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Silanized luminescent quantum dots for the simultaneous multicolor lateral flow immunoassay of two mycotoxins

Olga Goryacheva, Chris Guhrenz, Kristian Schneider, Natalia Beloglazova (UGent) , Irina Yu Goryacheva, Sarah De Saeger (UGent) and Nikolai Gaponik
(2020) ACS APPLIED MATERIALS & INTERFACES. 12(22). p.24575-24584
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Abstract
A critical point for the successful development of a fluorescent quantum dot (QD)-based immunoassay is maintaining the high fluorescence quantum yield of QDs during hydrophilization and bioconjugation. In this paper, we carefully designed CdSe/CdS and CdSe/CdS/ZnS core-shell heterostructures and extended them with silica coating of different surface composition allowing preservation of fluorescence quantum yield as high as 70% in aqueous media. The silanized QDs containing epoxy and carboxy surface groups were bioconjugated with monoclonal antibodies. The synthesized fluorescent conjugates were used in a multicolor lateral flow immunoassay for simultaneous determination of two mycotoxins. Zearalenone and deoxynivalenol were chosen as a proof of concept. Cutoff levels for the zearalenone and deoxynivalenol detection were adjusted to be at 40 and 400 mu g kg(-)(1), respectively, complying with the European Commission regulation. Validation of the developed test was performed by analysis of 34 naturally contaminated maize and wheat samples; as a confirmatory method, LC-MS/MS was used.
Keywords
ON PHOTOELECTROCHEMICAL IMMUNOASSAY, HIGHLY LUMINESCENT, NANOPARTICLES, NANOCRYSTALS, EFFICIENT, EMISSION, GROWTH, Quantum dots, silica coating, simultaneous lateral flow immunoassay, detection, mycotoxins, food safety

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MLA
Goryacheva, Olga, et al. “Silanized Luminescent Quantum Dots for the Simultaneous Multicolor Lateral Flow Immunoassay of Two Mycotoxins.” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 22, 2020, pp. 24575–84, doi:10.1021/acsami.0c05099.
APA
Goryacheva, O., Guhrenz, C., Schneider, K., Beloglazova, N., Goryacheva, I. Y., De Saeger, S., & Gaponik, N. (2020). Silanized luminescent quantum dots for the simultaneous multicolor lateral flow immunoassay of two mycotoxins. ACS APPLIED MATERIALS & INTERFACES, 12(22), 24575–24584. https://doi.org/10.1021/acsami.0c05099
Chicago author-date
Goryacheva, Olga, Chris Guhrenz, Kristian Schneider, Natalia Beloglazova, Irina Yu Goryacheva, Sarah De Saeger, and Nikolai Gaponik. 2020. “Silanized Luminescent Quantum Dots for the Simultaneous Multicolor Lateral Flow Immunoassay of Two Mycotoxins.” ACS APPLIED MATERIALS & INTERFACES 12 (22): 24575–84. https://doi.org/10.1021/acsami.0c05099.
Chicago author-date (all authors)
Goryacheva, Olga, Chris Guhrenz, Kristian Schneider, Natalia Beloglazova, Irina Yu Goryacheva, Sarah De Saeger, and Nikolai Gaponik. 2020. “Silanized Luminescent Quantum Dots for the Simultaneous Multicolor Lateral Flow Immunoassay of Two Mycotoxins.” ACS APPLIED MATERIALS & INTERFACES 12 (22): 24575–24584. doi:10.1021/acsami.0c05099.
Vancouver
1.
Goryacheva O, Guhrenz C, Schneider K, Beloglazova N, Goryacheva IY, De Saeger S, et al. Silanized luminescent quantum dots for the simultaneous multicolor lateral flow immunoassay of two mycotoxins. ACS APPLIED MATERIALS & INTERFACES. 2020;12(22):24575–84.
IEEE
[1]
O. Goryacheva et al., “Silanized luminescent quantum dots for the simultaneous multicolor lateral flow immunoassay of two mycotoxins,” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 22, pp. 24575–24584, 2020.
@article{8673130,
  abstract     = {{A critical point for the successful development of a fluorescent quantum dot (QD)-based immunoassay is maintaining the high fluorescence quantum yield of QDs during hydrophilization and bioconjugation. In this paper, we carefully designed CdSe/CdS and CdSe/CdS/ZnS core-shell heterostructures and extended them with silica coating of different surface composition allowing preservation of fluorescence quantum yield as high as 70% in aqueous media. The silanized QDs containing epoxy and carboxy surface groups were bioconjugated with monoclonal antibodies. The synthesized fluorescent conjugates were used in a multicolor lateral flow immunoassay for simultaneous determination of two mycotoxins. Zearalenone and deoxynivalenol were chosen as a proof of concept. Cutoff levels for the zearalenone and deoxynivalenol detection were adjusted to be at 40 and 400 mu g kg(-)(1), respectively, complying with the European Commission regulation. Validation of the developed test was performed by analysis of 34 naturally contaminated maize and wheat samples; as a confirmatory method, LC-MS/MS was used.}},
  author       = {{Goryacheva, Olga and Guhrenz, Chris and Schneider, Kristian and Beloglazova, Natalia and Goryacheva, Irina Yu and De Saeger, Sarah and Gaponik, Nikolai}},
  issn         = {{1944-8244}},
  journal      = {{ACS APPLIED MATERIALS & INTERFACES}},
  keywords     = {{ON PHOTOELECTROCHEMICAL IMMUNOASSAY,HIGHLY LUMINESCENT,NANOPARTICLES,NANOCRYSTALS,EFFICIENT,EMISSION,GROWTH,Quantum dots,silica coating,simultaneous lateral flow immunoassay,detection,mycotoxins,food safety}},
  language     = {{eng}},
  number       = {{22}},
  pages        = {{24575--24584}},
  title        = {{Silanized luminescent quantum dots for the simultaneous multicolor lateral flow immunoassay of two mycotoxins}},
  url          = {{http://doi.org/10.1021/acsami.0c05099}},
  volume       = {{12}},
  year         = {{2020}},
}
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