Advanced search
1 file | 794.08 KB Add to list

Immune response in nonspecific mastitis : what can it tell us?

(2020) JOURNAL OF DAIRY SCIENCE. 103(6). p.5376-5386
Author
Organization
Abstract
We analyzed a large number of immune response parameters from quarter milk samples with distinct bacteriological and quarter somatic cell count (qSCC) statuses. Furthermore, we sought to explore and identify displayed immune response patterns in milk samples from mammary glands with nonspecific mastitis. Thus, 92 quarter milk samples from 28 cows were stratified into 4 groups, as follows: (1) 49 culture-negative control quarters with a low qSCC (<1 x 10(5) cells/mL) from 19 dairy cows (so-called healthy quarters); (2) 15 culture-negative quarters with high qSCC (>2 x 10(5) cells/mL; so-called quarters with nonspecific mastitis) from 10 dairy cows; (3) 8 culture-positive quarters with low qSCC (noninflammatory quarters with low qSCC) from 5 dairy cows; and (4) 20 culture-positive quarters with high qSCC (so-called truly infected quarters) from 8 dairy cows. Using flow cytometry, we evaluated the percentage of milk neutrophils and their viability, intracellular reactive oxygen species production, phagocytosis, and the expression of CD62L, CD11b, and CD44 for each of the 4 quarter strata. Furthermore, the percentage of monocyte/macrophages, B cells, and T lymphocyte subsets were evaluated by flow cytometry. Milk samples from bacteriologically negative quarters (both with a low and elevated qSCC) had a lower qSCC than those with bacteriologically positive outcomes (both with a low and elevated qSCC). As expected, the healthy quarters showed the lowest percentage of neutrophils and also showed a higher percentage of milk monocytes/macrophages and lower percentage of T lymphocytes than truly infected quarters. The most prominent result of the present study is that quarters with nonspecific mastitis showed the highest percentage of milk CD4(+) T lymphocytes. The healthy quarters had a lower percentage of apoptotic neutrophils than noninflammatory and truly infected quarters, although it did not differ from those from the quarters with nonspecific mastitis. Our study supports the role of differential cell counting in the diagnosis of mastitis, as the milk leukocyte populations markedly fluctuate under healthy and inflammatory conditions. Furthermore, an increase in milk CD4(+) T cells was associated with nonspecific mastitis, suggesting an increase in this leukocyte subpopulation is correlated with low bacterial shedding. Our study allows us to go further in our understanding of mammary gland immunity, providing further insights on potential protective mammary gland immunity, which we hypothesize can open new avenues for the development of novel targets that can promote bovine udder health.
Keywords
BOVINE MAMMARY-GLAND, SOMATIC-CELL COUNT, FLOW-CYTOMETRY, LONGITUDINAL EVALUATION, INFLAMMATORY REACTIONS, NEUTROPHIL APOPTOSIS, UDDER HEALTH, MILK SAMPLES, T-CELLS, LEUKOCYTE, mastitis, intramammary infection, differential leukocyte count, somatic cell count, dairy cow

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 794.08 KB

Citation

Please use this url to cite or link to this publication:

MLA
Nogueira De Souza, Fernando, et al. “Immune Response in Nonspecific Mastitis : What Can It Tell Us?” JOURNAL OF DAIRY SCIENCE, vol. 103, no. 6, 2020, pp. 5376–86, doi:10.3168/jds.2019-17022.
APA
Nogueira De Souza, F., Blagitz, M. G., Batista, C. F., Takano, P., V., Gargano, R. G., Diniz, S. A., … Della Libera, A. M. M. P. (2020). Immune response in nonspecific mastitis : what can it tell us? JOURNAL OF DAIRY SCIENCE, 103(6), 5376–5386. https://doi.org/10.3168/jds.2019-17022
Chicago author-date
Nogueira De Souza, Fernando, M. G. Blagitz, C. F. Batista, V Takano, P., R. G. Gargano, S. A. Diniz, M. X. Silva, et al. 2020. “Immune Response in Nonspecific Mastitis : What Can It Tell Us?” JOURNAL OF DAIRY SCIENCE 103 (6): 5376–86. https://doi.org/10.3168/jds.2019-17022.
Chicago author-date (all authors)
Nogueira De Souza, Fernando, M. G. Blagitz, C. F. Batista, V Takano, P., R. G. Gargano, S. A. Diniz, M. X. Silva, J. A. Ferronatto, K. R. Santos, M. B. Heinemann, Sarne De Vliegher, and A. M. M. P. Della Libera. 2020. “Immune Response in Nonspecific Mastitis : What Can It Tell Us?” JOURNAL OF DAIRY SCIENCE 103 (6): 5376–5386. doi:10.3168/jds.2019-17022.
Vancouver
1.
Nogueira De Souza F, Blagitz MG, Batista CF, Takano, P. V, Gargano RG, Diniz SA, et al. Immune response in nonspecific mastitis : what can it tell us? JOURNAL OF DAIRY SCIENCE. 2020;103(6):5376–86.
IEEE
[1]
F. Nogueira De Souza et al., “Immune response in nonspecific mastitis : what can it tell us?,” JOURNAL OF DAIRY SCIENCE, vol. 103, no. 6, pp. 5376–5386, 2020.
@article{8669333,
  abstract     = {We analyzed a large number of immune response parameters from quarter milk samples with distinct bacteriological and quarter somatic cell count (qSCC) statuses. Furthermore, we sought to explore and identify displayed immune response patterns in milk samples from mammary glands with nonspecific mastitis. Thus, 92 quarter milk samples from 28 cows were stratified into 4 groups, as follows: (1) 49 culture-negative control quarters with a low qSCC (<1 x 10(5) cells/mL) from 19 dairy cows (so-called healthy quarters); (2) 15 culture-negative quarters with high qSCC (>2 x 10(5) cells/mL; so-called quarters with nonspecific mastitis) from 10 dairy cows; (3) 8 culture-positive quarters with low qSCC (noninflammatory quarters with low qSCC) from 5 dairy cows; and (4) 20 culture-positive quarters with high qSCC (so-called truly infected quarters) from 8 dairy cows. Using flow cytometry, we evaluated the percentage of milk neutrophils and their viability, intracellular reactive oxygen species production, phagocytosis, and the expression of CD62L, CD11b, and CD44 for each of the 4 quarter strata. Furthermore, the percentage of monocyte/macrophages, B cells, and T lymphocyte subsets were evaluated by flow cytometry. Milk samples from bacteriologically negative quarters (both with a low and elevated qSCC) had a lower qSCC than those with bacteriologically positive outcomes (both with a low and elevated qSCC). As expected, the healthy quarters showed the lowest percentage of neutrophils and also showed a higher percentage of milk monocytes/macrophages and lower percentage of T lymphocytes than truly infected quarters. The most prominent result of the present study is that quarters with nonspecific mastitis showed the highest percentage of milk CD4(+) T lymphocytes. The healthy quarters had a lower percentage of apoptotic neutrophils than noninflammatory and truly infected quarters, although it did not differ from those from the quarters with nonspecific mastitis. Our study supports the role of differential cell counting in the diagnosis of mastitis, as the milk leukocyte populations markedly fluctuate under healthy and inflammatory conditions. Furthermore, an increase in milk CD4(+) T cells was associated with nonspecific mastitis, suggesting an increase in this leukocyte subpopulation is correlated with low bacterial shedding. Our study allows us to go further in our understanding of mammary gland immunity, providing further insights on potential protective mammary gland immunity, which we hypothesize can open new avenues for the development of novel targets that can promote bovine udder health.},
  author       = {Nogueira De Souza, Fernando and Blagitz, M. G. and Batista, C. F. and Takano, P., V and Gargano, R. G. and Diniz, S. A. and Silva, M. X. and Ferronatto, J. A. and Santos, K. R. and Heinemann, M. B. and De Vliegher, Sarne and Della Libera, A. M. M. P.},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {BOVINE MAMMARY-GLAND,SOMATIC-CELL COUNT,FLOW-CYTOMETRY,LONGITUDINAL EVALUATION,INFLAMMATORY REACTIONS,NEUTROPHIL APOPTOSIS,UDDER HEALTH,MILK SAMPLES,T-CELLS,LEUKOCYTE,mastitis,intramammary infection,differential leukocyte count,somatic cell count,dairy cow},
  language     = {eng},
  number       = {6},
  pages        = {5376--5386},
  title        = {Immune response in nonspecific mastitis : what can it tell us?},
  url          = {http://dx.doi.org/10.3168/jds.2019-17022},
  volume       = {103},
  year         = {2020},
}

Altmetric
View in Altmetric
Web of Science
Times cited: