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Bioprinting predifferentiated adipose-derived mesenchymal stem cell spheroids with methacrylated gelatin ink for adipose tissue engineering

Julien Colle, Phillip Blondeel (UGent) , Axelle De Bruyne, Silke Bochar, Liesbeth Tytgat, Chris Vercruysse (UGent) , Sandra Van Vlierberghe (UGent) , Peter Dubruel (UGent) and Heidi Declercq (UGent)
(2020) JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE. 31(4).
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Abstract
The increasing number of mastectomies results in a greater demand for breast reconstruction characterized by simplicity and a low complication profile. Reconstructive surgeons are investigating tissue engineering (TE) strategies to overcome the current surgical drawbacks. 3D bioprinting is the rising technique for the fabrication of large tissue constructs which provides a potential solution for unmet clinical needs in breast reconstruction building on decades of experience in autologous fat grafting, adipose-derived mesenchymal stem cell (ASC) biology and TE. A scaffold was bioprinted using encapsulated ASC spheroids in methacrylated gelatin ink (GelMA). Uniform ASC spheroids with an ideal geometry and diameter for bioprinting were formed, using a high-throughput non-adhesive agarose microwell system. ASC spheroids in adipogenic differentiation medium (ADM) were evaluated through live/dead staining, histology (HE, Oil Red O), TEM and RT-qPCR. Viable spheroids were obtained for up to 14 days post-printing and showed multilocular microvacuoles and successful differentiation toward mature adipocytes shown by gene expression analysis. Moreover, spheroids were able to assemble at random in GelMA, creating a macrotissue. Combining the advantage of microtissues to self-assemble and the controlled organization by bioprinting technologies, these ASC spheroids can be useful as building blocks for the engineering of soft tissue implants.
Keywords
DIFFERENTIATION, HYDROGELS, CONSTRUCTS, COMPONENTS, DESIGN

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MLA
Colle, Julien, et al. “Bioprinting Predifferentiated Adipose-Derived Mesenchymal Stem Cell Spheroids with Methacrylated Gelatin Ink for Adipose Tissue Engineering.” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, vol. 31, no. 4, 2020, doi:10.1007/s10856-020-06374-w.
APA
Colle, J., Blondeel, P., De Bruyne, A., Bochar, S., Tytgat, L., Vercruysse, C., … Declercq, H. (2020). Bioprinting predifferentiated adipose-derived mesenchymal stem cell spheroids with methacrylated gelatin ink for adipose tissue engineering. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 31(4). https://doi.org/10.1007/s10856-020-06374-w
Chicago author-date
Colle, Julien, Phillip Blondeel, Axelle De Bruyne, Silke Bochar, Liesbeth Tytgat, Chris Vercruysse, Sandra Van Vlierberghe, Peter Dubruel, and Heidi Declercq. 2020. “Bioprinting Predifferentiated Adipose-Derived Mesenchymal Stem Cell Spheroids with Methacrylated Gelatin Ink for Adipose Tissue Engineering.” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE 31 (4). https://doi.org/10.1007/s10856-020-06374-w.
Chicago author-date (all authors)
Colle, Julien, Phillip Blondeel, Axelle De Bruyne, Silke Bochar, Liesbeth Tytgat, Chris Vercruysse, Sandra Van Vlierberghe, Peter Dubruel, and Heidi Declercq. 2020. “Bioprinting Predifferentiated Adipose-Derived Mesenchymal Stem Cell Spheroids with Methacrylated Gelatin Ink for Adipose Tissue Engineering.” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE 31 (4). doi:10.1007/s10856-020-06374-w.
Vancouver
1.
Colle J, Blondeel P, De Bruyne A, Bochar S, Tytgat L, Vercruysse C, et al. Bioprinting predifferentiated adipose-derived mesenchymal stem cell spheroids with methacrylated gelatin ink for adipose tissue engineering. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE. 2020;31(4).
IEEE
[1]
J. Colle et al., “Bioprinting predifferentiated adipose-derived mesenchymal stem cell spheroids with methacrylated gelatin ink for adipose tissue engineering,” JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, vol. 31, no. 4, 2020.
@article{8666898,
  abstract     = {{The increasing number of mastectomies results in a greater demand for breast reconstruction characterized by simplicity and a low complication profile. Reconstructive surgeons are investigating tissue engineering (TE) strategies to overcome the current surgical drawbacks. 3D bioprinting is the rising technique for the fabrication of large tissue constructs which provides a potential solution for unmet clinical needs in breast reconstruction building on decades of experience in autologous fat grafting, adipose-derived mesenchymal stem cell (ASC) biology and TE. A scaffold was bioprinted using encapsulated ASC spheroids in methacrylated gelatin ink (GelMA). Uniform ASC spheroids with an ideal geometry and diameter for bioprinting were formed, using a high-throughput non-adhesive agarose microwell system. ASC spheroids in adipogenic differentiation medium (ADM) were evaluated through live/dead staining, histology (HE, Oil Red O), TEM and RT-qPCR. Viable spheroids were obtained for up to 14 days post-printing and showed multilocular microvacuoles and successful differentiation toward mature adipocytes shown by gene expression analysis. Moreover, spheroids were able to assemble at random in GelMA, creating a macrotissue. Combining the advantage of microtissues to self-assemble and the controlled organization by bioprinting technologies, these ASC spheroids can be useful as building blocks for the engineering of soft tissue implants.}},
  articleno    = {{36}},
  author       = {{Colle, Julien and Blondeel, Phillip and De Bruyne, Axelle and Bochar, Silke and Tytgat, Liesbeth and Vercruysse, Chris and Van Vlierberghe, Sandra and Dubruel, Peter and Declercq, Heidi}},
  issn         = {{0957-4530}},
  journal      = {{JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE}},
  keywords     = {{DIFFERENTIATION,HYDROGELS,CONSTRUCTS,COMPONENTS,DESIGN}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{15}},
  title        = {{Bioprinting predifferentiated adipose-derived mesenchymal stem cell spheroids with methacrylated gelatin ink for adipose tissue engineering}},
  url          = {{http://doi.org/10.1007/s10856-020-06374-w}},
  volume       = {{31}},
  year         = {{2020}},
}
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