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Evaluation of 3D printed gelatin-based scaffolds with varying pore size for MSC-based adipose tissue engineering

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Abstract
Adipose tissue engineering aims to provide solutions to patients who require tissue reconstruction following mastectomies or other soft tissue trauma. Mesenchymal stromal cells (MSCs) robustly differentiate into the adipogenic lineage and are attractive candidates for adipose tissue engineering. This work investigates whether pore size modulates adipogenic differentiation of MSCs toward identifying optimal scaffold pore size and whether pore size modulates spatial infiltration of adipogenically differentiated cells. To assess this, extrusion-based 3D printing is used to fabricate photo-crosslinkable gelatin-based scaffolds with pore sizes in the range of 200-600 mu m. The adipogenic differentiation of MSCs seeded onto these scaffolds is evaluated and robust lipid droplet formation is observed across all scaffold groups as early as after day 6 of culture. Expression of adipogenic genes on scaffolds increases significantly over time, compared to TCP controls. Furthermore, it is found that the spatial distribution of cells is dependent on the scaffold pore size, with larger pores leading to a more uniform spatial distribution of adipogenically differentiated cells. Overall, these data provide first insights into the role of scaffold pore size on MSC-based adipogenic differentiation and contribute toward the rational design of biomaterials for adipose tissue engineering in 3D volumetric spaces.
Keywords
ELASTICITY, SOFT, adipogenic differentiation, extrusion-based 3D-printing, hydrogel, mesenchymal stromal cells, pore size

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MLA
Tytgat, Liesbeth, et al. “Evaluation of 3D Printed Gelatin-Based Scaffolds with Varying Pore Size for MSC-Based Adipose Tissue Engineering.” MACROMOLECULAR BIOSCIENCE, vol. 20, no. 4, 2020, doi:10.1002/mabi.201900364.
APA
Tytgat, L., Kollert, M. R., Van Damme, L., Thienpont, H., Ottevaere, H., Duda, G. N., … Qazi, T. H. (2020). Evaluation of 3D printed gelatin-based scaffolds with varying pore size for MSC-based adipose tissue engineering. MACROMOLECULAR BIOSCIENCE, 20(4). https://doi.org/10.1002/mabi.201900364
Chicago author-date
Tytgat, Liesbeth, Matthias R. Kollert, Lana Van Damme, Hugo Thienpont, Heidi Ottevaere, Georg N. Duda, Sven Geissler, Peter Dubruel, Sandra Van Vlierberghe, and Taimoor H. Qazi. 2020. “Evaluation of 3D Printed Gelatin-Based Scaffolds with Varying Pore Size for MSC-Based Adipose Tissue Engineering.” MACROMOLECULAR BIOSCIENCE 20 (4). https://doi.org/10.1002/mabi.201900364.
Chicago author-date (all authors)
Tytgat, Liesbeth, Matthias R. Kollert, Lana Van Damme, Hugo Thienpont, Heidi Ottevaere, Georg N. Duda, Sven Geissler, Peter Dubruel, Sandra Van Vlierberghe, and Taimoor H. Qazi. 2020. “Evaluation of 3D Printed Gelatin-Based Scaffolds with Varying Pore Size for MSC-Based Adipose Tissue Engineering.” MACROMOLECULAR BIOSCIENCE 20 (4). doi:10.1002/mabi.201900364.
Vancouver
1.
Tytgat L, Kollert MR, Van Damme L, Thienpont H, Ottevaere H, Duda GN, et al. Evaluation of 3D printed gelatin-based scaffolds with varying pore size for MSC-based adipose tissue engineering. MACROMOLECULAR BIOSCIENCE. 2020;20(4).
IEEE
[1]
L. Tytgat et al., “Evaluation of 3D printed gelatin-based scaffolds with varying pore size for MSC-based adipose tissue engineering,” MACROMOLECULAR BIOSCIENCE, vol. 20, no. 4, 2020.
@article{8666900,
  abstract     = {Adipose tissue engineering aims to provide solutions to patients who require tissue reconstruction following mastectomies or other soft tissue trauma. Mesenchymal stromal cells (MSCs) robustly differentiate into the adipogenic lineage and are attractive candidates for adipose tissue engineering. This work investigates whether pore size modulates adipogenic differentiation of MSCs toward identifying optimal scaffold pore size and whether pore size modulates spatial infiltration of adipogenically differentiated cells. To assess this, extrusion-based 3D printing is used to fabricate photo-crosslinkable gelatin-based scaffolds with pore sizes in the range of 200-600 mu m. The adipogenic differentiation of MSCs seeded onto these scaffolds is evaluated and robust lipid droplet formation is observed across all scaffold groups as early as after day 6 of culture. Expression of adipogenic genes on scaffolds increases significantly over time, compared to TCP controls. Furthermore, it is found that the spatial distribution of cells is dependent on the scaffold pore size, with larger pores leading to a more uniform spatial distribution of adipogenically differentiated cells. Overall, these data provide first insights into the role of scaffold pore size on MSC-based adipogenic differentiation and contribute toward the rational design of biomaterials for adipose tissue engineering in 3D volumetric spaces.},
  articleno    = {1900364},
  author       = {Tytgat, Liesbeth and Kollert, Matthias R. and Van Damme, Lana and Thienpont, Hugo and Ottevaere, Heidi and Duda, Georg N. and Geissler, Sven and Dubruel, Peter and Van Vlierberghe, Sandra and Qazi, Taimoor H.},
  issn         = {1616-5187},
  journal      = {MACROMOLECULAR BIOSCIENCE},
  keywords     = {ELASTICITY,SOFT,adipogenic differentiation,extrusion-based 3D-printing,hydrogel,mesenchymal stromal cells,pore size},
  language     = {eng},
  number       = {4},
  pages        = {6},
  title        = {Evaluation of 3D printed gelatin-based scaffolds with varying pore size for MSC-based adipose tissue engineering},
  url          = {http://dx.doi.org/10.1002/mabi.201900364},
  volume       = {20},
  year         = {2020},
}

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