Advanced search
1 file | 1.64 MB Add to list

Isolation of drug-resistant Gallibacterium anatis from calves with unresponsive bronchopneumonia, Belgium

(2020) EMERGING INFECTIOUS DISEASES. 26(4). p.721-730
Author
Organization
Abstract
Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5-7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibactenum genus or even the Pasteurellaceae family, such as aadA23, bla(CARB-8), tet(Y), and qnrDl1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential.
Keywords
BOVINE RESPIRATORY-DISEASE, CONJUGATIVE ELEMENT ICE, ANTIMICROBIAL SUSCEPTIBILITY, PASTEURELLA-HAEMOLYTICA, FEEDLOT CATTLE, IDENTIFICATION, TRACT, MULTOCIDA, SEQUENCE

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.64 MB

Citation

Please use this url to cite or link to this publication:

MLA
Van Driessche, Laura, et al. “Isolation of Drug-Resistant Gallibacterium Anatis from Calves with Unresponsive Bronchopneumonia, Belgium.” EMERGING INFECTIOUS DISEASES, vol. 26, no. 4, 2020, pp. 721–30, doi:10.3201/eid2604.190962.
APA
Van Driessche, L., Vanneste, K., Bogaerts, B., De Keersmaecker, S., Roosens, N., Haesebrouck, F., … Boyen, F. (2020). Isolation of drug-resistant Gallibacterium anatis from calves with unresponsive bronchopneumonia, Belgium. EMERGING INFECTIOUS DISEASES, 26(4), 721–730. https://doi.org/10.3201/eid2604.190962
Chicago author-date
Van Driessche, Laura, Kevin Vanneste, Bert Bogaerts, Sigrid De Keersmaecker, Nancy Roosens, Freddy Haesebrouck, Lieze De Cremer, Piet Deprez, Bart Pardon, and Filip Boyen. 2020. “Isolation of Drug-Resistant Gallibacterium Anatis from Calves with Unresponsive Bronchopneumonia, Belgium.” EMERGING INFECTIOUS DISEASES 26 (4): 721–30. https://doi.org/10.3201/eid2604.190962.
Chicago author-date (all authors)
Van Driessche, Laura, Kevin Vanneste, Bert Bogaerts, Sigrid De Keersmaecker, Nancy Roosens, Freddy Haesebrouck, Lieze De Cremer, Piet Deprez, Bart Pardon, and Filip Boyen. 2020. “Isolation of Drug-Resistant Gallibacterium Anatis from Calves with Unresponsive Bronchopneumonia, Belgium.” EMERGING INFECTIOUS DISEASES 26 (4): 721–730. doi:10.3201/eid2604.190962.
Vancouver
1.
Van Driessche L, Vanneste K, Bogaerts B, De Keersmaecker S, Roosens N, Haesebrouck F, et al. Isolation of drug-resistant Gallibacterium anatis from calves with unresponsive bronchopneumonia, Belgium. EMERGING INFECTIOUS DISEASES. 2020;26(4):721–30.
IEEE
[1]
L. Van Driessche et al., “Isolation of drug-resistant Gallibacterium anatis from calves with unresponsive bronchopneumonia, Belgium,” EMERGING INFECTIOUS DISEASES, vol. 26, no. 4, pp. 721–730, 2020.
@article{8666835,
  abstract     = {{Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5-7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibactenum genus or even the Pasteurellaceae family, such as aadA23, bla(CARB-8), tet(Y), and qnrDl1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential.}},
  author       = {{Van Driessche, Laura and Vanneste, Kevin and Bogaerts, Bert and De Keersmaecker, Sigrid and Roosens, Nancy and Haesebrouck, Freddy and De Cremer, Lieze and Deprez, Piet and Pardon, Bart and Boyen, Filip}},
  issn         = {{1080-6040}},
  journal      = {{EMERGING INFECTIOUS DISEASES}},
  keywords     = {{BOVINE RESPIRATORY-DISEASE,CONJUGATIVE ELEMENT ICE,ANTIMICROBIAL SUSCEPTIBILITY,PASTEURELLA-HAEMOLYTICA,FEEDLOT CATTLE,IDENTIFICATION,TRACT,MULTOCIDA,SEQUENCE}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{721--730}},
  title        = {{Isolation of drug-resistant Gallibacterium anatis from calves with unresponsive bronchopneumonia, Belgium}},
  url          = {{http://dx.doi.org/10.3201/eid2604.190962}},
  volume       = {{26}},
  year         = {{2020}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: