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An unbiased immunization strategy results in the identification of enolase as a potential marker for nanobody-based detection of Trypanosoma evansi

(2020) VACCINES. 8(3).
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Abstract
Trypanosoma evansi is a widely spread parasite that causes the debilitating disease “surra” in several types of ungulates. This severely challenges livestock rearing and heavily weighs on the socio-economic development in the affected areas, which include countries on five continents. Active case finding requires a sensitive and specific diagnostic test. In this paper, we describe the application of an unbiased immunization strategy to identify potential biomarkers for Nanobody (Nb)-based detection of T. evansi infections. Alpaca immunization with soluble lysates from different T. evansi strains followed by panning against T. evansi secretome resulted in the selection of a single Nb (Nb11). By combining Nb11-mediated immuno-capturing with mass spectrometry, the T. evansi target antigen was identified as the glycolytic enzyme enolase. Four additional anti-enolase binders were subsequently generated by immunizing another alpaca with the recombinant target enzyme. Together with Nb11, these binders were evaluated for their potential use in a heterologous sandwich detection format. Three Nb pairs were identified as candidates for the further development of an antigen-based assay for Nb-mediated diagnosis of T. evansi infection.
Keywords
Immunology, Pharmacology (medical), Pharmacology, Drug Discovery, Infectious Diseases

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MLA
Li, Zeng, et al. “An Unbiased Immunization Strategy Results in the Identification of Enolase as a Potential Marker for Nanobody-Based Detection of Trypanosoma Evansi.” VACCINES, vol. 8, no. 3, 2020, doi:10.3390/vaccines8030415.
APA
Li, Z., Pinto Torres, J. E., Goossens, J., Vertommen, D., Caljon, G., Sterckx, Y. G.-J., & Magez, S. (2020). An unbiased immunization strategy results in the identification of enolase as a potential marker for nanobody-based detection of Trypanosoma evansi. VACCINES, 8(3). https://doi.org/10.3390/vaccines8030415
Chicago author-date
Li, Zeng, Joar Esteban Pinto Torres, Julie Goossens, Didier Vertommen, Guy Caljon, Yann G. -J. Sterckx, and Stefan Magez. 2020. “An Unbiased Immunization Strategy Results in the Identification of Enolase as a Potential Marker for Nanobody-Based Detection of Trypanosoma Evansi.” VACCINES 8 (3). https://doi.org/10.3390/vaccines8030415.
Chicago author-date (all authors)
Li, Zeng, Joar Esteban Pinto Torres, Julie Goossens, Didier Vertommen, Guy Caljon, Yann G. -J. Sterckx, and Stefan Magez. 2020. “An Unbiased Immunization Strategy Results in the Identification of Enolase as a Potential Marker for Nanobody-Based Detection of Trypanosoma Evansi.” VACCINES 8 (3). doi:10.3390/vaccines8030415.
Vancouver
1.
Li Z, Pinto Torres JE, Goossens J, Vertommen D, Caljon G, Sterckx YG-J, et al. An unbiased immunization strategy results in the identification of enolase as a potential marker for nanobody-based detection of Trypanosoma evansi. VACCINES. 2020;8(3).
IEEE
[1]
Z. Li et al., “An unbiased immunization strategy results in the identification of enolase as a potential marker for nanobody-based detection of Trypanosoma evansi,” VACCINES, vol. 8, no. 3, 2020.
@article{8671129,
  abstract     = {Trypanosoma evansi is a widely spread parasite that causes the debilitating disease “surra” in several types of ungulates. This severely challenges livestock rearing and heavily weighs on the socio-economic development in the affected areas, which include countries on five continents. Active case finding requires a sensitive and specific diagnostic test. In this paper, we describe the application of an unbiased immunization strategy to identify potential biomarkers for Nanobody (Nb)-based detection of T. evansi infections. Alpaca immunization with soluble lysates from different T. evansi strains followed by panning against T. evansi secretome resulted in the selection of a single Nb (Nb11). By combining Nb11-mediated immuno-capturing with mass spectrometry, the T. evansi target antigen was identified as the glycolytic enzyme enolase. Four additional anti-enolase binders were subsequently generated by immunizing another alpaca with the recombinant target enzyme. Together with Nb11, these binders were evaluated for their potential use in a heterologous sandwich detection format. Three Nb pairs were identified as candidates for the further development of an antigen-based assay for Nb-mediated diagnosis of T. evansi infection.},
  articleno    = {415},
  author       = {Li, Zeng and Pinto Torres, Joar Esteban and Goossens, Julie and Vertommen, Didier and Caljon, Guy and Sterckx, Yann G. -J. and Magez, Stefan},
  issn         = {2076-393X},
  journal      = {VACCINES},
  keywords     = {Immunology,Pharmacology (medical),Pharmacology,Drug Discovery,Infectious Diseases},
  language     = {eng},
  number       = {3},
  pages        = {19},
  title        = {An unbiased immunization strategy results in the identification of enolase as a potential marker for nanobody-based detection of Trypanosoma evansi},
  url          = {http://dx.doi.org/10.3390/vaccines8030415},
  volume       = {8},
  year         = {2020},
}

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