Advanced search
1 file | 1.46 MB Add to list

An antennae-specific odorant-binding protein is involved in Bactrocera dorsalis olfaction

Author
Organization
Abstract
Insect antennae are important olfactory organs that house high concentrations of odorant-binding proteins (OBPs) in the sensillum lymph. Previous studies in other insects have shown that OBPs play important roles in transporting odorants and enhancing the sensitivity of the olfactory system. However, the functions of OBPs in the oriental fruit fly, Bactrocera dorsalis, especially those specifically expressed in antennae, have not been fully elucidated. In this study, cDNA libraries were constructed from both the male and female antennal transcriptome, and twenty OBPs were identified in total. The expression profiles of these OBPs were examined in the adult antenna, head, thorax, leg, and abdomen of both sexes. Seven of the identified OBP genes had significantly higher expression in both the male and female antennae than in other tissues, while the transcript levels of the remaining OBPs varied across different tissues. Regarding the function of antenna-specific OBPs, we targeted Bdorsobp2 as a representative for further RNA interference (RNAi) and identified via electrophysiology a decrease in detection of a potential species-specific a potent attractant, methyl eugenol. Moreover, subsequent behavioral assay data showed that the behavioral response of B. dorsalis toward this odorant decreased when Bdorobp2 was silenced with injection of double-stranded RNA (dsRNA). Combined, these results support our initial hypothesis that antennae-specific OBPs are of critical importance for insect odorant detection, sensitivity, and behavior.
Keywords
oriental fruit fly, odorant-binding proteins, olfaction, antennae, dsRNA, ORIENTAL FRUIT-FLY, BUG ADELPHOCORIS-LINEOLATUS, METHYL EUGENOL, IDENTIFICATION, DIPTERA, TASTE, TEPHRITIDAE, SUPPRESSION, ATTRACTION, EXPRESSION

Downloads

  • fevo-08-00063.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 1.46 MB

Citation

Please use this url to cite or link to this publication:

MLA
Liu, Zhao, et al. “An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera Dorsalis Olfaction.” FRONTIERS IN ECOLOGY AND EVOLUTION, vol. 8, 2020, doi:10.3389/fevo.2020.00063.
APA
Liu, Z., Liang, X.-F., Xu, L., Keesey, I. W., Lei, Z.-R., Smagghe, G., & Wang, J.-J. (2020). An antennae-specific odorant-binding protein is involved in Bactrocera dorsalis olfaction. FRONTIERS IN ECOLOGY AND EVOLUTION, 8. https://doi.org/10.3389/fevo.2020.00063
Chicago author-date
Liu, Zhao, Xiao-Fei Liang, Li Xu, Ian W. Keesey, Zhong-Ren Lei, Guy Smagghe, and Jin-Jun Wang. 2020. “An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera Dorsalis Olfaction.” FRONTIERS IN ECOLOGY AND EVOLUTION 8. https://doi.org/10.3389/fevo.2020.00063.
Chicago author-date (all authors)
Liu, Zhao, Xiao-Fei Liang, Li Xu, Ian W. Keesey, Zhong-Ren Lei, Guy Smagghe, and Jin-Jun Wang. 2020. “An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera Dorsalis Olfaction.” FRONTIERS IN ECOLOGY AND EVOLUTION 8. doi:10.3389/fevo.2020.00063.
Vancouver
1.
Liu Z, Liang X-F, Xu L, Keesey IW, Lei Z-R, Smagghe G, et al. An antennae-specific odorant-binding protein is involved in Bactrocera dorsalis olfaction. FRONTIERS IN ECOLOGY AND EVOLUTION. 2020;8.
IEEE
[1]
Z. Liu et al., “An antennae-specific odorant-binding protein is involved in Bactrocera dorsalis olfaction,” FRONTIERS IN ECOLOGY AND EVOLUTION, vol. 8, 2020.
@article{8667126,
  abstract     = {Insect antennae are important olfactory organs that house high concentrations of odorant-binding proteins (OBPs) in the sensillum lymph. Previous studies in other insects have shown that OBPs play important roles in transporting odorants and enhancing the sensitivity of the olfactory system. However, the functions of OBPs in the oriental fruit fly, Bactrocera dorsalis, especially those specifically expressed in antennae, have not been fully elucidated. In this study, cDNA libraries were constructed from both the male and female antennal transcriptome, and twenty OBPs were identified in total. The expression profiles of these OBPs were examined in the adult antenna, head, thorax, leg, and abdomen of both sexes. Seven of the identified OBP genes had significantly higher expression in both the male and female antennae than in other tissues, while the transcript levels of the remaining OBPs varied across different tissues. Regarding the function of antenna-specific OBPs, we targeted Bdorsobp2 as a representative for further RNA interference (RNAi) and identified via electrophysiology a decrease in detection of a potential species-specific a potent attractant, methyl eugenol. Moreover, subsequent behavioral assay data showed that the behavioral response of B. dorsalis toward this odorant decreased when Bdorobp2 was silenced with injection of double-stranded RNA (dsRNA). Combined, these results support our initial hypothesis that antennae-specific OBPs are of critical importance for insect odorant detection, sensitivity, and behavior.},
  articleno    = {63},
  author       = {Liu, Zhao and Liang, Xiao-Fei and Xu, Li and Keesey, Ian W. and Lei, Zhong-Ren and Smagghe, Guy and Wang, Jin-Jun},
  issn         = {2296-701X},
  journal      = {FRONTIERS IN ECOLOGY AND EVOLUTION},
  keywords     = {oriental fruit fly,odorant-binding proteins,olfaction,antennae,dsRNA,ORIENTAL FRUIT-FLY,BUG ADELPHOCORIS-LINEOLATUS,METHYL EUGENOL,IDENTIFICATION,DIPTERA,TASTE,TEPHRITIDAE,SUPPRESSION,ATTRACTION,EXPRESSION},
  language     = {eng},
  pages        = {10},
  title        = {An antennae-specific odorant-binding protein is involved in Bactrocera dorsalis olfaction},
  url          = {http://dx.doi.org/10.3389/fevo.2020.00063},
  volume       = {8},
  year         = {2020},
}

Altmetric
View in Altmetric
Web of Science
Times cited: