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Searching for new long‐term urinary metabolites of metenolone and drostanolone using gas chromatography : mass spectrometry with a focus on non‐hydrolysed sulfates

(2020) DRUG TESTING AND ANALYSIS. 12(8). p.1041-1053
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Abstract
Sulfated metabolites have been shown to have potential as long-term markers of anabolic-androgenic steroid (AAS) abuse. In 2019, the compatibility of gas chromatography-mass spectrometry (GC-MS) with non-hydrolysed sulfated steroids was demonstrated, and this approach allowed the incorporation of these compounds in a broad GC-MS initial testing procedure at a later stage. However, research is needed to identify which are beneficial. In this study, a search for new long-term metabolites of two popular AAS, metenolone and drostanolone, was undertaken through two excretion studies each. The excretion samples were analysed using GC-chemical ionization-triple quadrupole MS (GC-CI-MS/MS) after the application of three separate sample preparation methodologies (i.e. hydrolysis withEscherichia coli-derived beta-glucuronidase,Helix pomatia-derived beta-glucuronidase/arylsulfatase and non-hydrolysed sulfated steroids). For metenolone, a non-hydrolysed sulfated metabolite, 1 beta-methyl-5 alpha-androstan-17-one-3 zeta-sulfate, was documented for the first time to provide the longest detection time of up to 17 days. This metabolite increased the detection time by nearly a factor of 2 in comparison with the currently monitored markers for metenolone in a routine doping control initial testing procedure. In the second excretion study, it prolonged the detection window by 25%. In the case of drostanolone, the non-hydrolysed sulfated metabolite with the longest detection time was the sulfated analogue of the main drostanolone metabolite (3 alpha-hydroxy-2 alpha-methyl-5 alpha-androstan-17-one) with a detection time of up to 24 days. However, the currently monitored main drostanolone metabolite in routine doping control, after hydrolysis of the glucuronide withE.coli, remained superior in detection time (i.e. up to 29 days).
Keywords
Analytical Chemistry, Spectroscopy, Pharmaceutical Science, Environmental Chemistry, drostanolone, GC-MS, metenolone, steroids, sulfates, METHENOLONE ACETATE PRIMOBOLAN, ANABOLIC-STEROIDS, DOPING CONTROL, IDENTIFICATION, MESTEROLONE, SENSITIVITY, IONIZATION, VALIDATION, MISUSE

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MLA
Albertsdóttir, Aðalheiður Dóra, et al. “Searching for New Long‐term Urinary Metabolites of Metenolone and Drostanolone Using Gas Chromatography : Mass Spectrometry with a Focus on Non‐hydrolysed Sulfates.” DRUG TESTING AND ANALYSIS, vol. 12, no. 8, 2020, pp. 1041–53, doi:10.1002/dta.2818.
APA
Albertsdóttir, A. D., Van Gansbeke, W., Coppieters, G., Balgimbekova, K., Van Eenoo, P., & Polet, M. (2020). Searching for new long‐term urinary metabolites of metenolone and drostanolone using gas chromatography : mass spectrometry with a focus on non‐hydrolysed sulfates. DRUG TESTING AND ANALYSIS, 12(8), 1041–1053. https://doi.org/10.1002/dta.2818
Chicago author-date
Albertsdóttir, Aðalheiður Dóra, Wim Van Gansbeke, Gilles Coppieters, Kyzylkul Balgimbekova, Peter Van Eenoo, and Michaël Polet. 2020. “Searching for New Long‐term Urinary Metabolites of Metenolone and Drostanolone Using Gas Chromatography : Mass Spectrometry with a Focus on Non‐hydrolysed Sulfates.” DRUG TESTING AND ANALYSIS 12 (8): 1041–53. https://doi.org/10.1002/dta.2818.
Chicago author-date (all authors)
Albertsdóttir, Aðalheiður Dóra, Wim Van Gansbeke, Gilles Coppieters, Kyzylkul Balgimbekova, Peter Van Eenoo, and Michaël Polet. 2020. “Searching for New Long‐term Urinary Metabolites of Metenolone and Drostanolone Using Gas Chromatography : Mass Spectrometry with a Focus on Non‐hydrolysed Sulfates.” DRUG TESTING AND ANALYSIS 12 (8): 1041–1053. doi:10.1002/dta.2818.
Vancouver
1.
Albertsdóttir AD, Van Gansbeke W, Coppieters G, Balgimbekova K, Van Eenoo P, Polet M. Searching for new long‐term urinary metabolites of metenolone and drostanolone using gas chromatography : mass spectrometry with a focus on non‐hydrolysed sulfates. DRUG TESTING AND ANALYSIS. 2020;12(8):1041–53.
IEEE
[1]
A. D. Albertsdóttir, W. Van Gansbeke, G. Coppieters, K. Balgimbekova, P. Van Eenoo, and M. Polet, “Searching for new long‐term urinary metabolites of metenolone and drostanolone using gas chromatography : mass spectrometry with a focus on non‐hydrolysed sulfates,” DRUG TESTING AND ANALYSIS, vol. 12, no. 8, pp. 1041–1053, 2020.
@article{8670188,
  abstract     = {{Sulfated metabolites have been shown to have potential as long-term markers of anabolic-androgenic steroid (AAS) abuse. In 2019, the compatibility of gas chromatography-mass spectrometry (GC-MS) with non-hydrolysed sulfated steroids was demonstrated, and this approach allowed the incorporation of these compounds in a broad GC-MS initial testing procedure at a later stage. However, research is needed to identify which are beneficial. In this study, a search for new long-term metabolites of two popular AAS, metenolone and drostanolone, was undertaken through two excretion studies each. The excretion samples were analysed using GC-chemical ionization-triple quadrupole MS (GC-CI-MS/MS) after the application of three separate sample preparation methodologies (i.e. hydrolysis withEscherichia coli-derived beta-glucuronidase,Helix pomatia-derived beta-glucuronidase/arylsulfatase and non-hydrolysed sulfated steroids). For metenolone, a non-hydrolysed sulfated metabolite, 1 beta-methyl-5 alpha-androstan-17-one-3 zeta-sulfate, was documented for the first time to provide the longest detection time of up to 17 days. This metabolite increased the detection time by nearly a factor of 2 in comparison with the currently monitored markers for metenolone in a routine doping control initial testing procedure. In the second excretion study, it prolonged the detection window by 25%. In the case of drostanolone, the non-hydrolysed sulfated metabolite with the longest detection time was the sulfated analogue of the main drostanolone metabolite (3 alpha-hydroxy-2 alpha-methyl-5 alpha-androstan-17-one) with a detection time of up to 24 days. However, the currently monitored main drostanolone metabolite in routine doping control, after hydrolysis of the glucuronide withE.coli, remained superior in detection time (i.e. up to 29 days).}},
  author       = {{Albertsdóttir, Aðalheiður Dóra and Van Gansbeke, Wim and Coppieters, Gilles and Balgimbekova, Kyzylkul and Van Eenoo, Peter and Polet, Michaël}},
  issn         = {{1942-7603}},
  journal      = {{DRUG TESTING AND ANALYSIS}},
  keywords     = {{Analytical Chemistry,Spectroscopy,Pharmaceutical Science,Environmental Chemistry,drostanolone,GC-MS,metenolone,steroids,sulfates,METHENOLONE ACETATE PRIMOBOLAN,ANABOLIC-STEROIDS,DOPING CONTROL,IDENTIFICATION,MESTEROLONE,SENSITIVITY,IONIZATION,VALIDATION,MISUSE}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1041--1053}},
  title        = {{Searching for new long‐term urinary metabolites of metenolone and drostanolone using gas chromatography : mass spectrometry with a focus on non‐hydrolysed sulfates}},
  url          = {{http://dx.doi.org/10.1002/dta.2818}},
  volume       = {{12}},
  year         = {{2020}},
}

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