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Rocks in the auxin stream : wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration

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Abstract
Plants are known for their outstanding capacity to recover from various wounds and injuries. However, it remains largely unknown how plants sense diverse forms of injury and canalize existing developmental processes into the execution of a correct regenerative response. Auxin, a cardinal plant hormone with morphogen-like properties, has been previously implicated in the recovery from diverse types of wounding and organ loss. Here, through a combination of cellular imaging and in silico modeling, we demonstrate that vascular stem cell death obstructs the polar auxin flux, much alike rocks in a stream, and causes it to accumulate in the endodermis. This in turn grants the endodermal cells the capacity to undergo periclinal cell division to repopulate the vascular stem cell pool. Replenishment of the vasculature by the endodermis depends on the transcription factor ERF115, a wound-inducible regulator of stem cell division. Although not the primary inducer, auxin is required to maintain ERF115 expression. Conversely, ERF115 sensitizes cells to auxin by activating ARF5/MONOPTEROS, an auxin-responsive transcription factor involved in the global auxin response, tissue patterning, and organ formation. Together, the wound-induced auxin accumulation and ERF115 expression grant the endodermal cells stem cell activity. Our work provides a mechanistic model for wound-induced stem cell regeneration in which ERF115 acts as a wound-inducible stem cell organizer that interprets wound-induced auxin maxima.
Keywords
Multidisciplinary, regeneration, auxin, ERF115, stem cells, Arabidopsis

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MLA
Canher, Balkan, et al. “Rocks in the Auxin Stream : Wound-Induced Auxin Accumulation and ERF115 Expression Synergistically Drive Stem Cell Regeneration.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 117, no. 28, 2020, pp. 16667–77, doi:10.1073/pnas.2006620117.
APA
Canher, B., Heyman, J., Savina, M., Devendran, A., Eekhout, T., Vercauteren, I., … De Veylder, L. (2020). Rocks in the auxin stream : wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 117(28), 16667–16677. https://doi.org/10.1073/pnas.2006620117
Chicago author-date
Canher, Balkan, Jefri Heyman, Maria Savina, Ajay Devendran, Thomas Eekhout, Ilse Vercauteren, Els Prinsen, et al. 2020. “Rocks in the Auxin Stream : Wound-Induced Auxin Accumulation and ERF115 Expression Synergistically Drive Stem Cell Regeneration.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117 (28): 16667–77. https://doi.org/10.1073/pnas.2006620117.
Chicago author-date (all authors)
Canher, Balkan, Jefri Heyman, Maria Savina, Ajay Devendran, Thomas Eekhout, Ilse Vercauteren, Els Prinsen, Rotem Matosevich, Jian Xu, Victoria Mironova, and Lieven De Veylder. 2020. “Rocks in the Auxin Stream : Wound-Induced Auxin Accumulation and ERF115 Expression Synergistically Drive Stem Cell Regeneration.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117 (28): 16667–16677. doi:10.1073/pnas.2006620117.
Vancouver
1.
Canher B, Heyman J, Savina M, Devendran A, Eekhout T, Vercauteren I, et al. Rocks in the auxin stream : wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2020;117(28):16667–77.
IEEE
[1]
B. Canher et al., “Rocks in the auxin stream : wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 117, no. 28, pp. 16667–16677, 2020.
@article{8668951,
  abstract     = {Plants are known for their outstanding capacity to recover from various wounds and injuries. However, it remains largely unknown how plants sense diverse forms of injury and canalize existing developmental processes into the execution of a correct regenerative response. Auxin, a cardinal plant hormone with morphogen-like properties, has been previously implicated in the recovery from diverse types of wounding and organ loss. Here, through a combination of cellular imaging and in silico modeling, we demonstrate that vascular stem cell death obstructs the polar auxin flux, much alike rocks in a stream, and causes it to accumulate in the endodermis. This in turn grants the endodermal cells the capacity to undergo periclinal cell division to repopulate the vascular stem cell pool. Replenishment of the vasculature by the endodermis depends on the transcription factor ERF115, a wound-inducible regulator of stem cell division. Although not the primary inducer, auxin is required to maintain ERF115 expression. Conversely, ERF115 sensitizes cells to auxin by activating ARF5/MONOPTEROS, an auxin-responsive transcription factor involved in the global auxin response, tissue patterning, and organ formation. Together, the wound-induced auxin accumulation and ERF115 expression grant the endodermal cells stem cell activity. Our work provides a mechanistic model for wound-induced stem cell regeneration in which ERF115 acts as a wound-inducible stem cell organizer that interprets wound-induced auxin maxima.},
  author       = {Canher, Balkan and Heyman, Jefri and Savina, Maria and Devendran, Ajay and Eekhout, Thomas and Vercauteren, Ilse and Prinsen, Els and Matosevich, Rotem and Xu, Jian and Mironova, Victoria and De Veylder, Lieven},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keywords     = {Multidisciplinary,regeneration,auxin,ERF115,stem cells,Arabidopsis},
  language     = {eng},
  number       = {28},
  pages        = {16667--16677},
  title        = {Rocks in the auxin stream : wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration},
  url          = {http://dx.doi.org/10.1073/pnas.2006620117},
  volume       = {117},
  year         = {2020},
}

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