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Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm

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Abstract
The lungs of cystic fibrosis (CF) patients are often chronically colonized by multiple microbial species that can form biofilms, including the major CF pathogen Pseudomonas aeruginosa. Herewith, lower microbial diversity in CF airways is typically associated with worse health outcomes. In an attempt to treat CF lung infections patients are frequently exposed to antibiotics, which may affect microbial diversity. This study aimed at understanding if common antibiotics that target P. aeruginosa influence microbial diversity. To this end, a microaerophilic multispecies biofilm model of frequently co-isolated members of the CF lung microbiome (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus anginosus, Achromobacter xylosoxidans, Rothia mucilaginosa, and Gemella haemolysans) was exposed to antipseudomonal antibiotics. We found that antibiotics that affected several dominant species (i.e. ceftazidime, tobramycin) resulted in higher species evenness compared to colistin, which is only active against P. aeruginosa. Furthermore, susceptibility of individual species in the multispecies biofilm following antibiotic treatment was compared to that of the respective single-species biofilms, showing no differences. Adding three anaerobic species (Prevotella melaninogenica, Veillonella parvula, and Fusobacterium nucleatum) to the multispecies biofilm did not influence antibiotic susceptibility. In conclusion, our study demonstrates antibiotic-dependent effects on microbial community diversity of multispecies biofilms comprised of CF microbiome members.
Keywords
PSEUDOMONAS-AERUGINOSA, STAPHYLOCOCCUS-AUREUS, SYNERGISTIC INTERACTIONS, ANAEROBIC-BACTERIA, RESISTANCE, EXACERBATIONS, ENVIRONMENT, EXPRESSION, DIVERSITY, PATHOGENS, Multispecies biofilms, Antibiotics, Cystic fibrosis, Microbial diversity, Microbiota

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MLA
Vandeplassche, Eva, et al. “Antibiotic Susceptibility of Cystic Fibrosis Lung Microbiome Members in a Multispecies Biofilm.” BIOFILM (AMSTERDAM), vol. 2, 2020, doi:10.1016/j.bioflm.2020.100031.
APA
Vandeplassche, E., Sass, A., Ostyn, L., Burmølle, M., Kragh, K. N., Bjarnsholt, T., … Crabbé, A. (2020). Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm. BIOFILM (AMSTERDAM), 2. https://doi.org/10.1016/j.bioflm.2020.100031
Chicago author-date
Vandeplassche, Eva, Andrea Sass, Lisa Ostyn, Mette Burmølle, Kasper Nørskov Kragh, Thomas Bjarnsholt, Tom Coenye, and Aurélie Crabbé. 2020. “Antibiotic Susceptibility of Cystic Fibrosis Lung Microbiome Members in a Multispecies Biofilm.” BIOFILM (AMSTERDAM) 2. https://doi.org/10.1016/j.bioflm.2020.100031.
Chicago author-date (all authors)
Vandeplassche, Eva, Andrea Sass, Lisa Ostyn, Mette Burmølle, Kasper Nørskov Kragh, Thomas Bjarnsholt, Tom Coenye, and Aurélie Crabbé. 2020. “Antibiotic Susceptibility of Cystic Fibrosis Lung Microbiome Members in a Multispecies Biofilm.” BIOFILM (AMSTERDAM) 2. doi:10.1016/j.bioflm.2020.100031.
Vancouver
1.
Vandeplassche E, Sass A, Ostyn L, Burmølle M, Kragh KN, Bjarnsholt T, et al. Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm. BIOFILM (AMSTERDAM). 2020;2.
IEEE
[1]
E. Vandeplassche et al., “Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm,” BIOFILM (AMSTERDAM), vol. 2, 2020.
@article{8672973,
  abstract     = {{The lungs of cystic fibrosis (CF) patients are often chronically colonized by multiple microbial species that can form biofilms, including the major CF pathogen Pseudomonas aeruginosa. Herewith, lower microbial diversity in CF airways is typically associated with worse health outcomes. In an attempt to treat CF lung infections patients are frequently exposed to antibiotics, which may affect microbial diversity. This study aimed at understanding if common antibiotics that target P. aeruginosa influence microbial diversity. To this end, a microaerophilic multispecies biofilm model of frequently co-isolated members of the CF lung microbiome (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus anginosus, Achromobacter xylosoxidans, Rothia mucilaginosa, and Gemella haemolysans) was exposed to antipseudomonal antibiotics. We found that antibiotics that affected several dominant species (i.e. ceftazidime, tobramycin) resulted in higher species evenness compared to colistin, which is only active against P. aeruginosa. Furthermore, susceptibility of individual species in the multispecies biofilm following antibiotic treatment was compared to that of the respective single-species biofilms, showing no differences. Adding three anaerobic species (Prevotella melaninogenica, Veillonella parvula, and Fusobacterium nucleatum) to the multispecies biofilm did not influence antibiotic susceptibility. In conclusion, our study demonstrates antibiotic-dependent effects on microbial community diversity of multispecies biofilms comprised of CF microbiome members.}},
  articleno    = {{100031}},
  author       = {{Vandeplassche, Eva and Sass, Andrea and Ostyn, Lisa and Burmølle, Mette and Kragh, Kasper Nørskov and Bjarnsholt, Thomas and Coenye, Tom and Crabbé, Aurélie}},
  issn         = {{2590-2075}},
  journal      = {{BIOFILM (AMSTERDAM)}},
  keywords     = {{PSEUDOMONAS-AERUGINOSA,STAPHYLOCOCCUS-AUREUS,SYNERGISTIC INTERACTIONS,ANAEROBIC-BACTERIA,RESISTANCE,EXACERBATIONS,ENVIRONMENT,EXPRESSION,DIVERSITY,PATHOGENS,Multispecies biofilms,Antibiotics,Cystic fibrosis,Microbial diversity,Microbiota}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm}},
  url          = {{http://doi.org/10.1016/j.bioflm.2020.100031}},
  volume       = {{2}},
  year         = {{2020}},
}

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