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Characterization of the bacteriophage-derived endolysins PlySs2 and PlySs9 with in vitro lytic activity against bovine mastitis streptococcus uberis

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Abstract
Bovine mastitis, an infection of the cow’s mammary gland, is frequently caused by Streptococcus uberis and causes major economic losses in the dairy industry. The intramammary administration of antibiotics currently remains the predominant preventive and therapeutic measure. These antimicrobial compounds, ofwhich some are consideredcritical in human health care, are frequently applied as dry therapy resulting in their consistent overuse. Therefore, the use of antibiotics in the dairy sector is being questioned. We here identified two endolysins, i.e., PlySs2 and PlySs9, respectively derived from Streptococcus suis serotype-2 and -9 prophages, with lytic activity against S. uberis in an in vitro setting. Both endolysins gave clear lysis zones in spot-on-plate assays and caused a reduction of the optical density in a turbidity reduction assay. In depth characterization identified PlySs9 as the more potent endolysin over PlySs2 with a lower MIC value and about one additional log of killing. PlySs2 and PlySs9 were challenged to a panel of subclinical and clinical S. uberis milk isolates and were both able to lyse all strains tested. Molecular dissection of these endolysins in catalytic and cell wall binding subdomains resulted in major loss of killing and binding activity, respectively. Taken together, we here propose PlySs2 and PlySs9 as candidate compounds to the current antimicrobial arsenal known against bovine mastitis-causing S. uberis as future add-on or replacement strategy to the currently used intramammary antibiotics.
Keywords
PlySs2, PlySs9, bacteriophage-derived endolysins, bovine mastitis, dairy industry, intramammary antibiotics, alternative antimicrobials, STAPHYLOCOCCUS-AUREUS, MASTITIS PATHOGENS, DAIRY-COWS, ANTIBACTERIAL ACTIVITY, STREPTOCOCCUS-UBERIS, CLINICAL MASTITIS, PHAGE LYSIN, MOUSE MODEL, INFECTION

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MLA
Vander Elst, Niels, et al. “Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with in Vitro Lytic Activity against Bovine Mastitis Streptococcus Uberis.” ANTIBIOTICS-BASEL, vol. 9, no. 9, 2020, doi:10.3390/antibiotics9090621.
APA
Vander Elst, N., B. Linden, S., Lavigne, R., Meyer, E., Briers, Y., & C. Nelson, D. (2020). Characterization of the bacteriophage-derived endolysins PlySs2 and PlySs9 with in vitro lytic activity against bovine mastitis streptococcus uberis. ANTIBIOTICS-BASEL, 9(9). https://doi.org/10.3390/antibiotics9090621
Chicago author-date
Vander Elst, Niels, Sara B. Linden, Rob Lavigne, Evelyne Meyer, Yves Briers, and Daniel C. Nelson. 2020. “Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with in Vitro Lytic Activity against Bovine Mastitis Streptococcus Uberis.” ANTIBIOTICS-BASEL 9 (9). https://doi.org/10.3390/antibiotics9090621.
Chicago author-date (all authors)
Vander Elst, Niels, Sara B. Linden, Rob Lavigne, Evelyne Meyer, Yves Briers, and Daniel C. Nelson. 2020. “Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with in Vitro Lytic Activity against Bovine Mastitis Streptococcus Uberis.” ANTIBIOTICS-BASEL 9 (9). doi:10.3390/antibiotics9090621.
Vancouver
1.
Vander Elst N, B. Linden S, Lavigne R, Meyer E, Briers Y, C. Nelson D. Characterization of the bacteriophage-derived endolysins PlySs2 and PlySs9 with in vitro lytic activity against bovine mastitis streptococcus uberis. ANTIBIOTICS-BASEL. 2020;9(9).
IEEE
[1]
N. Vander Elst, S. B. Linden, R. Lavigne, E. Meyer, Y. Briers, and D. C. Nelson, “Characterization of the bacteriophage-derived endolysins PlySs2 and PlySs9 with in vitro lytic activity against bovine mastitis streptococcus uberis,” ANTIBIOTICS-BASEL, vol. 9, no. 9, 2020.
@article{8675161,
  abstract     = {Bovine mastitis, an infection of the cow’s mammary gland, is frequently caused by Streptococcus uberis and causes major economic losses in the dairy industry. The intramammary administration of antibiotics currently remains the predominant preventive and therapeutic measure. These antimicrobial compounds, ofwhich some are consideredcritical in human health care, are frequently applied as dry therapy resulting in their consistent overuse. Therefore, the use of antibiotics in the dairy sector is being questioned. We here identified two endolysins, i.e., PlySs2 and PlySs9, respectively derived from Streptococcus suis serotype-2 and -9 prophages, with lytic activity against S. uberis in an in vitro setting. Both endolysins gave clear lysis zones in spot-on-plate assays and caused a reduction of the optical density in a turbidity reduction assay. In depth characterization identified PlySs9 as the more potent endolysin over PlySs2 with a lower MIC value and about one additional log of killing. PlySs2 and PlySs9 were challenged to a panel of subclinical and clinical S. uberis milk isolates and were both able to lyse all strains tested. Molecular dissection of these endolysins in catalytic and cell wall binding subdomains resulted in major loss of killing and binding activity, respectively. Taken together, we here propose PlySs2 and PlySs9 as candidate compounds to the current antimicrobial arsenal known against bovine mastitis-causing S. uberis as future add-on or replacement strategy to the currently used intramammary antibiotics.},
  articleno    = {621},
  author       = {Vander Elst, Niels and B. Linden, Sara and Lavigne, Rob and Meyer, Evelyne and Briers, Yves and C. Nelson, Daniel},
  issn         = {2079-6382},
  journal      = {ANTIBIOTICS-BASEL},
  keywords     = {PlySs2,PlySs9,bacteriophage-derived endolysins,bovine mastitis,dairy industry,intramammary antibiotics,alternative antimicrobials,STAPHYLOCOCCUS-AUREUS,MASTITIS PATHOGENS,DAIRY-COWS,ANTIBACTERIAL ACTIVITY,STREPTOCOCCUS-UBERIS,CLINICAL MASTITIS,PHAGE LYSIN,MOUSE MODEL,INFECTION},
  language     = {eng},
  number       = {9},
  pages        = {14},
  title        = {Characterization of the bacteriophage-derived endolysins PlySs2 and PlySs9 with in vitro lytic activity against bovine mastitis streptococcus uberis},
  url          = {http://dx.doi.org/10.3390/antibiotics9090621},
  volume       = {9},
  year         = {2020},
}

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