column.
In this way the packing efficiency of in-house packed and commercial HPLC columns comprising the same packing material can be compared. The methodology is developed for native silica used in e.g. hydrophilic interaction liquid chromatography and the information obtained is cross-referenced with external porosity measurements obtained via inversed size exclusion approach.},
  author       = {Hou, Zhanyao and Broeckhoven, Ken and Desmet, Gert and Lynen, Frederic},
  booktitle    = {16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-16), Abstracts},
  language     = {eng},
  location     = {Ghent, Belgium},
  title        = {Polymerization of the through-pores in HPLC columns for enhanced SEM based assessment of packing order},
  year         = {2020},
}