Multiplexed antibody detection with an array of silicon-on-insulator microring resonators
- Author
- Katrien De Vos (UGent) , Jordi Gironès Molera (UGent) , Tom Claes (UGent) , Yannick De Koninck (UGent) , Stepan Popelka, Etienne Schacht (UGent) , Roel Baets (UGent) and Peter Bienstman (UGent)
- Organization
- Abstract
- Optical cavities are considered promising devices for biosensing to satisfy the high demands of the growing proteomics market. We present a prototype sensor for multiplexed label-free monitoring of biomolecular interactions based on an array of three-by-four silicon-on-insulator microring resonators fabricated by standard complementary metal-oxide semicondutor (CMOS) technology. Parallel readout was performed with an infrared camera. We have demonstrated the simultaneous detection of several model antibodies in a highly specific way. For that, we integrated the optical chip with low-temperature polydimethylsiloxane (PDMS) packaging, enabling excellent compatibility with the receptor molecules. We obtain a very selective biomolecular interaction by grafting a 2.5-nm poly(ethylene glycol) layer to the silicon. Using silicon on insulator as the material platform offers high sensitivity through miniaturization and low-cost mass fabrication through standard CMOS technology steps. The sensors in this interrogation method are able to detect 3.4 pg/mm(2) of a protein overlayer or a theoretical total mass of about 74 ag. For this novel lab on a chip, the fabrication, chemistry, readout, and packaging are developed to be scalable to detect hundreds of affinity interactions within several minutes.
- Keywords
- biosensors, Silicon nanophotonics, waveguides, INTERFEROMETER, LABEL-FREE DETECTION, REFRACTIVE-INDEX, SENSORS, MICROARRAYS, BIOSENSOR, label-free, resonators, microfluidics
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-866750
- MLA
- De Vos, Katrien, et al. “Multiplexed Antibody Detection with an Array of Silicon-on-Insulator Microring Resonators.” IEEE PHOTONICS JOURNAL, vol. 1, no. 4, 2009, pp. 225–35, doi:10.1109/JPHOT.2009.2035433.
- APA
- De Vos, K., Gironès Molera, J., Claes, T., De Koninck, Y., Popelka, S., Schacht, E., … Bienstman, P. (2009). Multiplexed antibody detection with an array of silicon-on-insulator microring resonators. IEEE PHOTONICS JOURNAL, 1(4), 225–235. https://doi.org/10.1109/JPHOT.2009.2035433
- Chicago author-date
- De Vos, Katrien, Jordi Gironès Molera, Tom Claes, Yannick De Koninck, Stepan Popelka, Etienne Schacht, Roel Baets, and Peter Bienstman. 2009. “Multiplexed Antibody Detection with an Array of Silicon-on-Insulator Microring Resonators.” IEEE PHOTONICS JOURNAL 1 (4): 225–35. https://doi.org/10.1109/JPHOT.2009.2035433.
- Chicago author-date (all authors)
- De Vos, Katrien, Jordi Gironès Molera, Tom Claes, Yannick De Koninck, Stepan Popelka, Etienne Schacht, Roel Baets, and Peter Bienstman. 2009. “Multiplexed Antibody Detection with an Array of Silicon-on-Insulator Microring Resonators.” IEEE PHOTONICS JOURNAL 1 (4): 225–235. doi:10.1109/JPHOT.2009.2035433.
- Vancouver
- 1.De Vos K, Gironès Molera J, Claes T, De Koninck Y, Popelka S, Schacht E, et al. Multiplexed antibody detection with an array of silicon-on-insulator microring resonators. IEEE PHOTONICS JOURNAL. 2009;1(4):225–35.
- IEEE
- [1]K. De Vos et al., “Multiplexed antibody detection with an array of silicon-on-insulator microring resonators,” IEEE PHOTONICS JOURNAL, vol. 1, no. 4, pp. 225–235, 2009.
@article{866750, abstract = {{Optical cavities are considered promising devices for biosensing to satisfy the high demands of the growing proteomics market. We present a prototype sensor for multiplexed label-free monitoring of biomolecular interactions based on an array of three-by-four silicon-on-insulator microring resonators fabricated by standard complementary metal-oxide semicondutor (CMOS) technology. Parallel readout was performed with an infrared camera. We have demonstrated the simultaneous detection of several model antibodies in a highly specific way. For that, we integrated the optical chip with low-temperature polydimethylsiloxane (PDMS) packaging, enabling excellent compatibility with the receptor molecules. We obtain a very selective biomolecular interaction by grafting a 2.5-nm poly(ethylene glycol) layer to the silicon. Using silicon on insulator as the material platform offers high sensitivity through miniaturization and low-cost mass fabrication through standard CMOS technology steps. The sensors in this interrogation method are able to detect 3.4 pg/mm(2) of a protein overlayer or a theoretical total mass of about 74 ag. For this novel lab on a chip, the fabrication, chemistry, readout, and packaging are developed to be scalable to detect hundreds of affinity interactions within several minutes.}}, author = {{De Vos, Katrien and Gironès Molera, Jordi and Claes, Tom and De Koninck, Yannick and Popelka, Stepan and Schacht, Etienne and Baets, Roel and Bienstman, Peter}}, issn = {{1943-0655}}, journal = {{IEEE PHOTONICS JOURNAL}}, keywords = {{biosensors,Silicon nanophotonics,waveguides,INTERFEROMETER,LABEL-FREE DETECTION,REFRACTIVE-INDEX,SENSORS,MICROARRAYS,BIOSENSOR,label-free,resonators,microfluidics}}, language = {{eng}}, number = {{4}}, pages = {{225--235}}, title = {{Multiplexed antibody detection with an array of silicon-on-insulator microring resonators}}, url = {{http://doi.org/10.1109/JPHOT.2009.2035433}}, volume = {{1}}, year = {{2009}}, }
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