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Distinctive growth and transcriptional changes of the diatom Seminavis robusta in response to quorum sensing related compounds

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Abstract
In aquatic habitats, diatoms are frequently found in association with Proteobacteria, many members of which employ cell-to-cell communication via N-acyl homoserine lactones (AHLs). It has been suggested that diatoms could distinguish between beneficial and algicidal bacteria in their surroundings by sensing AHLs. Although some microalgae can interfere with AHL signaling, e.g., by releasing AHL mimics or degrading them, molecular responses to AHLs in microalgae are still unclear. Therefore, we tested the effects of short-chained AHLs, i.e., N-hexanoyl homoserine lactone (C6-HSL), N-3-hydroxyhexanoyl homoserine lactone (OH-C6-HSL), and N-3-oxohexanoyl homoserine lactone (oxo-C6-HSL) and long-chained AHLs, i.e., N-tetradecanoyl homoserine lactone (C14-HSL), N-3-hydroxytetradecanoyl homoserine lactone (OH-C14-HSL), and N-3-oxotetradecanoyl homoserine lactone (oxo-C14-HSL), on growth of the benthic diatom Seminavis robusta. All tested short-chained AHLs did not affect diatom growth, while long-chained AHLs promoted (C14-HSL) or inhibited (OH-C14-HSL and oxo-C14-HSL) growth. To investigate the physiological effects of these long-chained AHLs in more detail, an RNA-seq experiment was performed during which S. robusta was treated with the growth-promoting C14-HSL and the growth-inhibiting oxo-C14-HSL. One tetramic acid was also tested (TA14), a structural rearrangement product of oxo-C14-HSL, which also induced growth inhibition in S. robusta. After 3 days of treatment, analysis revealed that 3,410 genes were differentially expressed in response to at least one of the compounds. In the treatment with the growth-promoting C14-HSL many genes involved in intracellular signaling were upregulated. On the other hand, exposure to growth-inhibiting oxo-C14-HSL and TA14 triggered a switch in lipid metabolism towards increased fatty acid degradation. In addition, oxo-C14-HSL led to downregulation of cell cycle genes, which is in agreement with the stagnation of cell growth in this treatment. Combined, our results indicate that bacterial signaling molecules with high structural similarity induce contrasting physiological responses in S. robusta.
Keywords
Microbiology (medical), Microbiology, N-acyl homoserine lactones, AHLs, tetramic acid, quorum sensing, diatoms, Seminavis robusta, RNA-seq, interkingdom-signaling, DIFFERENTIAL EXPRESSION ANALYSIS, ACYL-HOMOSERINE LACTONES, BACTERIA, PHYTOPLANKTON, MICROPHYTOBENTHOS, COMMUNICATION, MOLECULES, DISCOVERY, DATABASE, SIGNALS

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MLA
Stock genannt Schroer, Frederike, et al. “Distinctive Growth and Transcriptional Changes of the Diatom Seminavis Robusta in Response to Quorum Sensing Related Compounds.” FRONTIERS IN MICROBIOLOGY, vol. 11, 2020, doi:10.3389/fmicb.2020.01240.
APA
Stock genannt Schroer, F., Bilcke, G., De Decker, S., Osuna, C., Van den Berge, K., Vancaester, E., … Vyverman, W. (2020). Distinctive growth and transcriptional changes of the diatom Seminavis robusta in response to quorum sensing related compounds. FRONTIERS IN MICROBIOLOGY, 11. https://doi.org/10.3389/fmicb.2020.01240
Chicago author-date
Stock genannt Schroer, Frederike, Gust Bilcke, Sam De Decker, Cristina Osuna, Koen Van den Berge, Emmelien Vancaester, Lieven De Veylder, Klaas Vandepoele, Sven Mangelinckx, and Wim Vyverman. 2020. “Distinctive Growth and Transcriptional Changes of the Diatom Seminavis Robusta in Response to Quorum Sensing Related Compounds.” FRONTIERS IN MICROBIOLOGY 11. https://doi.org/10.3389/fmicb.2020.01240.
Chicago author-date (all authors)
Stock genannt Schroer, Frederike, Gust Bilcke, Sam De Decker, Cristina Osuna, Koen Van den Berge, Emmelien Vancaester, Lieven De Veylder, Klaas Vandepoele, Sven Mangelinckx, and Wim Vyverman. 2020. “Distinctive Growth and Transcriptional Changes of the Diatom Seminavis Robusta in Response to Quorum Sensing Related Compounds.” FRONTIERS IN MICROBIOLOGY 11. doi:10.3389/fmicb.2020.01240.
Vancouver
1.
Stock genannt Schroer F, Bilcke G, De Decker S, Osuna C, Van den Berge K, Vancaester E, et al. Distinctive growth and transcriptional changes of the diatom Seminavis robusta in response to quorum sensing related compounds. FRONTIERS IN MICROBIOLOGY. 2020;11.
IEEE
[1]
F. Stock genannt Schroer et al., “Distinctive growth and transcriptional changes of the diatom Seminavis robusta in response to quorum sensing related compounds,” FRONTIERS IN MICROBIOLOGY, vol. 11, 2020.
@article{8665207,
  abstract     = {{In aquatic habitats, diatoms are frequently found in association with Proteobacteria, many members of which employ cell-to-cell communication via N-acyl homoserine lactones (AHLs). It has been suggested that diatoms could distinguish between beneficial and algicidal bacteria in their surroundings by sensing AHLs. Although some microalgae can interfere with AHL signaling, e.g., by releasing AHL mimics or degrading them, molecular responses to AHLs in microalgae are still unclear. Therefore, we tested the effects of short-chained AHLs, i.e., N-hexanoyl homoserine lactone (C6-HSL), N-3-hydroxyhexanoyl homoserine lactone (OH-C6-HSL), and N-3-oxohexanoyl homoserine lactone (oxo-C6-HSL) and long-chained AHLs, i.e., N-tetradecanoyl homoserine lactone (C14-HSL), N-3-hydroxytetradecanoyl homoserine lactone (OH-C14-HSL), and N-3-oxotetradecanoyl homoserine lactone (oxo-C14-HSL), on growth of the benthic diatom Seminavis robusta. All tested short-chained AHLs did not affect diatom growth, while long-chained AHLs promoted (C14-HSL) or inhibited (OH-C14-HSL and oxo-C14-HSL) growth. To investigate the physiological effects of these long-chained AHLs in more detail, an RNA-seq experiment was performed during which S. robusta was treated with the growth-promoting C14-HSL and the growth-inhibiting oxo-C14-HSL. One tetramic acid was also tested (TA14), a structural rearrangement product of oxo-C14-HSL, which also induced growth inhibition in S. robusta. After 3 days of treatment, analysis revealed that 3,410 genes were differentially expressed in response to at least one of the compounds. In the treatment with the growth-promoting C14-HSL many genes involved in intracellular signaling were upregulated. On the other hand, exposure to growth-inhibiting oxo-C14-HSL and TA14 triggered a switch in lipid metabolism towards increased fatty acid degradation. In addition, oxo-C14-HSL led to downregulation of cell cycle genes, which is in agreement with the stagnation of cell growth in this treatment. Combined, our results indicate that bacterial signaling molecules with high structural similarity induce contrasting physiological responses in S. robusta.}},
  articleno    = {{1240}},
  author       = {{Stock genannt Schroer, Frederike and Bilcke, Gust and De Decker, Sam and Osuna, Cristina and Van den Berge, Koen and Vancaester, Emmelien and De Veylder, Lieven and Vandepoele, Klaas and Mangelinckx, Sven and Vyverman, Wim}},
  issn         = {{1664-302X}},
  journal      = {{FRONTIERS IN MICROBIOLOGY}},
  keywords     = {{Microbiology (medical),Microbiology,N-acyl homoserine lactones,AHLs,tetramic acid,quorum sensing,diatoms,Seminavis robusta,RNA-seq,interkingdom-signaling,DIFFERENTIAL EXPRESSION ANALYSIS,ACYL-HOMOSERINE LACTONES,BACTERIA,PHYTOPLANKTON,MICROPHYTOBENTHOS,COMMUNICATION,MOLECULES,DISCOVERY,DATABASE,SIGNALS}},
  language     = {{eng}},
  pages        = {{14}},
  title        = {{Distinctive growth and transcriptional changes of the diatom Seminavis robusta in response to quorum sensing related compounds}},
  url          = {{http://dx.doi.org/10.3389/fmicb.2020.01240}},
  volume       = {{11}},
  year         = {{2020}},
}

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