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Influence of blood collection methods and long-term plasma storage on quorum-sensing peptide stability

(2020) ACS OMEGA. 5(26). p.16120-16127
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Abstract
Finding adequate biomarkers for rapid and accurate disease detection, prognosis, and therapy is increasingly important. Quorum-sensing peptides are herein a new emerging group, produced by bacteria, fungi, protozoa, and viruses, with blood being the most straightforward sample type to detect/quantitate them. However, detailed information about suitable blood sample collection methods and storage conditions for measuring these quorum-sensing peptides hampers further clinical research and development. Here, we first tested the time-dependent stability of a set of chemically diverse quorum-sensing peptides, spiked in blood at different temperatures (4, 21, and 37 °C) in four different ethylenediamine tetraacetic acid (EDTA)-containing plasma tubes (with different protein-stabilizing additives) over a period of up to 7.5 h. Next, we determined the storage stability of these quorum-sensing peptides in plasma at different temperatures (4, −35, and −80 °C). UPLC/MS–MS was used to selectively detect and quantify the spiked quorum-sensing peptides. The results of this study indicate that a cost-effective tube, designed for traditional proteomics and stored at 4 °C, is the preferred collection condition when quorum-sensing peptides need to be detected/quantified in human plasma. When the tubes are handled at room temperature (21 °C), a more specialized tube is required. Long-term storage of plasma samples, even under low-temperature conditions (−80 °C), indicates rapid degradation of certain quorum-sensing peptides.
Keywords
PROTEASE INHIBITORS, RISK STRATIFICATION, CHEMICAL SPACE, BIOMARKERS, CLASSIFICATION, CHROMATOGRAPHY, GLUCAGON, TUBES

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MLA
Debunne, Nathan, et al. “Influence of Blood Collection Methods and Long-Term Plasma Storage on Quorum-Sensing Peptide Stability.” ACS OMEGA, vol. 5, no. 26, 2020, pp. 16120–27, doi:10.1021/acsomega.0c01723.
APA
Debunne, N., De Spiegeleer, A., Depuydt, D., Janssens, Y., Descamps, A., Wynendaele, E., & De Spiegeleer, B. (2020). Influence of blood collection methods and long-term plasma storage on quorum-sensing peptide stability. ACS OMEGA, 5(26), 16120–16127. https://doi.org/10.1021/acsomega.0c01723
Chicago author-date
Debunne, Nathan, Anton De Spiegeleer, Dorian Depuydt, Yorick Janssens, Amélie Descamps, Evelien Wynendaele, and Bart De Spiegeleer. 2020. “Influence of Blood Collection Methods and Long-Term Plasma Storage on Quorum-Sensing Peptide Stability.” ACS OMEGA 5 (26): 16120–27. https://doi.org/10.1021/acsomega.0c01723.
Chicago author-date (all authors)
Debunne, Nathan, Anton De Spiegeleer, Dorian Depuydt, Yorick Janssens, Amélie Descamps, Evelien Wynendaele, and Bart De Spiegeleer. 2020. “Influence of Blood Collection Methods and Long-Term Plasma Storage on Quorum-Sensing Peptide Stability.” ACS OMEGA 5 (26): 16120–16127. doi:10.1021/acsomega.0c01723.
Vancouver
1.
Debunne N, De Spiegeleer A, Depuydt D, Janssens Y, Descamps A, Wynendaele E, et al. Influence of blood collection methods and long-term plasma storage on quorum-sensing peptide stability. ACS OMEGA. 2020;5(26):16120–7.
IEEE
[1]
N. Debunne et al., “Influence of blood collection methods and long-term plasma storage on quorum-sensing peptide stability,” ACS OMEGA, vol. 5, no. 26, pp. 16120–16127, 2020.
@article{8666060,
  abstract     = {{Finding adequate biomarkers for rapid and accurate disease detection, prognosis, and therapy is increasingly important. Quorum-sensing peptides are herein a new emerging group, produced by bacteria, fungi, protozoa, and viruses, with blood being the most straightforward sample type to detect/quantitate them. However, detailed information about suitable blood sample collection methods and storage conditions for measuring these quorum-sensing peptides hampers further clinical research and development. Here, we first tested the time-dependent stability of a set of chemically diverse quorum-sensing peptides, spiked in blood at different temperatures (4, 21, and 37 °C) in four different ethylenediamine tetraacetic acid (EDTA)-containing plasma tubes (with different protein-stabilizing additives) over a period of up to 7.5 h. Next, we determined the storage stability of these quorum-sensing peptides in plasma at different temperatures (4, −35, and −80 °C). UPLC/MS–MS was used to selectively detect and quantify the spiked quorum-sensing peptides. The results of this study indicate that a cost-effective tube, designed for traditional proteomics and stored at 4 °C, is the preferred collection condition when quorum-sensing peptides need to be detected/quantified in human plasma. When the tubes are handled at room temperature (21 °C), a more specialized tube is required. Long-term storage of plasma samples, even under low-temperature conditions (−80 °C), indicates rapid degradation of certain quorum-sensing peptides.}},
  author       = {{Debunne, Nathan and De Spiegeleer, Anton and Depuydt, Dorian and Janssens, Yorick and Descamps, Amélie and Wynendaele, Evelien and De Spiegeleer, Bart}},
  issn         = {{2470-1343}},
  journal      = {{ACS OMEGA}},
  keywords     = {{PROTEASE INHIBITORS,RISK STRATIFICATION,CHEMICAL SPACE,BIOMARKERS,CLASSIFICATION,CHROMATOGRAPHY,GLUCAGON,TUBES}},
  language     = {{eng}},
  number       = {{26}},
  pages        = {{16120--16127}},
  title        = {{Influence of blood collection methods and long-term plasma storage on quorum-sensing peptide stability}},
  url          = {{http://dx.doi.org/10.1021/acsomega.0c01723}},
  volume       = {{5}},
  year         = {{2020}},
}

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