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Comprehensive and functional analysis of horizontal gene transfer events in diatoms

(2020) MOLECULAR BIOLOGY AND EVOLUTION. 37(11). p.3243-3257
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Abstract
Diatoms are a diverse group of mainly photosynthetic algae, responsible for 20% of worldwide oxygen production, which can rapidly respond to favourable conditions and often outcompete other phytoplankton. We investigated the contribution of horizontal gene transfer (HGT) to its ecological success. A large-scale phylogeny-based prokaryotic HGT detection procedure across nine sequenced diatoms showed that 3-5% of their proteome has a horizontal origin and a large influx occurred at the ancestor of diatoms. More than 90% of HGT genes are expressed, and species-specific HGT genes in Phaeodactylum tricornutum undergo strong purifying selection. Genes derived from HGT are implicated in several processes including environmental sensing, and expand the metabolic toolbox. Cobalamin (vitamin B12) is an essential cofactor for roughly half of the diatoms and is only produced by bacteria. Five consecutive genes involved in the final synthesis of the cobalamin biosynthetic pathway, which could function as scavenging and repair genes, were detected as HGT. The full suite of these genes were detected in the cold-adapted diatom Fragilariopsis cylindrus. This might give diatoms originating from the Southern Ocean, a region typically depleted in cobalamin, a competitive advantage. Overall, we show that HGT is a prevalent mechanism that is actively used in diatoms to expand its adaptive capabilities.
Keywords
Genetics, Ecology, Evolution, Behavior and Systematics, Molecular Biology, horizontal gene transfer, diatoms, vitamin B12, selection pressure, MULTIPLE SEQUENCE ALIGNMENT, SYNEDRA-ACUS, GENOME, EVOLUTION, REVEAL, IRON, VITAMIN-B-12, ALGAE, EXPRESSION, FRAMEWORK

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Citation

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MLA
Vancaester, Emmelien, et al. “Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms.” MOLECULAR BIOLOGY AND EVOLUTION, vol. 37, no. 11, 2020, pp. 3243–57, doi:10.1093/molbev/msaa182.
APA
Vancaester, E., Depuydt, T., Osuna, C., & Vandepoele, K. (2020). Comprehensive and functional analysis of horizontal gene transfer events in diatoms. MOLECULAR BIOLOGY AND EVOLUTION, 37(11), 3243–3257. https://doi.org/10.1093/molbev/msaa182
Chicago author-date
Vancaester, Emmelien, Thomas Depuydt, Cristina Osuna, and Klaas Vandepoele. 2020. “Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms.” MOLECULAR BIOLOGY AND EVOLUTION 37 (11): 3243–57. https://doi.org/10.1093/molbev/msaa182.
Chicago author-date (all authors)
Vancaester, Emmelien, Thomas Depuydt, Cristina Osuna, and Klaas Vandepoele. 2020. “Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms.” MOLECULAR BIOLOGY AND EVOLUTION 37 (11): 3243–3257. doi:10.1093/molbev/msaa182.
Vancouver
1.
Vancaester E, Depuydt T, Osuna C, Vandepoele K. Comprehensive and functional analysis of horizontal gene transfer events in diatoms. MOLECULAR BIOLOGY AND EVOLUTION. 2020;37(11):3243–57.
IEEE
[1]
E. Vancaester, T. Depuydt, C. Osuna, and K. Vandepoele, “Comprehensive and functional analysis of horizontal gene transfer events in diatoms,” MOLECULAR BIOLOGY AND EVOLUTION, vol. 37, no. 11, pp. 3243–3257, 2020.
@article{8670450,
  abstract     = {{Diatoms are a diverse group of mainly photosynthetic algae, responsible for 20% of worldwide oxygen production, which can rapidly respond to favourable conditions and often outcompete other phytoplankton. We investigated the contribution of horizontal gene transfer (HGT) to its ecological success. A large-scale phylogeny-based prokaryotic HGT detection procedure across nine sequenced diatoms showed that 3-5% of their proteome has a horizontal origin and a large influx occurred at the ancestor of diatoms. More than 90% of HGT genes are expressed, and species-specific HGT genes in Phaeodactylum tricornutum undergo strong purifying selection. Genes derived from HGT are implicated in several processes including environmental sensing, and expand the metabolic toolbox. Cobalamin (vitamin B12) is an essential cofactor for roughly half of the diatoms and is only produced by bacteria. Five consecutive genes involved in the final synthesis of the cobalamin biosynthetic pathway, which could function as scavenging and repair genes, were detected as HGT. The full suite of these genes were detected in the cold-adapted diatom Fragilariopsis cylindrus. This might give diatoms originating from the Southern Ocean, a region typically depleted in cobalamin, a competitive advantage. Overall, we show that HGT is a prevalent mechanism that is actively used in diatoms to expand its adaptive capabilities.}},
  author       = {{Vancaester, Emmelien and Depuydt, Thomas and Osuna, Cristina and Vandepoele, Klaas}},
  issn         = {{0737-4038}},
  journal      = {{MOLECULAR BIOLOGY AND EVOLUTION}},
  keywords     = {{Genetics,Ecology,Evolution,Behavior and Systematics,Molecular Biology,horizontal gene transfer,diatoms,vitamin B12,selection pressure,MULTIPLE SEQUENCE ALIGNMENT,SYNEDRA-ACUS,GENOME,EVOLUTION,REVEAL,IRON,VITAMIN-B-12,ALGAE,EXPRESSION,FRAMEWORK}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{3243--3257}},
  title        = {{Comprehensive and functional analysis of horizontal gene transfer events in diatoms}},
  url          = {{http://dx.doi.org/10.1093/molbev/msaa182}},
  volume       = {{37}},
  year         = {{2020}},
}

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