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Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR

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Abstract
There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.
Keywords
Microbiology (medical), Molecular Biology, Microbiology, HOLLOW-FIBER ULTRAFILTRATION, HEPATITIS-A VIRUS, DRINKING-WATER, DIVERSE MICROBES, RECOVERY, OUTBREAK, ENTEROVIRUSES, ADENOVIRUSES, CONSUMPTION, QUALITY

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MLA
Machado-Moreira, Bernardino, et al. “Impact of Beef Extract Used for Sample Concentration on the Detection of Escherichia Coli DNA in Water Samples via QPCR.” JOURNAL OF MICROBIOLOGICAL METHODS, vol. 168, 2020, doi:10.1016/j.mimet.2019.105786.
APA
Machado-Moreira, B., Monteiro, S., Santos, R., Martinez-Murcia, A., Rajkovic, A., Smigic, N., … Burgess, C. M. (2020). Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. JOURNAL OF MICROBIOLOGICAL METHODS, 168. https://doi.org/10.1016/j.mimet.2019.105786
Chicago author-date
Machado-Moreira, Bernardino, Silvia Monteiro, Ricardo Santos, Antonio Martinez-Murcia, Andreja Rajkovic, Nada Smigic, Karl G. Richards, Florence Abram, and Catherine M. Burgess. 2020. “Impact of Beef Extract Used for Sample Concentration on the Detection of Escherichia Coli DNA in Water Samples via QPCR.” JOURNAL OF MICROBIOLOGICAL METHODS 168. https://doi.org/10.1016/j.mimet.2019.105786.
Chicago author-date (all authors)
Machado-Moreira, Bernardino, Silvia Monteiro, Ricardo Santos, Antonio Martinez-Murcia, Andreja Rajkovic, Nada Smigic, Karl G. Richards, Florence Abram, and Catherine M. Burgess. 2020. “Impact of Beef Extract Used for Sample Concentration on the Detection of Escherichia Coli DNA in Water Samples via QPCR.” JOURNAL OF MICROBIOLOGICAL METHODS 168. doi:10.1016/j.mimet.2019.105786.
Vancouver
1.
Machado-Moreira B, Monteiro S, Santos R, Martinez-Murcia A, Rajkovic A, Smigic N, et al. Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. JOURNAL OF MICROBIOLOGICAL METHODS. 2020;168.
IEEE
[1]
B. Machado-Moreira et al., “Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR,” JOURNAL OF MICROBIOLOGICAL METHODS, vol. 168, 2020.
@article{8663872,
  abstract     = {There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.},
  articleno    = {105786},
  author       = {Machado-Moreira, Bernardino and Monteiro, Silvia and Santos, Ricardo and Martinez-Murcia, Antonio and Rajkovic, Andreja and Smigic, Nada and Richards, Karl G. and Abram, Florence and Burgess, Catherine M.},
  issn         = {0167-7012},
  journal      = {JOURNAL OF MICROBIOLOGICAL METHODS},
  keywords     = {Microbiology (medical),Molecular Biology,Microbiology,HOLLOW-FIBER ULTRAFILTRATION,HEPATITIS-A VIRUS,DRINKING-WATER,DIVERSE MICROBES,RECOVERY,OUTBREAK,ENTEROVIRUSES,ADENOVIRUSES,CONSUMPTION,QUALITY},
  language     = {eng},
  pages        = {3},
  title        = {Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR},
  url          = {http://dx.doi.org/10.1016/j.mimet.2019.105786},
  volume       = {168},
  year         = {2020},
}

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