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Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates

Lore Dewanckele, Jeyamalar Jeyanathan (UGent) , Bruno Vlaeminck (UGent) and Veerle Fievez (UGent)
(2020) BMC MICROBIOLOGY. 20(1).
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Abstract
Background Bacteria involved in ruminal formation oftrans-10 intermediates are unclear. Therefore, this study aimed at identifying rumen bacteria that producetrans-10 intermediates from 18-carbon unsaturated fatty acids. Results Pure cultures of 28 rumen bacterial species were incubated individually in the presence of 40 mu g/mL 18:3n-3, 18:2n-6 ortrans-11 18:1 under control or lactate-enriched (200 mM Na lactate) conditions for 24 h. Of the 28 strains,Cutibacterium acnes(formerlyPropionibacterium acnes) was the only bacterium found to producetrans-10 intermediates from 18:3n-3 and 18:2n-6, irrespective of the growth condition. To further assess the potential importance of this species in thetrans-11 totrans-10 shift, different biomass ratios ofButyrivibrio fibrisolvens(as atrans-11 producer) andC. acneswere incubated in different growth media (control, low pH and 22:6n-3 enriched media) containing 40 mu g/mL 18:2n-6. Under control conditions, atrans-10 shift, defined in the current study astrans-10/trans-11 >= 0.9, occurred when the biomass ofC. acnesrepresented between 90 and 98% of the inoculum. A low pH or addition of 22:6n-3 inhibitedcis-9,trans-11 CLA andtrans-10,cis-12 CLA formation byB. fibrisolvensandC. acnes, respectively, wherebyC. acnesseemed to be more tolerant. This resulted in a decreased biomass ofC. acnesrequired at inoculation to induce atrans-10 shift to 50% (low pH) and 90% (22:6n-3 addition). Conclusions Among the bacterial species studied,C. acneswas the only bacterium that have the metabolic ability to producetrans-10 intermediates from 18:3n-3 and 18:2n-6. Nevertheless, this experiment revealed that it is unlikely thatC. acnesis the only or predominant species involved in thetrans-11 totrans-10 shift in vivo.
Keywords
Microbiology (medical), Microbiology, Biohydrogenation, Cuitbacterium acnes, Pure cultures, Rumen, trans-11 totrans-10 shift, CONJUGATED LINOLEIC-ACID, SUBACUTE RUMINAL ACIDOSIS, UNSATURATED FATTY-ACIDS, MILK-FAT, DAIRY-COWS, NUTRITIONAL REGULATION, COMMUNITY COMPOSITION, LIPID-METABOLISM, OIL, PRODUCTS

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MLA
Dewanckele, Lore, et al. “Identifying and Exploring Biohydrogenating Rumen Bacteria with Emphasis on Pathways Including Trans-10 Intermediates.” BMC MICROBIOLOGY, vol. 20, no. 1, 2020, doi:10.1186/s12866-020-01876-7.
APA
Dewanckele, L., Jeyanathan, J., Vlaeminck, B., & Fievez, V. (2020). Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates. BMC MICROBIOLOGY, 20(1). https://doi.org/10.1186/s12866-020-01876-7
Chicago author-date
Dewanckele, Lore, Jeyamalar Jeyanathan, Bruno Vlaeminck, and Veerle Fievez. 2020. “Identifying and Exploring Biohydrogenating Rumen Bacteria with Emphasis on Pathways Including Trans-10 Intermediates.” BMC MICROBIOLOGY 20 (1). https://doi.org/10.1186/s12866-020-01876-7.
Chicago author-date (all authors)
Dewanckele, Lore, Jeyamalar Jeyanathan, Bruno Vlaeminck, and Veerle Fievez. 2020. “Identifying and Exploring Biohydrogenating Rumen Bacteria with Emphasis on Pathways Including Trans-10 Intermediates.” BMC MICROBIOLOGY 20 (1). doi:10.1186/s12866-020-01876-7.
Vancouver
1.
Dewanckele L, Jeyanathan J, Vlaeminck B, Fievez V. Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates. BMC MICROBIOLOGY. 2020;20(1).
IEEE
[1]
L. Dewanckele, J. Jeyanathan, B. Vlaeminck, and V. Fievez, “Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates,” BMC MICROBIOLOGY, vol. 20, no. 1, 2020.
@article{8671597,
  abstract     = {{Background Bacteria involved in ruminal formation oftrans-10 intermediates are unclear. Therefore, this study aimed at identifying rumen bacteria that producetrans-10 intermediates from 18-carbon unsaturated fatty acids. Results Pure cultures of 28 rumen bacterial species were incubated individually in the presence of 40 mu g/mL 18:3n-3, 18:2n-6 ortrans-11 18:1 under control or lactate-enriched (200 mM Na lactate) conditions for 24 h. Of the 28 strains,Cutibacterium acnes(formerlyPropionibacterium acnes) was the only bacterium found to producetrans-10 intermediates from 18:3n-3 and 18:2n-6, irrespective of the growth condition. To further assess the potential importance of this species in thetrans-11 totrans-10 shift, different biomass ratios ofButyrivibrio fibrisolvens(as atrans-11 producer) andC. acneswere incubated in different growth media (control, low pH and 22:6n-3 enriched media) containing 40 mu g/mL 18:2n-6. Under control conditions, atrans-10 shift, defined in the current study astrans-10/trans-11 >= 0.9, occurred when the biomass ofC. acnesrepresented between 90 and 98% of the inoculum. A low pH or addition of 22:6n-3 inhibitedcis-9,trans-11 CLA andtrans-10,cis-12 CLA formation byB. fibrisolvensandC. acnes, respectively, wherebyC. acnesseemed to be more tolerant. This resulted in a decreased biomass ofC. acnesrequired at inoculation to induce atrans-10 shift to 50% (low pH) and 90% (22:6n-3 addition). Conclusions Among the bacterial species studied,C. acneswas the only bacterium that have the metabolic ability to producetrans-10 intermediates from 18:3n-3 and 18:2n-6. Nevertheless, this experiment revealed that it is unlikely thatC. acnesis the only or predominant species involved in thetrans-11 totrans-10 shift in vivo.}},
  articleno    = {{198}},
  author       = {{Dewanckele, Lore and Jeyanathan, Jeyamalar and Vlaeminck, Bruno and Fievez, Veerle}},
  issn         = {{1471-2180}},
  journal      = {{BMC MICROBIOLOGY}},
  keywords     = {{Microbiology (medical),Microbiology,Biohydrogenation,Cuitbacterium acnes,Pure cultures,Rumen,trans-11 totrans-10 shift,CONJUGATED LINOLEIC-ACID,SUBACUTE RUMINAL ACIDOSIS,UNSATURATED FATTY-ACIDS,MILK-FAT,DAIRY-COWS,NUTRITIONAL REGULATION,COMMUNITY COMPOSITION,LIPID-METABOLISM,OIL,PRODUCTS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{21}},
  title        = {{Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates}},
  url          = {{http://doi.org/10.1186/s12866-020-01876-7}},
  volume       = {{20}},
  year         = {{2020}},
}
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