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Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development

Juliette Roels (UGent) , Anna Kuchmiy (UGent) , Matthias De Decker (UGent) , Steven Strubbe (UGent) , Marieke Lavaert (UGent) , Kai Ling Liang (UGent) , Georges Leclercq (UGent) , Bart Vandekerckhove (UGent) , Filip Van Nieuwerburgh (UGent) , Pieter Van Vlierberghe (UGent) , et al.
(2020) NATURE IMMUNOLOGY. 21. p.1280-1292
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Abstract
The epigenetic landscape of human alpha beta and gamma delta T cell development has remained unexplored thus far. Taghon and colleagues provide a resource of RNA-seq and ATAC-seq profiles examining human thymocyte development. The development of TCR alpha beta and TCR gamma delta T cells comprises a step-wise process in which regulatory events control differentiation and lineage outcome. To clarify these mechanisms, we employed RNA-sequencing, ATAC-sequencing and ChIPmentation on well-defined thymocyte subsets that represent the continuum of human T cell development. The chromatin accessibility dynamics show clear stage specificity and reveal that human T cell-lineage commitment is marked byGATA3- andBCL11B-dependent closing of PU.1 sites. A temporary increase in H3K27me3 without open chromatin modifications is unique for beta-selection, whereas emerging gamma delta T cells, which originate from common precursors of beta-selected cells, show large chromatin accessibility changes due to strong T cell receptor (TCR) signaling. Furthermore, we unravel distinct chromatin landscapes between CD4(+)and CD8(+)alpha beta-lineage cells that support their effector functions and reveal gene-specific mechanisms that define mature T cells. This resource provides a framework for studying gene regulatory mechanisms that drive normal and malignant human T cell development.
Keywords
Immunology, HELIX PROTEIN ID3, LINEAGE COMMITMENT, CHIP-SEQ, GENE-EXPRESSION, B-CELL, PROGENITORS, PRECURSORS, NETWORKS, MOUSE

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MLA
Roels, Juliette, et al. “Distinct and Temporary-Restricted Epigenetic Mechanisms Regulate Human Αβ and Γδ T Cell Development.” NATURE IMMUNOLOGY, vol. 21, 2020, pp. 1280–92, doi:10.1038/s41590-020-0747-9.
APA
Roels, J., Kuchmiy, A., De Decker, M., Strubbe, S., Lavaert, M., Liang, K. L., … Taghon, T. (2020). Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development. NATURE IMMUNOLOGY, 21, 1280–1292. https://doi.org/10.1038/s41590-020-0747-9
Chicago author-date
Roels, Juliette, Anna Kuchmiy, Matthias De Decker, Steven Strubbe, Marieke Lavaert, Kai Ling Liang, Georges Leclercq, et al. 2020. “Distinct and Temporary-Restricted Epigenetic Mechanisms Regulate Human Αβ and Γδ T Cell Development.” NATURE IMMUNOLOGY 21: 1280–92. https://doi.org/10.1038/s41590-020-0747-9.
Chicago author-date (all authors)
Roels, Juliette, Anna Kuchmiy, Matthias De Decker, Steven Strubbe, Marieke Lavaert, Kai Ling Liang, Georges Leclercq, Bart Vandekerckhove, Filip Van Nieuwerburgh, Pieter Van Vlierberghe, and Tom Taghon. 2020. “Distinct and Temporary-Restricted Epigenetic Mechanisms Regulate Human Αβ and Γδ T Cell Development.” NATURE IMMUNOLOGY 21: 1280–1292. doi:10.1038/s41590-020-0747-9.
Vancouver
1.
Roels J, Kuchmiy A, De Decker M, Strubbe S, Lavaert M, Liang KL, et al. Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development. NATURE IMMUNOLOGY. 2020;21:1280–92.
IEEE
[1]
J. Roels et al., “Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development,” NATURE IMMUNOLOGY, vol. 21, pp. 1280–1292, 2020.
@article{8671014,
  abstract     = {{The epigenetic landscape of human alpha beta and gamma delta T cell development has remained unexplored thus far. Taghon and colleagues provide a resource of RNA-seq and ATAC-seq profiles examining human thymocyte development.

The development of TCR alpha beta and TCR gamma delta T cells comprises a step-wise process in which regulatory events control differentiation and lineage outcome. To clarify these mechanisms, we employed RNA-sequencing, ATAC-sequencing and ChIPmentation on well-defined thymocyte subsets that represent the continuum of human T cell development. The chromatin accessibility dynamics show clear stage specificity and reveal that human T cell-lineage commitment is marked byGATA3- andBCL11B-dependent closing of PU.1 sites. A temporary increase in H3K27me3 without open chromatin modifications is unique for beta-selection, whereas emerging gamma delta T cells, which originate from common precursors of beta-selected cells, show large chromatin accessibility changes due to strong T cell receptor (TCR) signaling. Furthermore, we unravel distinct chromatin landscapes between CD4(+)and CD8(+)alpha beta-lineage cells that support their effector functions and reveal gene-specific mechanisms that define mature T cells. This resource provides a framework for studying gene regulatory mechanisms that drive normal and malignant human T cell development.}},
  author       = {{Roels, Juliette and Kuchmiy, Anna and De Decker, Matthias and Strubbe, Steven and Lavaert, Marieke and Liang, Kai Ling and Leclercq, Georges and Vandekerckhove, Bart and Van Nieuwerburgh, Filip and Van Vlierberghe, Pieter and Taghon, Tom}},
  issn         = {{1529-2908}},
  journal      = {{NATURE IMMUNOLOGY}},
  keywords     = {{Immunology,HELIX PROTEIN ID3,LINEAGE COMMITMENT,CHIP-SEQ,GENE-EXPRESSION,B-CELL,PROGENITORS,PRECURSORS,NETWORKS,MOUSE}},
  language     = {{eng}},
  pages        = {{1280--1292}},
  title        = {{Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development}},
  url          = {{http://dx.doi.org/10.1038/s41590-020-0747-9}},
  volume       = {{21}},
  year         = {{2020}},
}

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