
Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials
- Author
- Anatolii Abalymov, Louis Van der Meeren (UGent) , Andre Skirtach (UGent) and Bogdan Parakhonskiy (UGent)
- Organization
- Project
- Abstract
- Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology. The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques. For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering. Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties. In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonates submicron particles. Determination of key parameters, which influence a successful hydroxyapatite generation, were done via the principal component analysis of 18 parameters (Young’s modulus of the hydrogel and particles, particles size and mass) and cell behaviour at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production and cells area) obtained by characterizing such composite hydrogel. It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young’s modulus in a hydrogel – a desirable property for cell adhesion. The presented here detailed analysis allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
- Keywords
- hydrogels, mineralization, particles, ossification, principal component analysis, BONE TISSUE, GELLAN GUM, MAGNESIUM CARBONATE, BIOMATERIALS, CALCIUM, REPAIR, MINERALIZATION, REGENERATION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8667991
- MLA
- Abalymov, Anatolii, et al. “Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 35, 2020, pp. 38862–72, doi:10.1021/acsami.0c06641.
- APA
- Abalymov, A., Van der Meeren, L., Skirtach, A., & Parakhonskiy, B. (2020). Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials. ACS APPLIED MATERIALS & INTERFACES, 12(35), 38862–38872. https://doi.org/10.1021/acsami.0c06641
- Chicago author-date
- Abalymov, Anatolii, Louis Van der Meeren, Andre Skirtach, and Bogdan Parakhonskiy. 2020. “Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.” ACS APPLIED MATERIALS & INTERFACES 12 (35): 38862–72. https://doi.org/10.1021/acsami.0c06641.
- Chicago author-date (all authors)
- Abalymov, Anatolii, Louis Van der Meeren, Andre Skirtach, and Bogdan Parakhonskiy. 2020. “Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.” ACS APPLIED MATERIALS & INTERFACES 12 (35): 38862–38872. doi:10.1021/acsami.0c06641.
- Vancouver
- 1.Abalymov A, Van der Meeren L, Skirtach A, Parakhonskiy B. Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials. ACS APPLIED MATERIALS & INTERFACES. 2020;12(35):38862–72.
- IEEE
- [1]A. Abalymov, L. Van der Meeren, A. Skirtach, and B. Parakhonskiy, “Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials,” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 35, pp. 38862–38872, 2020.
@article{8667991, abstract = {{Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology. The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques. For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering. Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties. In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonates submicron particles. Determination of key parameters, which influence a successful hydroxyapatite generation, were done via the principal component analysis of 18 parameters (Young’s modulus of the hydrogel and particles, particles size and mass) and cell behaviour at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production and cells area) obtained by characterizing such composite hydrogel. It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young’s modulus in a hydrogel – a desirable property for cell adhesion. The presented here detailed analysis allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.}}, author = {{Abalymov, Anatolii and Van der Meeren, Louis and Skirtach, Andre and Parakhonskiy, Bogdan}}, issn = {{1944-8244}}, journal = {{ACS APPLIED MATERIALS & INTERFACES}}, keywords = {{hydrogels,mineralization,particles,ossification,principal component analysis,BONE TISSUE,GELLAN GUM,MAGNESIUM CARBONATE,BIOMATERIALS,CALCIUM,REPAIR,MINERALIZATION,REGENERATION}}, language = {{eng}}, number = {{35}}, pages = {{38862--38872}}, title = {{Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials}}, url = {{http://dx.doi.org/10.1021/acsami.0c06641}}, volume = {{12}}, year = {{2020}}, }
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