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Temperature window for encapsulation of an enzyme into thermally shrunk, CaCO3 templated polyelectrolyte multilayer capsules

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Abstract
Encapsulation of enzymes allows to preserve their biological activities in various environmental conditions, such as exposure to elevated temperature or to proteases. This is particularly relevant for in vivo applications, where proteases represent a severe obstacle to maintaining the activity of enzymes. Polyelectrolyte multilayer capsules are suitable for enzyme encapsulation, where CaCO3 particles and temperature‐dependent capsule formation are the best templates and the most adequate method, respectively. In this work, these two areas are combined and, ALP (alkaline phosphatase), which is a robust and therapeutically relevant enzyme, is encapsulated into thermally shrunk polyelectrolyte multilayer (PDADMAC/PSS)4 capsules templated on calcium carbonate particles (original average diameter: ≈3.5 µm). The activity of the encapsulated enzyme and the optimal temperature range for encapsulation are investigated. The enzymatic activity is almost four times higher upon encapsulation when the temperature range for encapsulation is situated just above the glass transition temperature (40 °C), while its optimal conditions are dictated, on the one hand, by the enzyme activity (better at lower temperatures) and, on the other hand, by the size and mechanical properties of capsules (better at higher temperatures).
Keywords
Calcium carbonate, enzymes, layer-by-layer, LbL, temperature, thermal, BY-LAYER ASSEMBLIES, GLASS-TRANSITION, DRUG-DELIVERY, LBL CAPSULES, MICROCAPSULES, RELEASE, PH, DEGRADATION, FABRICATION, BEHAVIOR

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MLA
Van der Meeren, Louis, et al. “Temperature Window for Encapsulation of an Enzyme into Thermally Shrunk, CaCO3 Templated Polyelectrolyte Multilayer Capsules.” MACROMOLECULAR BIOSCIENCE, vol. 20, no. 7, 2020, doi:10.1002/mabi.202000081.
APA
Van der Meeren, L., Li, J., Konrad, M., Skirtach, A., Volodkin, D., & Parakhonskiy, B. (2020). Temperature window for encapsulation of an enzyme into thermally shrunk, CaCO3 templated polyelectrolyte multilayer capsules. MACROMOLECULAR BIOSCIENCE, 20(7). https://doi.org/10.1002/mabi.202000081
Chicago author-date
Van der Meeren, Louis, Jie Li, Manfred Konrad, Andre Skirtach, Dmitry Volodkin, and Bogdan Parakhonskiy. 2020. “Temperature Window for Encapsulation of an Enzyme into Thermally Shrunk, CaCO3 Templated Polyelectrolyte Multilayer Capsules.” MACROMOLECULAR BIOSCIENCE 20 (7). https://doi.org/10.1002/mabi.202000081.
Chicago author-date (all authors)
Van der Meeren, Louis, Jie Li, Manfred Konrad, Andre Skirtach, Dmitry Volodkin, and Bogdan Parakhonskiy. 2020. “Temperature Window for Encapsulation of an Enzyme into Thermally Shrunk, CaCO3 Templated Polyelectrolyte Multilayer Capsules.” MACROMOLECULAR BIOSCIENCE 20 (7). doi:10.1002/mabi.202000081.
Vancouver
1.
Van der Meeren L, Li J, Konrad M, Skirtach A, Volodkin D, Parakhonskiy B. Temperature window for encapsulation of an enzyme into thermally shrunk, CaCO3 templated polyelectrolyte multilayer capsules. MACROMOLECULAR BIOSCIENCE. 2020;20(7).
IEEE
[1]
L. Van der Meeren, J. Li, M. Konrad, A. Skirtach, D. Volodkin, and B. Parakhonskiy, “Temperature window for encapsulation of an enzyme into thermally shrunk, CaCO3 templated polyelectrolyte multilayer capsules,” MACROMOLECULAR BIOSCIENCE, vol. 20, no. 7, 2020.
@article{8667992,
  abstract     = {{Encapsulation of enzymes allows to preserve their biological activities in various environmental conditions, such as exposure to elevated temperature or to proteases. This is particularly relevant for in vivo applications, where proteases represent a severe obstacle to maintaining the activity of enzymes. Polyelectrolyte multilayer capsules are suitable for enzyme encapsulation, where CaCO3 particles and temperature‐dependent capsule formation are the best templates and the most adequate method, respectively. In this work, these two areas are combined and, ALP (alkaline phosphatase), which is a robust and therapeutically relevant enzyme, is encapsulated into thermally shrunk polyelectrolyte multilayer (PDADMAC/PSS)4 capsules templated on calcium carbonate particles (original average diameter: ≈3.5 µm). The activity of the encapsulated enzyme and the optimal temperature range for encapsulation are investigated. The enzymatic activity is almost four times higher upon encapsulation when the temperature range for encapsulation is situated just above the glass transition temperature (40 °C), while its optimal conditions are dictated, on the one hand, by the enzyme activity (better at lower temperatures) and, on the other hand, by the size and mechanical properties of capsules (better at higher temperatures).}},
  articleno    = {{2000081}},
  author       = {{Van der Meeren, Louis and Li, Jie and Konrad, Manfred and Skirtach, Andre and Volodkin, Dmitry and Parakhonskiy, Bogdan}},
  issn         = {{1616-5187}},
  journal      = {{MACROMOLECULAR BIOSCIENCE}},
  keywords     = {{Calcium carbonate,enzymes,layer-by-layer,LbL,temperature,thermal,BY-LAYER ASSEMBLIES,GLASS-TRANSITION,DRUG-DELIVERY,LBL CAPSULES,MICROCAPSULES,RELEASE,PH,DEGRADATION,FABRICATION,BEHAVIOR}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{7}},
  title        = {{Temperature window for encapsulation of an enzyme into thermally shrunk, CaCO3 templated polyelectrolyte multilayer capsules}},
  url          = {{http://dx.doi.org/10.1002/mabi.202000081}},
  volume       = {{20}},
  year         = {{2020}},
}

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