Crossbreeding effect of double-muscled cattle on in vitro embryo development and quality
- Author
- Gretania Residiwati, Habib S A Tuska, Nima AzariDolatabad, Shehu Sidi, Petra Van Damme (UGent) , Krishna C Pavani, Osvaldo Américo Bogado Pascottini (UGent) , Geert Opsomer (UGent) and Ann Van Soom (UGent)
- Organization
- Abstract
- Nowadays, several developing countries have started to breed double-muscled cattle to their autochthonous cattle to improve meat production. However, the developmental competence of the resultant crossbreeding embryos is unknown. The objective of this study was to evaluate the effect of crossbreeding double-muscled (Belgian Blue; BB) semen with beef (Limousin; LIM) and dairy (Holstein-Friesian; HF) derived oocytes on embryo development and quality, using purebred BB as a control (BB oocytes fertilized by BB sperm). A single ejaculate of a BB bull was evaluated by Computer Assisted Sperm Analysis before using for in vitro fertilization. Ovaries from each breed were collected at the local slaughterhouse (n = 1,720 oocytes). All statistical analyses were performed using R-core (P < 0.05). Embryo quality was evaluated via differential-apoptotic staining of day 8 blastocysts. Cleavage (48 h post insemination) and day 8 blastocyst rates were greater (P < 0.05) for LIM (82.9 ± 6 and 27 ± 4.3%, respectively) than for BB (69.8 ± 8.5 and 19.6 ± 3.1%, respectively) and HF (45.1 ± 10 and 12.3 ± 2.2%, respectively). Holstein-Friesian presented lower cleavage and day 8 blastocyst rates than BB (P < 0.05). Limousin blastocysts presented a higher number (P < 0.05) of inner cell mass cells (ICM; 68 ± 7.8) than HF (40.4 ± 8.2). In conclusion, crossbreeding double-muscled cattle by in vitro fertilization with LIM oocytes yielded better embryo compared with the purebred combination, while the combination with HF oocytes produced the lowest rate of blastocysts.
- Keywords
- Belgian Blue, fertility, semen, oocyte
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8672076
- MLA
- Residiwati, Gretania, et al. “Crossbreeding Effect of Double-Muscled Cattle on in Vitro Embryo Development and Quality.” REPRODUCTIVE BIOLOGY, vol. 20, no. 3, 2020, pp. 288–92, doi:10.1016/j.repbio.2020.07.007.
- APA
- Residiwati, G., S A Tuska, H., AzariDolatabad, N., Sidi, S., Van Damme, P., C Pavani, K., … Van Soom, A. (2020). Crossbreeding effect of double-muscled cattle on in vitro embryo development and quality. REPRODUCTIVE BIOLOGY, 20(3), 288–292. https://doi.org/10.1016/j.repbio.2020.07.007
- Chicago author-date
- Residiwati, Gretania, Habib S A Tuska, Nima AzariDolatabad, Shehu Sidi, Petra Van Damme, Krishna C Pavani, Osvaldo Américo Bogado Pascottini, Geert Opsomer, and Ann Van Soom. 2020. “Crossbreeding Effect of Double-Muscled Cattle on in Vitro Embryo Development and Quality.” REPRODUCTIVE BIOLOGY 20 (3): 288–92. https://doi.org/10.1016/j.repbio.2020.07.007.
- Chicago author-date (all authors)
- Residiwati, Gretania, Habib S A Tuska, Nima AzariDolatabad, Shehu Sidi, Petra Van Damme, Krishna C Pavani, Osvaldo Américo Bogado Pascottini, Geert Opsomer, and Ann Van Soom. 2020. “Crossbreeding Effect of Double-Muscled Cattle on in Vitro Embryo Development and Quality.” REPRODUCTIVE BIOLOGY 20 (3): 288–292. doi:10.1016/j.repbio.2020.07.007.
- Vancouver
- 1.Residiwati G, S A Tuska H, AzariDolatabad N, Sidi S, Van Damme P, C Pavani K, et al. Crossbreeding effect of double-muscled cattle on in vitro embryo development and quality. REPRODUCTIVE BIOLOGY. 2020;20(3):288–92.
- IEEE
- [1]G. Residiwati et al., “Crossbreeding effect of double-muscled cattle on in vitro embryo development and quality,” REPRODUCTIVE BIOLOGY, vol. 20, no. 3, pp. 288–292, 2020.
@article{8672076,
abstract = {{Nowadays, several developing countries have started to breed double-muscled cattle to their autochthonous cattle to improve meat production. However, the developmental competence of the resultant crossbreeding embryos is unknown. The objective of this study was to evaluate the effect of crossbreeding double-muscled (Belgian Blue; BB) semen with beef (Limousin; LIM) and dairy (Holstein-Friesian; HF) derived oocytes on embryo development and quality, using purebred BB as a control (BB oocytes fertilized by BB sperm). A single ejaculate of a BB bull was evaluated by Computer Assisted Sperm Analysis before using for in vitro fertilization. Ovaries from each breed were collected at the local slaughterhouse (n = 1,720 oocytes). All statistical analyses were performed using R-core (P < 0.05). Embryo quality was evaluated via differential-apoptotic staining of day 8 blastocysts. Cleavage (48 h post insemination) and day 8 blastocyst rates were greater (P < 0.05) for LIM (82.9 ± 6 and 27 ± 4.3%, respectively) than for BB (69.8 ± 8.5 and 19.6 ± 3.1%, respectively) and HF (45.1 ± 10 and 12.3 ± 2.2%, respectively). Holstein-Friesian presented lower cleavage and day 8 blastocyst rates than BB (P < 0.05). Limousin blastocysts presented a higher number (P < 0.05) of inner cell mass cells (ICM; 68 ± 7.8) than HF (40.4 ± 8.2). In conclusion, crossbreeding double-muscled cattle by in vitro fertilization with LIM oocytes yielded better embryo compared with the purebred combination, while the combination with HF oocytes produced the lowest rate of blastocysts.}},
author = {{Residiwati, Gretania and S A Tuska, Habib and AzariDolatabad, Nima and Sidi, Shehu and Van Damme, Petra and C Pavani, Krishna and Bogado Pascottini, Osvaldo Américo and Opsomer, Geert and Van Soom, Ann}},
issn = {{1642-431X}},
journal = {{REPRODUCTIVE BIOLOGY}},
keywords = {{Belgian Blue,fertility,semen,oocyte}},
language = {{eng}},
number = {{3}},
pages = {{288--292}},
title = {{Crossbreeding effect of double-muscled cattle on in vitro embryo development and quality}},
url = {{http://doi.org/10.1016/j.repbio.2020.07.007}},
volume = {{20}},
year = {{2020}},
}
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