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Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells

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Abstract
The potential utility of RNA interference (RNAi) to control insect pests and viral infections depends largely on the target organism's ability to systemically spread the RNAi response. The efficacy of systemic RNAi varies among insects, though it has been shown to be high in the red flour beetle, Tribolium castaneum. We identified an extracellular RNAi signal that is present in the culture medium of T. castaneum (TcA) cells after treatment with long dsRNA specific for a luciferase reporter gene. Luciferase-specific siRNAs were detected in extracellular vesicles (EVs) that were purified from the culture medium of these dsRNA-treated cells. Furthermore, by measuring the silencing of luciferase expression, we showed that these siRNA-containing EVs can act as an RNAi signal for recipient TcA cells. We have therefore shown that a systemic RNAi response upon dsRNA treatment can be effectively spread through EVs.
Keywords
DOUBLE-STRANDED-RNA, RECEPTOR-MEDIATED ENDOCYTOSIS, ANTIVIRAL DEFENSE, DESERT LOCUST, MESSENGER-RNA, SYSTEMIC RNAI, DSRNA, INITIATION, MICRORNAS, SEQUENCES, dsRNA, Insect, Intercellular communication, RNAi, siRNA, Small RNA

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MLA
Mingels, Lina, et al. “Extracellular Vesicles Spread the RNA Interference Signal of Tribolium Castaneum TcA Cells.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 122, 2020, doi:10.1016/j.ibmb.2020.103377.
APA
Mingels, L., Wynant, N., Santos, D., Peeters, P., Gansemans, Y., Billen, J., … Vanden Broeck, J. (2020). Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 122. https://doi.org/10.1016/j.ibmb.2020.103377
Chicago author-date
Mingels, Lina, Niels Wynant, Dulce Santos, Paulien Peeters, Yannick Gansemans, Johan Billen, Filip Van Nieuwerburgh, and Jozef Vanden Broeck. 2020. “Extracellular Vesicles Spread the RNA Interference Signal of Tribolium Castaneum TcA Cells.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 122. https://doi.org/10.1016/j.ibmb.2020.103377.
Chicago author-date (all authors)
Mingels, Lina, Niels Wynant, Dulce Santos, Paulien Peeters, Yannick Gansemans, Johan Billen, Filip Van Nieuwerburgh, and Jozef Vanden Broeck. 2020. “Extracellular Vesicles Spread the RNA Interference Signal of Tribolium Castaneum TcA Cells.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 122. doi:10.1016/j.ibmb.2020.103377.
Vancouver
1.
Mingels L, Wynant N, Santos D, Peeters P, Gansemans Y, Billen J, et al. Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2020;122.
IEEE
[1]
L. Mingels et al., “Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells,” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 122, 2020.
@article{8675356,
  abstract     = {{The potential utility of RNA interference (RNAi) to control insect pests and viral infections depends largely on the target organism's ability to systemically spread the RNAi response. The efficacy of systemic RNAi varies among insects, though it has been shown to be high in the red flour beetle, Tribolium castaneum. We identified an extracellular RNAi signal that is present in the culture medium of T. castaneum (TcA) cells after treatment with long dsRNA specific for a luciferase reporter gene. Luciferase-specific siRNAs were detected in extracellular vesicles (EVs) that were purified from the culture medium of these dsRNA-treated cells. Furthermore, by measuring the silencing of luciferase expression, we showed that these siRNA-containing EVs can act as an RNAi signal for recipient TcA cells. We have therefore shown that a systemic RNAi response upon dsRNA treatment can be effectively spread through EVs.}},
  articleno    = {{103377}},
  author       = {{Mingels, Lina and Wynant, Niels and Santos, Dulce and Peeters, Paulien and Gansemans, Yannick and Billen, Johan and Van Nieuwerburgh, Filip and Vanden Broeck, Jozef}},
  issn         = {{0965-1748}},
  journal      = {{INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}},
  keywords     = {{DOUBLE-STRANDED-RNA,RECEPTOR-MEDIATED ENDOCYTOSIS,ANTIVIRAL DEFENSE,DESERT LOCUST,MESSENGER-RNA,SYSTEMIC RNAI,DSRNA,INITIATION,MICRORNAS,SEQUENCES,dsRNA,Insect,Intercellular communication,RNAi,siRNA,Small RNA}},
  language     = {{eng}},
  pages        = {{8}},
  title        = {{Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells}},
  url          = {{http://dx.doi.org/10.1016/j.ibmb.2020.103377}},
  volume       = {{122}},
  year         = {{2020}},
}

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