@article{8668025,
  abstract     = {{Biomaterials engineered with specific cell binding sites, tunable mechanical properties, and complex architectures are essential to control cell adhesion and proliferation. The influence of the local properties, such as the local hardness and stability on the interaction with cells, has not been yet fully understood and exploited. This is particularly relevant for hydrogels, very promising materials with, unfortunately, poor cell adhesion properties, attributed mostly to their softness. Here, we propose a new approach for producing hybrid hydrogels by functionalizing them with particles and performing a thermal treatment. Exploring the interaction of cells with these materials we introduce a new concept, cells-grabbing-onto-particles, a facilitation of the cell adhesion through modulation of local properties. The approach is implemented on alginate hydrogels typically unsuitable for cell growth by turning them into a very effective cell culture growth platform. Specifically, alginate hydrogels are bio-mineralized with calcium carbonate (CaCO3) particles, where an additional thermal annealing (T-A) process has been applied. The local Young's modulus of new T-A treated hybrid hydrogels has increased to over 3 MPa on areas of hydrogels containing particles and to around 1 MPa on areas without particles, which is drastically different from 130 to 180 kPa values for unmodified hydrogels. Intriguingly, our results show that enhancement of local mechanical properties alone is a necessary, but insufficient, condition; the particles must be stably fixed in gels for cell growth and proliferation. Extended for hydrogels functionalized with silica particles too, the cells-grabon-particles concept is shown applicable to different materials and cells for cell biology and tissue engineering.}},
  author       = {{Abalymov, Anatolii and Van der Meeren, Louis and Saveleva, Mariia and Prikhozhdenko, Ekaterina and Dewettinck, Koen and Parakhonskiy, Bogdan and Skirtach, Andre}},
  issn         = {{2373-9878}},
  journal      = {{ACS BIOMATERIALS SCIENCE & ENGINEERING}},
  keywords     = {{hydrogels,biomineralization,particles,focal adhesion,CaCO3,tempetature,EXTRACELLULAR-MATRIX,MINERALIZATION,COMPOSITES,MEMBRANES,DELIVERY,SPECTRA,GROWTH}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{3933--3944}},
  title        = {{Cells-grab-on particles : a novel approach to control cell focal adhesion on hybrid thermally annealed hydrogels}},
  url          = {{http://doi.org/10.1021/acsbiomaterials.0c00119}},
  volume       = {{6}},
  year         = {{2020}},
}

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