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Efficacy of heterologous prime-boost vaccination with H3N2 influenza viruses in pre-immune individuals : studies in the pig model

Sharon Chepkwony (UGent) , Anna Parys (UGent) , Elien Vandoorn (UGent) , Koen Chiers (UGent) and Kristien Van Reeth (UGent)
(2020) VIRUSES-BASEL. 12(9).
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Abstract
In a previous study in influenza-naïve pigs, heterologous prime-boost vaccination with monovalent, adjuvanted whole inactivated vaccines (WIV) based on the European swine influenza A virus (SwIAV) strain, A/swine/Gent/172/2008 (G08), followed by the US SwIAV strain, A/swine/Pennsylvania/A01076777/2010 (PA10), was shown to induce broadly cross-reactive hemagglutination inhibition (HI) antibodies against 12 out of 15 antigenically distinct H3N2 influenza strains. Here, we used the pig model to examine the efficacy of that particular heterologous prime-boost vaccination regimen, in individuals with pre-existing infection-immunity. Pigs were first inoculated intranasally with the human H3N2 strain, A/Nanchang/933/1995. Seven weeks later, they were vaccinated intramuscularly with G08 followed by PA10 or vice versa. We examined serum antibody responses against the hemagglutinin and neuraminidase, and antibody-secreting cell (ASC) responses in peripheral blood, draining lymph nodes, and nasal mucosa (NMC), in ELISPOT assays. Vaccination induced up to 10-fold higher HI antibody titers than in naïve pigs, with broader cross-reactivity, and protection against challenge with an antigenically distant H3N2 strain. It also boosted ASC responses in lymph nodes and NMC. Our results show that intramuscular administration of WIV can lead to enhanced antibody responses and cross-reactivity in pre-immune subjects, and recall of ASC responses in lymph nodes and NMC.
Keywords
Virology, Infectious Diseases, influenza, swine, humans, pre-existing immunity, vaccination, heterologous prime-boost, antibody cross-reactivity, A(H3N2) VARIANT VIRUS, IMMUNE-RESPONSES, ANTIGENICALLY DISTINCT, AGRICULTURAL FAIRS, GENETIC EVOLUTION, A VIRUSES, B-CELLS, SWINE, INFECTION, PROTECTION

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MLA
Chepkwony, Sharon, et al. “Efficacy of Heterologous Prime-Boost Vaccination with H3N2 Influenza Viruses in Pre-Immune Individuals : Studies in the Pig Model.” VIRUSES-BASEL, vol. 12, no. 9, 2020, doi:10.3390/v12090968.
APA
Chepkwony, S., Parys, A., Vandoorn, E., Chiers, K., & Van Reeth, K. (2020). Efficacy of heterologous prime-boost vaccination with H3N2 influenza viruses in pre-immune individuals : studies in the pig model. VIRUSES-BASEL, 12(9). https://doi.org/10.3390/v12090968
Chicago author-date
Chepkwony, Sharon, Anna Parys, Elien Vandoorn, Koen Chiers, and Kristien Van Reeth. 2020. “Efficacy of Heterologous Prime-Boost Vaccination with H3N2 Influenza Viruses in Pre-Immune Individuals : Studies in the Pig Model.” VIRUSES-BASEL 12 (9). https://doi.org/10.3390/v12090968.
Chicago author-date (all authors)
Chepkwony, Sharon, Anna Parys, Elien Vandoorn, Koen Chiers, and Kristien Van Reeth. 2020. “Efficacy of Heterologous Prime-Boost Vaccination with H3N2 Influenza Viruses in Pre-Immune Individuals : Studies in the Pig Model.” VIRUSES-BASEL 12 (9). doi:10.3390/v12090968.
Vancouver
1.
Chepkwony S, Parys A, Vandoorn E, Chiers K, Van Reeth K. Efficacy of heterologous prime-boost vaccination with H3N2 influenza viruses in pre-immune individuals : studies in the pig model. VIRUSES-BASEL. 2020;12(9).
IEEE
[1]
S. Chepkwony, A. Parys, E. Vandoorn, K. Chiers, and K. Van Reeth, “Efficacy of heterologous prime-boost vaccination with H3N2 influenza viruses in pre-immune individuals : studies in the pig model,” VIRUSES-BASEL, vol. 12, no. 9, 2020.
@article{8673377,
  abstract     = {{In a previous study in influenza-naïve pigs, heterologous prime-boost vaccination with monovalent, adjuvanted whole inactivated vaccines (WIV) based on the European swine influenza A virus (SwIAV) strain, A/swine/Gent/172/2008 (G08), followed by the US SwIAV strain, A/swine/Pennsylvania/A01076777/2010 (PA10), was shown to induce broadly cross-reactive hemagglutination inhibition (HI) antibodies against 12 out of 15 antigenically distinct H3N2 influenza strains. Here, we used the pig model to examine the efficacy of that particular heterologous prime-boost vaccination regimen, in individuals with pre-existing infection-immunity. Pigs were first inoculated intranasally with the human H3N2 strain, A/Nanchang/933/1995. Seven weeks later, they were vaccinated intramuscularly with G08 followed by PA10 or vice versa. We examined serum antibody responses against the hemagglutinin and neuraminidase, and antibody-secreting cell (ASC) responses in peripheral blood, draining lymph nodes, and nasal mucosa (NMC), in ELISPOT assays. Vaccination induced up to 10-fold higher HI antibody titers than in naïve pigs, with broader cross-reactivity, and protection against challenge with an antigenically distant H3N2 strain. It also boosted ASC responses in lymph nodes and NMC. Our results show that intramuscular administration of WIV can lead to enhanced antibody responses and cross-reactivity in pre-immune subjects, and recall of ASC responses in lymph nodes and NMC.}},
  articleno    = {{968}},
  author       = {{Chepkwony, Sharon and Parys, Anna and Vandoorn, Elien and Chiers, Koen and Van Reeth, Kristien}},
  issn         = {{1999-4915}},
  journal      = {{VIRUSES-BASEL}},
  keywords     = {{Virology,Infectious Diseases,influenza,swine,humans,pre-existing immunity,vaccination,heterologous prime-boost,antibody cross-reactivity,A(H3N2) VARIANT VIRUS,IMMUNE-RESPONSES,ANTIGENICALLY DISTINCT,AGRICULTURAL FAIRS,GENETIC EVOLUTION,A VIRUSES,B-CELLS,SWINE,INFECTION,PROTECTION}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{20}},
  title        = {{Efficacy of heterologous prime-boost vaccination with H3N2 influenza viruses in pre-immune individuals : studies in the pig model}},
  url          = {{http://dx.doi.org/10.3390/v12090968}},
  volume       = {{12}},
  year         = {{2020}},
}

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