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Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids

Nina Turk (UGent) , Ali Raza (UGent) , Pieter Wuytens (UGent) , Hans Demol (UGent) , Michiel Van Daele (UGent) , Christophe Detavernier (UGent) , Andre Skirtach (UGent) , Kris Gevaert (UGent) and Roel Baets (UGent)
(2020) BIOMEDICAL OPTICS EXPRESS. 11(8). p.4800-4816
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Abstract
Surface enhanced Raman spectroscopy (SERS) is a selective and sensitive technique, which allows for the detection of protease activity by monitoring the cleavage of peptide substrates. Commonly used free-space based SERS substrates, however, require the use of bulky and expensive instrumentation, limiting their use to laboratory environments. An integrated photonics approach aims to implement various free-space optical components to a reliable, mass-reproducible and cheap photonic chip. We here demonstrate integrated SERS detection of trypsin activity using a nanoplasmonic slot waveguide as a waveguide-based SERS substrate. Despite the continuously improving SERS performance of the waveguide-based SERS substrates, they currently still do not reach the SERS enhancements of free-space substrates. To mitigate this, we developed an improved peptide substrate in which we incorporated the non-natural aromatic amino acid 4-cyano-phenylalanine, which provides a high intrinsic SERS signal. The use of non-natural aromatics is expected to extend the possibilities for multiplexing measurements, where the activity of several proteases can be detected simultaneously.
Keywords
Biotechnology, Atomic and Molecular Physics, and Optics

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MLA
Turk, Nina, et al. “Waveguide-Based Surface-Enhanced Raman Spectroscopy Detection of Protease Activity Using Non-Natural Aromatic Amino Acids.” BIOMEDICAL OPTICS EXPRESS, vol. 11, no. 8, 2020, pp. 4800–16, doi:10.1364/boe.398038.
APA
Turk, N., Raza, A., Wuytens, P., Demol, H., Van Daele, M., Detavernier, C., … Baets, R. (2020). Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids. BIOMEDICAL OPTICS EXPRESS, 11(8), 4800–4816. https://doi.org/10.1364/boe.398038
Chicago author-date
Turk, Nina, Ali Raza, Pieter Wuytens, Hans Demol, Michiel Van Daele, Christophe Detavernier, Andre Skirtach, Kris Gevaert, and Roel Baets. 2020. “Waveguide-Based Surface-Enhanced Raman Spectroscopy Detection of Protease Activity Using Non-Natural Aromatic Amino Acids.” BIOMEDICAL OPTICS EXPRESS 11 (8): 4800–4816. https://doi.org/10.1364/boe.398038.
Chicago author-date (all authors)
Turk, Nina, Ali Raza, Pieter Wuytens, Hans Demol, Michiel Van Daele, Christophe Detavernier, Andre Skirtach, Kris Gevaert, and Roel Baets. 2020. “Waveguide-Based Surface-Enhanced Raman Spectroscopy Detection of Protease Activity Using Non-Natural Aromatic Amino Acids.” BIOMEDICAL OPTICS EXPRESS 11 (8): 4800–4816. doi:10.1364/boe.398038.
Vancouver
1.
Turk N, Raza A, Wuytens P, Demol H, Van Daele M, Detavernier C, et al. Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids. BIOMEDICAL OPTICS EXPRESS. 2020;11(8):4800–16.
IEEE
[1]
N. Turk et al., “Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids,” BIOMEDICAL OPTICS EXPRESS, vol. 11, no. 8, pp. 4800–4816, 2020.
@article{8671171,
  abstract     = {Surface enhanced Raman spectroscopy (SERS) is a selective and sensitive technique, which allows for the detection of protease activity by monitoring the cleavage of peptide substrates. Commonly used free-space based SERS substrates, however, require the use of bulky and expensive instrumentation, limiting their use to laboratory environments. An integrated photonics approach aims to implement various free-space optical components to a reliable, mass-reproducible and cheap photonic chip. We here demonstrate integrated SERS detection of trypsin activity using a nanoplasmonic slot waveguide as a waveguide-based SERS substrate. Despite the continuously improving SERS performance of the waveguide-based SERS substrates, they currently still do not reach the SERS enhancements of free-space substrates. To mitigate this, we developed an improved peptide substrate in which we incorporated the non-natural aromatic amino acid 4-cyano-phenylalanine, which provides a high intrinsic SERS signal. The use of non-natural aromatics is expected to extend the possibilities for multiplexing measurements, where the activity of several proteases can be detected simultaneously.},
  author       = {Turk, Nina and Raza, Ali and Wuytens, Pieter and Demol, Hans and Van Daele, Michiel and Detavernier, Christophe and Skirtach, Andre and Gevaert, Kris and Baets, Roel},
  issn         = {2156-7085},
  journal      = {BIOMEDICAL OPTICS EXPRESS},
  keywords     = {Biotechnology,Atomic and Molecular Physics,and Optics},
  language     = {eng},
  number       = {8},
  pages        = {4800--4816},
  title        = {Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids},
  url          = {http://dx.doi.org/10.1364/boe.398038},
  volume       = {11},
  year         = {2020},
}

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