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Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials

Anatolii Abalymov (UGent) , Louis Van der Meeren (UGent) , Andre Skirtach (UGent) and Bogdan Parakhonskiy (UGent)
(2020) ACS APPLIED MATERIALS & INTERFACES. 12(35). p.38862-38872
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Abstract
Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology. The major challenge is the labor-intensive data mining as well as requirements of the number of advanced techniques. For example, hydrogel-based biomaterials with cell-binding sites, tunable mechanical properties and complex architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering. Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties. In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them with Ca/Mg carbonates submicron particles. Determination of key parameters, which influence a successful hydroxyapatite generation, were done via the principal component analysis of 18 parameters (Young’s modulus of the hydrogel and particles, particles size and mass) and cell behaviour at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production and cells area) obtained by characterizing such composite hydrogel. It is determined that the particles size and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young’s modulus in a hydrogel – a desirable property for cell adhesion. The presented here detailed analysis allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.
Keywords
hydrogels, mineralization, particles, ossification, principal component analysis, BONE TISSUE, GELLAN GUM, MAGNESIUM CARBONATE, BIOMATERIALS, CALCIUM, REPAIR, MINERALIZATION, REGENERATION

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Citation

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MLA
Abalymov, Anatolii, et al. “Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 35, 2020, pp. 38862–72, doi:10.1021/acsami.0c06641.
APA
Abalymov, A., Van der Meeren, L., Skirtach, A., & Parakhonskiy, B. (2020). Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials. ACS APPLIED MATERIALS & INTERFACES, 12(35), 38862–38872. https://doi.org/10.1021/acsami.0c06641
Chicago author-date
Abalymov, Anatolii, Louis Van der Meeren, Andre Skirtach, and Bogdan Parakhonskiy. 2020. “Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.” ACS APPLIED MATERIALS & INTERFACES 12 (35): 38862–72. https://doi.org/10.1021/acsami.0c06641.
Chicago author-date (all authors)
Abalymov, Anatolii, Louis Van der Meeren, Andre Skirtach, and Bogdan Parakhonskiy. 2020. “Identification and Analysis of Key Parameters for the Ossification on Particle Functionalized Composites Hydrogel Materials.” ACS APPLIED MATERIALS & INTERFACES 12 (35): 38862–38872. doi:10.1021/acsami.0c06641.
Vancouver
1.
Abalymov A, Van der Meeren L, Skirtach A, Parakhonskiy B. Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials. ACS APPLIED MATERIALS & INTERFACES. 2020;12(35):38862–72.
IEEE
[1]
A. Abalymov, L. Van der Meeren, A. Skirtach, and B. Parakhonskiy, “Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials,” ACS APPLIED MATERIALS & INTERFACES, vol. 12, no. 35, pp. 38862–38872, 2020.
@article{8667991,
  abstract     = {{Developing materials for tissue engineering and studying the mechanisms of cell adhesion is a complex and multifactor process that needs analysis using physical chemistry and biology. The major challenge is the labor-intensive data mining as well as requirements  of  the  number  of  advanced  techniques.  For example,  hydrogel-based  biomaterials  with  cell-binding  sites,  tunable mechanical properties and complex  architectures have emerged as a powerful tool to control cell adhesion and proliferation for tissue engineering. Composite hydrogels could be used for bone tissue regeneration, but they exhibit poor ossification properties. In current work, we have designed new osteoinductive gellan gum hydrogels by a thermal annealing approach and consequently functionalized them  with  Ca/Mg  carbonates  submicron  particles.  Determination  of  key  parameters,  which  influence  a  successful  hydroxyapatite generation, were  done  via  the  principal  component  analysis  of  18  parameters  (Young’s  modulus  of  the  hydrogel  and  particles, particles size and mass) and cell behaviour at various time points (like viability, numbers of the cells, rate of alkaline phosphatase production  and  cells  area)  obtained  by  characterizing  such  composite  hydrogel.  It  is  determined  that  the  particles  size  and concentration of calcium ions have a dominant effect on the hydroxyapatite formation, because of providing local areas with a high Young’s  modulus  in  a  hydrogel  –  a  desirable  property  for  cell  adhesion.  The  presented  here  detailed  analysis  allows identifying hydrogels for cell growth applications, while on the other hand, material properties can be predicted, and their overall number can be minimized leading to efficient optimization of bone reconstruction and other cell growth applications.}},
  author       = {{Abalymov, Anatolii and Van der Meeren, Louis and Skirtach, Andre and Parakhonskiy, Bogdan}},
  issn         = {{1944-8244}},
  journal      = {{ACS APPLIED MATERIALS & INTERFACES}},
  keywords     = {{hydrogels,mineralization,particles,ossification,principal component analysis,BONE TISSUE,GELLAN GUM,MAGNESIUM CARBONATE,BIOMATERIALS,CALCIUM,REPAIR,MINERALIZATION,REGENERATION}},
  language     = {{eng}},
  number       = {{35}},
  pages        = {{38862--38872}},
  title        = {{Identification and analysis of key parameters for the ossification on particle functionalized composites hydrogel materials}},
  url          = {{http://dx.doi.org/10.1021/acsami.0c06641}},
  volume       = {{12}},
  year         = {{2020}},
}
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