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Biofilm mode of cultivation leads to an improvement of the entomotoxic patterns of two Aspergillus species

Frederic Francis, Florent Druart, José Diana Di Mavungu (UGent) , Marthe De Boevre (UGent) , Sarah De Saeger (UGent) and Frank Delvigne
(2020) MICROORGANISMS. 8(5).
Author
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Abstract
Two fungi, i.e., Aspergillus flavus Link and Aspergillus oryzae (Ahlb.) E. Cohn, were cultivated according to two methodologies, namely submerged and biofilm cultures with the primary aim to use their secondary metabolites the supernatant CL50, and CL90 varied between 1.3% (v/v) to 12.7% (v/v) for incubation times from 24 to 72 h. While the A. flavus supernatant entomotoxicity was higher than this of A. oryzae, the biofilm culture application increased the efficiency of the former. Proteomic analysis of the supernatants revealed discrepancies among the two species and modes of cultivation. Furthermore, the secondary metabolite profiles of both Aspergillus cultures were verified. Aspergillic acid, beta-cyclopiazonic acid, cyclopiazonic acid, ferrineospergillin, flavacol, and spermadin A were most predominant. Generally, these secondary metabolites were present in higher concentrations in the supernatants of A. flavus and biofilm cultures. These molecular identifications correlated positively with entomotoxic activity. Noteworthy, the absence of carcinogenic aflatoxins was remarkable, and it will allow further valorization to produce A. flavus to develop potential biopesticides.
Keywords
Aspergillus flavus, Aspergillus oryzae, secondary metabolites, Culex quinquefasciatus, submerged culture, biofilm, mycotoxins, biological control, entomopathogenic microorganism, SOLID-STATE CULTURE, COA-TRANSFERASE, GENE-CLUSTER, ACID, BIOSYNTHESIS, AFLATOXINS, METABOLITES, PROTEINS

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MLA
Francis, Frederic, et al. “Biofilm Mode of Cultivation Leads to an Improvement of the Entomotoxic Patterns of Two Aspergillus Species.” MICROORGANISMS, vol. 8, no. 5, 2020, doi:10.3390/microorganisms8050705.
APA
Francis, F., Druart, F., Diana Di Mavungu, J., De Boevre, M., De Saeger, S., & Delvigne, F. (2020). Biofilm mode of cultivation leads to an improvement of the entomotoxic patterns of two Aspergillus species. MICROORGANISMS, 8(5). https://doi.org/10.3390/microorganisms8050705
Chicago author-date
Francis, Frederic, Florent Druart, José Diana Di Mavungu, Marthe De Boevre, Sarah De Saeger, and Frank Delvigne. 2020. “Biofilm Mode of Cultivation Leads to an Improvement of the Entomotoxic Patterns of Two Aspergillus Species.” MICROORGANISMS 8 (5). https://doi.org/10.3390/microorganisms8050705.
Chicago author-date (all authors)
Francis, Frederic, Florent Druart, José Diana Di Mavungu, Marthe De Boevre, Sarah De Saeger, and Frank Delvigne. 2020. “Biofilm Mode of Cultivation Leads to an Improvement of the Entomotoxic Patterns of Two Aspergillus Species.” MICROORGANISMS 8 (5). doi:10.3390/microorganisms8050705.
Vancouver
1.
Francis F, Druart F, Diana Di Mavungu J, De Boevre M, De Saeger S, Delvigne F. Biofilm mode of cultivation leads to an improvement of the entomotoxic patterns of two Aspergillus species. MICROORGANISMS. 2020;8(5).
IEEE
[1]
F. Francis, F. Druart, J. Diana Di Mavungu, M. De Boevre, S. De Saeger, and F. Delvigne, “Biofilm mode of cultivation leads to an improvement of the entomotoxic patterns of two Aspergillus species,” MICROORGANISMS, vol. 8, no. 5, 2020.
@article{8673119,
  abstract     = {{Two fungi, i.e., Aspergillus flavus Link and Aspergillus oryzae (Ahlb.) E. Cohn, were cultivated according to two methodologies, namely submerged and biofilm cultures with the primary aim to use their secondary metabolites the supernatant CL50, and CL90 varied between 1.3% (v/v) to 12.7% (v/v) for incubation times from 24 to 72 h. While the A. flavus supernatant entomotoxicity was higher than this of A. oryzae, the biofilm culture application increased the efficiency of the former. Proteomic analysis of the supernatants revealed discrepancies among the two species and modes of cultivation. Furthermore, the secondary metabolite profiles of both Aspergillus cultures were verified. Aspergillic acid, beta-cyclopiazonic acid, cyclopiazonic acid, ferrineospergillin, flavacol, and spermadin A were most predominant. Generally, these secondary metabolites were present in higher concentrations in the supernatants of A. flavus and biofilm cultures. These molecular identifications correlated positively with entomotoxic activity. Noteworthy, the absence of carcinogenic aflatoxins was remarkable, and it will allow further valorization to produce A. flavus to develop potential biopesticides.}},
  articleno    = {{705}},
  author       = {{Francis, Frederic and Druart, Florent and Diana Di Mavungu, José and De Boevre, Marthe and De Saeger, Sarah and Delvigne, Frank}},
  issn         = {{2076-2607}},
  journal      = {{MICROORGANISMS}},
  keywords     = {{Aspergillus flavus,Aspergillus oryzae,secondary metabolites,Culex quinquefasciatus,submerged culture,biofilm,mycotoxins,biological control,entomopathogenic microorganism,SOLID-STATE CULTURE,COA-TRANSFERASE,GENE-CLUSTER,ACID,BIOSYNTHESIS,AFLATOXINS,METABOLITES,PROTEINS}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{14}},
  title        = {{Biofilm mode of cultivation leads to an improvement of the entomotoxic patterns of two Aspergillus species}},
  url          = {{http://dx.doi.org/10.3390/microorganisms8050705}},
  volume       = {{8}},
  year         = {{2020}},
}
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