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Acute symptomatic seizures following intracerebral hemorrhage in the rat collagenase model

Charlotte Germonpré, Silke Proesmans (UGent) , Charlotte Bouckaert, Latoya Stevens, Mathieu Sprengers (UGent) , Kristl Vonck (UGent) , Evelien Carrette (UGent) , Wytse Wadman (UGent) , Paul Boon (UGent) , Robrecht Raedt (UGent) , et al.
(2020) EPILEPSY RESEARCH. 164.
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Abstract
Background and purpose: Intracerebral hemorrhage (ICH) is a known risk factor for the development of seizures, but little is known about the pathophysiology of seizures in the acute phase post-ICH and their influence on functional outcome. With the use of an animal model, the underlying pathophysiology could be further unraveled. The aim of our study was to optimize the rat collagenase stroke model for the detection of acute symptomatic seizures using video-EEG monitoring. Methods: Male Sprague-Dawley rats were implanted with scalp electrodes and a craniotomy was made for later injection of collagenase. After one week of baseline video-EEG recording, rats were injected with 0.6 U collagenase in 0.7 mu L saline in left striatum, in close proximity of the piriform cortex, and immediately reconnected to the video-EEG setup for 7 days. Occurrence of clinical and electrographic seizures was assessed and functional deficits were evaluated on several time points using the cylinder test, Neurological Deficit Scale (NDS) and forelimb placing test. At day 7 post-ICH, animals were euthanized. The volume and cortical involvement of the hemorrhage were assessed by histological examination of the brain tissue, using Cresyl violet stain. Results: Collagenase injection induced ICH in all animals with a mean volume of 27 mm(3) (SEM 7 mm(3), range 4-92 mm(3)). Functional deficits were present in all animals injected with collagenase (pre-ICH vs post-ICH, p< 0.001). Epileptic seizures occurred in 5/11 animals and started between 1 and 61 h after ICH induction. Behavioral changes were observed in 13/15 seizures. Conclusions: Injecting rats with 0.6 U of collagenase is a useful model to study the occurrence of acute symptomatic seizures post-ICH as it results in ICH in all animals without mortality, 45% incidence of ICH-induced acute symptomatic seizures and measurable functional deficits.
Keywords
TERM VIDEO-EEG, BRAIN-INJURY, STROKE, PREDICTORS, VOLUME, DISCHARGES, INJECTION, OUTCOMES, Stroke, Intracerebral hemorrhage, Video-EEG monitoring, Acute, symptomatic seizures, Epilepsy

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MLA
Germonpré, Charlotte, et al. “Acute Symptomatic Seizures Following Intracerebral Hemorrhage in the Rat Collagenase Model.” EPILEPSY RESEARCH, vol. 164, 2020, doi:10.1016/j.eplepsyres.2020.106364.
APA
Germonpré, C., Proesmans, S., Bouckaert, C., Stevens, L., Sprengers, M., Vonck, K., … De Herdt, V. (2020). Acute symptomatic seizures following intracerebral hemorrhage in the rat collagenase model. EPILEPSY RESEARCH, 164. https://doi.org/10.1016/j.eplepsyres.2020.106364
Chicago author-date
Germonpré, Charlotte, Silke Proesmans, Charlotte Bouckaert, Latoya Stevens, Mathieu Sprengers, Kristl Vonck, Evelien Carrette, et al. 2020. “Acute Symptomatic Seizures Following Intracerebral Hemorrhage in the Rat Collagenase Model.” EPILEPSY RESEARCH 164. https://doi.org/10.1016/j.eplepsyres.2020.106364.
Chicago author-date (all authors)
Germonpré, Charlotte, Silke Proesmans, Charlotte Bouckaert, Latoya Stevens, Mathieu Sprengers, Kristl Vonck, Evelien Carrette, Wytse Wadman, Paul Boon, Robrecht Raedt, and Veerle De Herdt. 2020. “Acute Symptomatic Seizures Following Intracerebral Hemorrhage in the Rat Collagenase Model.” EPILEPSY RESEARCH 164. doi:10.1016/j.eplepsyres.2020.106364.
Vancouver
1.
Germonpré C, Proesmans S, Bouckaert C, Stevens L, Sprengers M, Vonck K, et al. Acute symptomatic seizures following intracerebral hemorrhage in the rat collagenase model. EPILEPSY RESEARCH. 2020;164.
IEEE
[1]
C. Germonpré et al., “Acute symptomatic seizures following intracerebral hemorrhage in the rat collagenase model,” EPILEPSY RESEARCH, vol. 164, 2020.
@article{8667537,
  abstract     = {{Background and purpose: Intracerebral hemorrhage (ICH) is a known risk factor for the development of seizures, but little is known about the pathophysiology of seizures in the acute phase post-ICH and their influence on functional outcome. With the use of an animal model, the underlying pathophysiology could be further unraveled. The aim of our study was to optimize the rat collagenase stroke model for the detection of acute symptomatic seizures using video-EEG monitoring. Methods: Male Sprague-Dawley rats were implanted with scalp electrodes and a craniotomy was made for later injection of collagenase. After one week of baseline video-EEG recording, rats were injected with 0.6 U collagenase in 0.7 mu L saline in left striatum, in close proximity of the piriform cortex, and immediately reconnected to the video-EEG setup for 7 days. Occurrence of clinical and electrographic seizures was assessed and functional deficits were evaluated on several time points using the cylinder test, Neurological Deficit Scale (NDS) and forelimb placing test. At day 7 post-ICH, animals were euthanized. The volume and cortical involvement of the hemorrhage were assessed by histological examination of the brain tissue, using Cresyl violet stain. Results: Collagenase injection induced ICH in all animals with a mean volume of 27 mm(3) (SEM 7 mm(3), range 4-92 mm(3)). Functional deficits were present in all animals injected with collagenase (pre-ICH vs post-ICH, p< 0.001). Epileptic seizures occurred in 5/11 animals and started between 1 and 61 h after ICH induction. Behavioral changes were observed in 13/15 seizures. Conclusions: Injecting rats with 0.6 U of collagenase is a useful model to study the occurrence of acute symptomatic seizures post-ICH as it results in ICH in all animals without mortality, 45% incidence of ICH-induced acute symptomatic seizures and measurable functional deficits.}},
  articleno    = {{106364}},
  author       = {{Germonpré, Charlotte and Proesmans, Silke and Bouckaert, Charlotte and Stevens, Latoya and Sprengers, Mathieu and Vonck, Kristl and Carrette, Evelien and Wadman, Wytse and Boon, Paul and Raedt, Robrecht and De Herdt, Veerle}},
  issn         = {{0920-1211}},
  journal      = {{EPILEPSY RESEARCH}},
  keywords     = {{TERM VIDEO-EEG,BRAIN-INJURY,STROKE,PREDICTORS,VOLUME,DISCHARGES,INJECTION,OUTCOMES,Stroke,Intracerebral hemorrhage,Video-EEG monitoring,Acute,symptomatic seizures,Epilepsy}},
  language     = {{eng}},
  pages        = {{8}},
  title        = {{Acute symptomatic seizures following intracerebral hemorrhage in the rat collagenase model}},
  url          = {{http://doi.org/10.1016/j.eplepsyres.2020.106364}},
  volume       = {{164}},
  year         = {{2020}},
}
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