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Biosynthesis and antimicrobial activity of pseudodesmin and viscosinamide cyclic lipopeptides produced by pseudomonads associated with the cocoyam rhizosphere

Feyisara Eyiwumi Oni (UGent) , Niels Geudens (UGent) , Amayana Adiobo, Olumide Owolabi Omoboye, Elsie Ayamoh Enow, Joseph T. Onyeka, Ayodeji E. Salami, Rene De Mot, José Martins (UGent) and Monica Höfte (UGent)
(2020) MICROORGANISMS. 8(7).
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Abstract
Pseudomonascyclic lipopeptides (CLPs) are encoded non-ribosomally by biosynthetic gene clusters (BGCs) and possess diverse biological activities. In this study, we conducted chemical structure and BGC analyses with antimicrobial activity assays for two CLPs produced byPseudomonasstrains isolated from the cocoyam rhizosphere in Cameroon and Nigeria. LC-MS and NMR analyses showed that thePseudomonassp. COR52 and A2W4.9 produce pseudodesmin and viscosinamide, respectively. These CLPs belong to the Viscosin group characterized by a nonapeptidic moiety with a 7-membered macrocycle. Similar to other Viscosin-group CLPs, the initiatory non-ribosomal peptide synthetase (NRPS) gene of the viscosinamide BGC is situated remotely from the other two NRPS genes. In contrast, the pseudodesmin genes are all clustered in a single genomic locus. Nano- to micromolar levels of pseudodesmin and viscosinamide led to the hyphal distortion and/or disintegration ofRhizoctonia solaniAG2-2 andPythium myriotylumCMR1, whereas similar levels of White Line-Inducing Principle (WLIP), another member of the Viscosin group, resulted in complete lysis of both soil-borne phytopathogens. In addition to the identification of the biosynthetic genes of these two CLPs and the demonstration of their interaction with soil-borne pathogens, this study provides further insights regarding evolutionary divergence within the Viscosin group.
Keywords
viscosin group, WLIP, evolution, CLP perception, defense response, Pythium myriotylum, Rhizoctonia solani, swarming motility, LINE-INDUCING PRINCIPLE, FLUORESCENS, LIPODEPSIPEPTIDES, STRAIN, ROOT, BIOSURFACTANT, ANTIBIOTICS, ANTIFUNGAL, DIVERSITY, INTERPLAY

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MLA
Oni, Feyisara Eyiwumi, et al. “Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere.” MICROORGANISMS, vol. 8, no. 7, 2020, doi:10.3390/microorganisms8071079.
APA
Oni, F. E., Geudens, N., Adiobo, A., Omoboye, O. O., Ayamoh Enow, E., Onyeka, J. T., … Höfte, M. (2020). Biosynthesis and antimicrobial activity of pseudodesmin and viscosinamide cyclic lipopeptides produced by pseudomonads associated with the cocoyam rhizosphere. MICROORGANISMS, 8(7). https://doi.org/10.3390/microorganisms8071079
Chicago author-date
Oni, Feyisara Eyiwumi, Niels Geudens, Amayana Adiobo, Olumide Owolabi Omoboye, Elsie Ayamoh Enow, Joseph T. Onyeka, Ayodeji E. Salami, Rene De Mot, José Martins, and Monica Höfte. 2020. “Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere.” MICROORGANISMS 8 (7). https://doi.org/10.3390/microorganisms8071079.
Chicago author-date (all authors)
Oni, Feyisara Eyiwumi, Niels Geudens, Amayana Adiobo, Olumide Owolabi Omoboye, Elsie Ayamoh Enow, Joseph T. Onyeka, Ayodeji E. Salami, Rene De Mot, José Martins, and Monica Höfte. 2020. “Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere.” MICROORGANISMS 8 (7). doi:10.3390/microorganisms8071079.
Vancouver
1.
Oni FE, Geudens N, Adiobo A, Omoboye OO, Ayamoh Enow E, Onyeka JT, et al. Biosynthesis and antimicrobial activity of pseudodesmin and viscosinamide cyclic lipopeptides produced by pseudomonads associated with the cocoyam rhizosphere. MICROORGANISMS. 2020;8(7).
IEEE
[1]
F. E. Oni et al., “Biosynthesis and antimicrobial activity of pseudodesmin and viscosinamide cyclic lipopeptides produced by pseudomonads associated with the cocoyam rhizosphere,” MICROORGANISMS, vol. 8, no. 7, 2020.
@article{8673485,
  abstract     = {{Pseudomonascyclic lipopeptides (CLPs) are encoded non-ribosomally by biosynthetic gene clusters (BGCs) and possess diverse biological activities. In this study, we conducted chemical structure and BGC analyses with antimicrobial activity assays for two CLPs produced byPseudomonasstrains isolated from the cocoyam rhizosphere in Cameroon and Nigeria. LC-MS and NMR analyses showed that thePseudomonassp. COR52 and A2W4.9 produce pseudodesmin and viscosinamide, respectively. These CLPs belong to the Viscosin group characterized by a nonapeptidic moiety with a 7-membered macrocycle. Similar to other Viscosin-group CLPs, the initiatory non-ribosomal peptide synthetase (NRPS) gene of the viscosinamide BGC is situated remotely from the other two NRPS genes. In contrast, the pseudodesmin genes are all clustered in a single genomic locus. Nano- to micromolar levels of pseudodesmin and viscosinamide led to the hyphal distortion and/or disintegration ofRhizoctonia solaniAG2-2 andPythium myriotylumCMR1, whereas similar levels of White Line-Inducing Principle (WLIP), another member of the Viscosin group, resulted in complete lysis of both soil-borne phytopathogens. In addition to the identification of the biosynthetic genes of these two CLPs and the demonstration of their interaction with soil-borne pathogens, this study provides further insights regarding evolutionary divergence within the Viscosin group.}},
  articleno    = {{1079}},
  author       = {{Oni, Feyisara Eyiwumi and Geudens, Niels and Adiobo, Amayana and Omoboye, Olumide Owolabi and Ayamoh Enow, Elsie and Onyeka, Joseph T. and Salami, Ayodeji E. and De Mot, Rene and Martins, José and Höfte, Monica}},
  issn         = {{2076-2607}},
  journal      = {{MICROORGANISMS}},
  keywords     = {{viscosin group,WLIP,evolution,CLP perception,defense response,Pythium myriotylum,Rhizoctonia solani,swarming motility,LINE-INDUCING PRINCIPLE,FLUORESCENS,LIPODEPSIPEPTIDES,STRAIN,ROOT,BIOSURFACTANT,ANTIBIOTICS,ANTIFUNGAL,DIVERSITY,INTERPLAY}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{29}},
  title        = {{Biosynthesis and antimicrobial activity of pseudodesmin and viscosinamide cyclic lipopeptides produced by pseudomonads associated with the cocoyam rhizosphere}},
  url          = {{http://doi.org/10.3390/microorganisms8071079}},
  volume       = {{8}},
  year         = {{2020}},
}
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