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Burrowing nematodes from Colombia and their relationship with Radopholus similis populations, R. arabocoffeae and R. duriophilus

Gladis Emilia Múnera Uribe, Wim Bert UGent, Andy Vierstraete UGent, Eduardo de la Pena UGent, Maurice Moens UGent and Wilfrida Decraemer UGent (2010) NEMATOLOGY. 12(4). p.619-629
abstract
Two burrowing nematode populations from Colombia were characterised using morphological, morphometric and molecular criteria. The morphological and morphometric characters of the two populations did not differ from those of Radopholus similis. The phylogenetic analysis based on sequence comparison of 18S, D2D3 regions of these populations with those of Radopholus sp., R. similis and other Tylenchomorpha from GenBank confirmed the close molecular relationship between the genus Radopholus and the Hoplolaimidae. The consensus tree based on the comparison of ITS1-5.8-ITS2 rDNA sequences of the Colombian populations with R. similis (many sources), R. arabocoffeae and R. duriophilus from GenBank grouped the Colombian populations in a clade which also included R. similis from different localities. Other R. similis populations grouped in separated clades, which were closely related to the clade containing R. arabocoffeae and R. duriophilus. The high genetic variability among the R. similis populations was observed and confirmed by the high ITS1-5.8-ITS2 sequence divergence within the R. similis populations studied (5.4%) which was comparable to the divergence (4.2-6.9%) between the Radopholus species included in the analysis. These findings and the unresolved phylogenetic placement of R. arabocoffeae and R. duriophilus among R. similis suggests that the burrowing nematode could be a species complex. Limited information on the variability of the 18S and D2D3 region gives an indication of their possible value, besides ITS1-5.8-ITS, as molecular markers within Radopholus. No complete and comprehensible relation was found between the phylogenetic position of the Radopholus populations and their geographical origin or host.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
plantain, 18S, taxonomy, phylogeny, morphometrics, morphology, molecular, rDNA, banana, D2D3, ITS, FRAGMENT-LENGTH-POLYMORPHISM, MULTIPLE SEQUENCE ALIGNMENT, PLANT-PARASITIC NEMATODES, SP-N. NEMATODA, PHYLOGENETIC ANALYSIS, MOLECULAR PHYLOGENY, RIBOSOMAL-RNA, DNA, PRATYLENCHIDAE, TYLENCHIDA
journal title
NEMATOLOGY
Nematology
volume
12
issue
4
pages
619 - 629
Web of Science type
Article
Web of Science id
000284931200009
JCR category
ZOOLOGY
JCR impact factor
0.962 (2010)
JCR rank
74/145 (2010)
JCR quartile
3 (2010)
ISSN
1388-5545
DOI
10.1163/138855410X12628646276005
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
867500
handle
http://hdl.handle.net/1854/LU-867500
date created
2010-02-18 13:39:47
date last changed
2012-01-24 15:27:42
@article{867500,
  abstract     = {Two burrowing nematode populations from Colombia were characterised using morphological, morphometric and molecular criteria. The morphological and morphometric characters of the two populations did not differ from those of Radopholus similis. The phylogenetic analysis based on sequence comparison of 18S, D2D3 regions of these populations with those of Radopholus sp., R. similis and other Tylenchomorpha from GenBank confirmed the close molecular relationship between the genus Radopholus and the Hoplolaimidae. The consensus tree based on the comparison of ITS1-5.8-ITS2 rDNA sequences of the Colombian  populations with R. similis (many sources), R. arabocoffeae and R. duriophilus from GenBank grouped the Colombian populations in a clade which also included R. similis from different localities. Other R. similis populations grouped in separated clades, which were closely related to the clade containing R. arabocoffeae and R. duriophilus. The high genetic variability among the R. similis populations was observed and confirmed by the high ITS1-5.8-ITS2 sequence divergence within the R. similis populations studied (5.4\%) which was comparable to the divergence (4.2-6.9\%) between the Radopholus species included in the analysis. These findings and the unresolved phylogenetic placement of R. arabocoffeae and R. duriophilus among R. similis suggests that the burrowing nematode could be a species complex. Limited information on the variability of the 18S and D2D3 region gives an indication of their possible value, besides ITS1-5.8-ITS, as molecular markers within Radopholus. No complete and comprehensible relation was found between the phylogenetic position of the Radopholus populations and their geographical origin or host.},
  author       = {M{\'u}nera Uribe, Gladis Emilia and Bert, Wim and Vierstraete, Andy and de la Pena, Eduardo and Moens, Maurice and Decraemer, Wilfrida},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keyword      = {plantain,18S,taxonomy,phylogeny,morphometrics,morphology,molecular,rDNA,banana,D2D3,ITS,FRAGMENT-LENGTH-POLYMORPHISM,MULTIPLE SEQUENCE ALIGNMENT,PLANT-PARASITIC NEMATODES,SP-N. NEMATODA,PHYLOGENETIC ANALYSIS,MOLECULAR PHYLOGENY,RIBOSOMAL-RNA,DNA,PRATYLENCHIDAE,TYLENCHIDA},
  language     = {eng},
  number       = {4},
  pages        = {619--629},
  title        = {Burrowing nematodes from Colombia and their relationship with Radopholus similis populations, R. arabocoffeae and R. duriophilus},
  url          = {http://dx.doi.org/10.1163/138855410X12628646276005},
  volume       = {12},
  year         = {2010},
}

Chicago
Múnera Uribe, Gladis Emilia, Wim Bert, Andy Vierstraete, Eduardo de la Pena, Maurice Moens, and Wilfrida Decraemer. 2010. “Burrowing Nematodes from Colombia and Their Relationship with Radopholus Similis Populations, R. Arabocoffeae and R. Duriophilus.” Nematology 12 (4): 619–629.
APA
Múnera Uribe, G. E., Bert, W., Vierstraete, A., de la Pena, E., Moens, M., & Decraemer, W. (2010). Burrowing nematodes from Colombia and their relationship with Radopholus similis populations, R. arabocoffeae and R. duriophilus. NEMATOLOGY, 12(4), 619–629.
Vancouver
1.
Múnera Uribe GE, Bert W, Vierstraete A, de la Pena E, Moens M, Decraemer W. Burrowing nematodes from Colombia and their relationship with Radopholus similis populations, R. arabocoffeae and R. duriophilus. NEMATOLOGY. 2010;12(4):619–29.
MLA
Múnera Uribe, Gladis Emilia, Wim Bert, Andy Vierstraete, et al. “Burrowing Nematodes from Colombia and Their Relationship with Radopholus Similis Populations, R. Arabocoffeae and R. Duriophilus.” NEMATOLOGY 12.4 (2010): 619–629. Print.