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Parallel validation of a green-solvent extraction method and quantitative estimation of multi-mycotoxins in staple cereals using LC-MS/MS

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Abstract
In this study, 15 different mycotoxins were estimated in three staple cereals from selected agro-ecological regions in Nigeria using a 'novel' green extraction method, pressurized hot water extraction (PHWE) in comparison to a conventional solvent extraction method. Discrimination of the results of PHWE and solvent extraction using principal component analysis (PCA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA) did not yield any differential clustering patterns. All maize samples (n=16), 32% (n=38) of sorghum and 35% (n=37) of millet samples were positive for at least one of the 15 tested mycotoxins. Contamination levels for the cereals were higher in the warm humid rain forest region and gradually decreased towards the hot and arid region in the north of the country. The results demonstrate the applicability of PHWE as a possible alternative extraction method to conventional methods of extraction, which are solvent based.
Keywords
5 AGROECOLOGICAL ZONES, TANDEM MASS-SPECTROMETRY, FUMONISIN B1, OCHRATOXIN-A, AFLATOXIN CONTAMINATION, ALTERNARIA MYCOTOXINS, MAIZE, EXPOSURE, RISK, ZEARALENONE

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MLA
Gbashi, Sefater, et al. “Parallel Validation of a Green-Solvent Extraction Method and Quantitative Estimation of Multi-Mycotoxins in Staple Cereals Using LC-MS/MS.” SCIENTIFIC REPORTS, vol. 10, 2020, doi:10.1038/s41598-020-66787-z.
APA
Gbashi, S., Njobeh, P. B., Madala, N. E., De Boevre, M., Kagot, V. O., & De Saeger, S. (2020). Parallel validation of a green-solvent extraction method and quantitative estimation of multi-mycotoxins in staple cereals using LC-MS/MS. SCIENTIFIC REPORTS, 10. https://doi.org/10.1038/s41598-020-66787-z
Chicago author-date
Gbashi, Sefater, Patrick Berka Njobeh, Ntakadzeni Edwin Madala, Marthe De Boevre, Victor Omondi Kagot, and Sarah De Saeger. 2020. “Parallel Validation of a Green-Solvent Extraction Method and Quantitative Estimation of Multi-Mycotoxins in Staple Cereals Using LC-MS/MS.” SCIENTIFIC REPORTS 10. https://doi.org/10.1038/s41598-020-66787-z.
Chicago author-date (all authors)
Gbashi, Sefater, Patrick Berka Njobeh, Ntakadzeni Edwin Madala, Marthe De Boevre, Victor Omondi Kagot, and Sarah De Saeger. 2020. “Parallel Validation of a Green-Solvent Extraction Method and Quantitative Estimation of Multi-Mycotoxins in Staple Cereals Using LC-MS/MS.” SCIENTIFIC REPORTS 10. doi:10.1038/s41598-020-66787-z.
Vancouver
1.
Gbashi S, Njobeh PB, Madala NE, De Boevre M, Kagot VO, De Saeger S. Parallel validation of a green-solvent extraction method and quantitative estimation of multi-mycotoxins in staple cereals using LC-MS/MS. SCIENTIFIC REPORTS. 2020;10.
IEEE
[1]
S. Gbashi, P. B. Njobeh, N. E. Madala, M. De Boevre, V. O. Kagot, and S. De Saeger, “Parallel validation of a green-solvent extraction method and quantitative estimation of multi-mycotoxins in staple cereals using LC-MS/MS,” SCIENTIFIC REPORTS, vol. 10, 2020.
@article{8673117,
  abstract     = {{In this study, 15 different mycotoxins were estimated in three staple cereals from selected agro-ecological regions in Nigeria using a 'novel' green extraction method, pressurized hot water extraction (PHWE) in comparison to a conventional solvent extraction method. Discrimination of the results of PHWE and solvent extraction using principal component analysis (PCA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA) did not yield any differential clustering patterns. All maize samples (n=16), 32% (n=38) of sorghum and 35% (n=37) of millet samples were positive for at least one of the 15 tested mycotoxins. Contamination levels for the cereals were higher in the warm humid rain forest region and gradually decreased towards the hot and arid region in the north of the country. The results demonstrate the applicability of PHWE as a possible alternative extraction method to conventional methods of extraction, which are solvent based.}},
  articleno    = {{10334}},
  author       = {{Gbashi, Sefater and Njobeh, Patrick Berka and Madala, Ntakadzeni Edwin and De Boevre, Marthe and Kagot, Victor Omondi and De Saeger, Sarah}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  keywords     = {{5 AGROECOLOGICAL ZONES,TANDEM MASS-SPECTROMETRY,FUMONISIN B1,OCHRATOXIN-A,AFLATOXIN CONTAMINATION,ALTERNARIA MYCOTOXINS,MAIZE,EXPOSURE,RISK,ZEARALENONE}},
  language     = {{eng}},
  pages        = {{16}},
  title        = {{Parallel validation of a green-solvent extraction method and quantitative estimation of multi-mycotoxins in staple cereals using LC-MS/MS}},
  url          = {{http://dx.doi.org/10.1038/s41598-020-66787-z}},
  volume       = {{10}},
  year         = {{2020}},
}

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