Advanced search
1 file | 3.05 MB Add to list

Extracellular vesicles in patients in the acute phase of psychosis and after clinical improvement : an explorative study

(2020) PEERJ. 8.
Author
Organization
Abstract
Extracellular vesicles (EVs) are cell-derived structures that transport proteins, lipids and nucleic acids between cells, thereby affecting the phenotype of the recipient cell. As the content of EVs reflects the status of the originating cell, EVs can have potential as biomarkers. Identifying EVs, including their cells of origin and their cargo, may provide insights in the pathophysiology of psychosis. Here, we present an in-depth analysis and proteomics of EVs from peripheral blood in patients (<jats:italic>n</jats:italic> = 25) during and after the acute phase of psychosis. Concentration and protein content of EVs in psychotic patients were twofold higher than in 25 age- and sex-matched healthy controls (<jats:italic>p</jats:italic> &lt; 0.001 for both concentration and protein content), and the diameter of EVs was larger in patients (<jats:italic>p</jats:italic> = 0.02). Properties of EVs did not differ significantly in blood sampled during and after the acute psychotic episode. Proteomic analyses on isolated EVs from individual patients revealed 1,853 proteins, whereof 45 were brain-elevated proteins. Of these, five proteins involved in regulation of plasticity of glutamatergic synapses were significantly different in psychotic patients compared to controls; neurogranin (NRGN), neuron-specific calcium-binding protein hippocalcin (HPCA), kalirin (KALRN), beta-adducin (ADD2) and ankyrin-2 (ANK2). To summarize, our results show that peripheral EVs in psychotic patients are different from those in healthy controls and point at alterations on the glutamatergic system. We suggest that EVs allow investigation of blood-borne brain-originating biological material and that their role as biomarkers in patients with psychotic disorders is worthy of further exploration.
Keywords
General Biochemistry, Genetics and Molecular Biology, General Neuroscience, General Agricultural and Biological Sciences, General Medicine, Psychosis, Proteomics, Extracellular vesicles, Gene ontology, Blood brain barrier, Glymphatics, Substance abuse, Immune system, Glutamatergic synapses, Brain enriched proteins, SUBSTANCE USE, SPECTRUM DISORDERS, BLOOD EXOSOMES, GAG PROTEIN, SCHIZOPHRENIA, PROTEOMICS, SYNAPSE, ABUSE, MICROPARTICLES, STABILITY

Downloads

  • TVDB peerj-9714.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 3.05 MB

Citation

Please use this url to cite or link to this publication:

MLA
Tunset, Mette Elise, et al. “Extracellular Vesicles in Patients in the Acute Phase of Psychosis and after Clinical Improvement : An Explorative Study.” PEERJ, vol. 8, 2020, doi:10.7717/peerj.9714.
APA
Tunset, M. E., Haslene-Hox, H., Van Den Bossche, T., Vaaler, A. E., Sulheim, E., & Kondziella, D. (2020). Extracellular vesicles in patients in the acute phase of psychosis and after clinical improvement : an explorative study. PEERJ, 8. https://doi.org/10.7717/peerj.9714
Chicago author-date
Tunset, Mette Elise, Hanne Haslene-Hox, Tim Van Den Bossche, Arne Einar Vaaler, Einar Sulheim, and Daniel Kondziella. 2020. “Extracellular Vesicles in Patients in the Acute Phase of Psychosis and after Clinical Improvement : An Explorative Study.” PEERJ 8. https://doi.org/10.7717/peerj.9714.
Chicago author-date (all authors)
Tunset, Mette Elise, Hanne Haslene-Hox, Tim Van Den Bossche, Arne Einar Vaaler, Einar Sulheim, and Daniel Kondziella. 2020. “Extracellular Vesicles in Patients in the Acute Phase of Psychosis and after Clinical Improvement : An Explorative Study.” PEERJ 8. doi:10.7717/peerj.9714.
Vancouver
1.
Tunset ME, Haslene-Hox H, Van Den Bossche T, Vaaler AE, Sulheim E, Kondziella D. Extracellular vesicles in patients in the acute phase of psychosis and after clinical improvement : an explorative study. PEERJ. 2020;8.
IEEE
[1]
M. E. Tunset, H. Haslene-Hox, T. Van Den Bossche, A. E. Vaaler, E. Sulheim, and D. Kondziella, “Extracellular vesicles in patients in the acute phase of psychosis and after clinical improvement : an explorative study,” PEERJ, vol. 8, 2020.
@article{8673055,
  abstract     = {Extracellular vesicles (EVs) are cell-derived structures that transport proteins, lipids and nucleic acids between cells, thereby affecting the phenotype of the recipient cell. As the content of EVs reflects the status of the originating cell, EVs can have potential as biomarkers. Identifying EVs, including their cells of origin and their cargo, may provide insights in the pathophysiology of psychosis. Here, we present an in-depth analysis and proteomics of EVs from peripheral blood in patients (<jats:italic>n</jats:italic> = 25) during and after the acute phase of psychosis. Concentration and protein content of EVs in psychotic patients were twofold higher than in 25 age- and sex-matched healthy controls (<jats:italic>p</jats:italic> &lt; 0.001 for both concentration and protein content), and the diameter of EVs was larger in patients (<jats:italic>p</jats:italic> = 0.02). Properties of EVs did not differ significantly in blood sampled during and after the acute psychotic episode. Proteomic analyses on isolated EVs from individual patients revealed 1,853 proteins, whereof 45 were brain-elevated proteins. Of these, five proteins involved in regulation of plasticity of glutamatergic synapses were significantly different in psychotic patients compared to controls; neurogranin (NRGN), neuron-specific calcium-binding protein hippocalcin (HPCA), kalirin (KALRN), beta-adducin (ADD2) and ankyrin-2 (ANK2). To summarize, our results show that peripheral EVs in psychotic patients are different from those in healthy controls and point at alterations on the glutamatergic system. We suggest that EVs allow investigation of blood-borne brain-originating biological material and that their role as biomarkers in patients with psychotic disorders is worthy of further exploration.},
  articleno    = {e9714},
  author       = {Tunset, Mette Elise and Haslene-Hox, Hanne and Van Den Bossche, Tim and Vaaler, Arne Einar and Sulheim, Einar and Kondziella, Daniel},
  issn         = {2167-8359},
  journal      = {PEERJ},
  keywords     = {General Biochemistry,Genetics and Molecular Biology,General Neuroscience,General Agricultural and Biological Sciences,General Medicine,Psychosis,Proteomics,Extracellular vesicles,Gene ontology,Blood brain barrier,Glymphatics,Substance abuse,Immune system,Glutamatergic synapses,Brain enriched proteins,SUBSTANCE USE,SPECTRUM DISORDERS,BLOOD EXOSOMES,GAG PROTEIN,SCHIZOPHRENIA,PROTEOMICS,SYNAPSE,ABUSE,MICROPARTICLES,STABILITY},
  language     = {eng},
  pages        = {29},
  title        = {Extracellular vesicles in patients in the acute phase of psychosis and after clinical improvement : an explorative study},
  url          = {http://dx.doi.org/10.7717/peerj.9714},
  volume       = {8},
  year         = {2020},
}

Altmetric
View in Altmetric
Web of Science
Times cited: