Heat tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora
(2010) BIOCONTROL. 55(3). p.423-434- abstract
- Quality of biological control products based on entomopathogenic nematodes can be severely damaged due to exposure to high temperature surpassing 40A degrees C. The study screened 36 natural populations and 18 hybrid or inbred strains of Heterorhabditis bacteriophora for their response to high temperature. Nematodes were tested with or without prior adaptation to heat at 35A degrees C for 3 h. Five strains of H. indica and one of H. megidis were also included. Molecular identification using nuclear ribosomal DNA sequences confirmed the designation to the three Heterorhabditis spp. The mean tolerated temperature ranged from 33.3A degrees C to 40.1A degrees C for non-adapted and from 34.8A degrees C to 39.2A degrees C for adapted strain populations. H. indica was the most tolerant, followed by H. bacteriophora and H. megidis. No correlation was recorded between tolerance assessed with and without adaptation to heat, implying that different genes are involved. Correlation between heat tolerance and mean annual temperature at place of origin of the strains was weak. A high variability in tolerance among strains and the relatively high heritability (hA(2)A = 0.68) for the adapted heat tolerance recorded for H. bacteriophora provide an excellent foundation for future selective breeding with the objective to enhance heat tolerance of H. bacteriophora.
Please use this url to cite or link to this publication:
http://hdl.handle.net/1854/LU-867423
- author
- John Mukuka, Olaf Strauch, Lieven Waeyenberge, Nicole Viaene, Maurice Moens UGent and Ralf-Udo Ehlers
- organization
- year
- 2010
- type
- journalArticle (original)
- publication status
- published
- subject
- keyword
- H. megidis, Biological control, selective breeding, adaptation, H. indica, enhanced heat tolerance, LOW-TEMPERATURE ACTIVITY, STEINERNEMA-CARPOCAPSAE, NATURAL-POPULATIONS, STRESS TOLERANCE, MASS-PRODUCTION, REPRODUCTION, SHOCK, INFECTIVITY, PERSISTENCE, RHABDITIDA
- journal title
- BIOCONTROL
- BioControl
- volume
- 55
- issue
- 3
- pages
- 423 - 434
- Web of Science type
- Article
- Web of Science id
- 000277955400011
- JCR category
- ENTOMOLOGY
- JCR impact factor
- 2.191 (2010)
- JCR rank
- 10/83 (2010)
- JCR quartile
- 1 (2010)
- ISSN
- 1386-6141
- DOI
- 10.1007/s10526-009-9255-4
- language
- English
- UGent publication?
- no
- classification
- A1
- copyright statement
- I have transferred the copyright for this publication to the publisher
- id
- 867423
- handle
- http://hdl.handle.net/1854/LU-867423
- date created
- 2010-02-18 13:19:05
- date last changed
- 2016-12-19 15:46:37
@article{867423, abstract = {Quality of biological control products based on entomopathogenic nematodes can be severely damaged due to exposure to high temperature surpassing 40A degrees C. The study screened 36 natural populations and 18 hybrid or inbred strains of Heterorhabditis bacteriophora for their response to high temperature. Nematodes were tested with or without prior adaptation to heat at 35A degrees C for 3 h. Five strains of H. indica and one of H. megidis were also included. Molecular identification using nuclear ribosomal DNA sequences confirmed the designation to the three Heterorhabditis spp. The mean tolerated temperature ranged from 33.3A degrees C to 40.1A degrees C for non-adapted and from 34.8A degrees C to 39.2A degrees C for adapted strain populations. H. indica was the most tolerant, followed by H. bacteriophora and H. megidis. No correlation was recorded between tolerance assessed with and without adaptation to heat, implying that different genes are involved. Correlation between heat tolerance and mean annual temperature at place of origin of the strains was weak. A high variability in tolerance among strains and the relatively high heritability (hA(2)A = 0.68) for the adapted heat tolerance recorded for H. bacteriophora provide an excellent foundation for future selective breeding with the objective to enhance heat tolerance of H. bacteriophora.}, author = {Mukuka, John and Strauch, Olaf and Waeyenberge, Lieven and Viaene, Nicole and Moens, Maurice and Ehlers, Ralf-Udo}, issn = {1386-6141}, journal = {BIOCONTROL}, keyword = {H. megidis,Biological control,selective breeding,adaptation,H. indica,enhanced heat tolerance,LOW-TEMPERATURE ACTIVITY,STEINERNEMA-CARPOCAPSAE,NATURAL-POPULATIONS,STRESS TOLERANCE,MASS-PRODUCTION,REPRODUCTION,SHOCK,INFECTIVITY,PERSISTENCE,RHABDITIDA}, language = {eng}, number = {3}, pages = {423--434}, title = {Heat tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora}, url = {http://dx.doi.org/10.1007/s10526-009-9255-4}, volume = {55}, year = {2010}, }
- Chicago
- Mukuka, John, Olaf Strauch, Lieven Waeyenberge, Nicole Viaene, Maurice Moens, and Ralf-Udo Ehlers. 2010. “Heat Tolerance Among Different Strains of the Entomopathogenic Nematode Heterorhabditis Bacteriophora.” Biocontrol 55 (3): 423–434.
- APA
- Mukuka, J., Strauch, O., Waeyenberge, L., Viaene, N., Moens, M., & Ehlers, R.-U. (2010). Heat tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora. BIOCONTROL, 55(3), 423–434.
- Vancouver
- 1.Mukuka J, Strauch O, Waeyenberge L, Viaene N, Moens M, Ehlers R-U. Heat tolerance among different strains of the entomopathogenic nematode Heterorhabditis bacteriophora. BIOCONTROL. 2010;55(3):423–34.
- MLA
- Mukuka, John, Olaf Strauch, Lieven Waeyenberge, et al. “Heat Tolerance Among Different Strains of the Entomopathogenic Nematode Heterorhabditis Bacteriophora.” BIOCONTROL 55.3 (2010): 423–434. Print.