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Evolution of experimental design and research techniques in HIV-1 reservoir studies : a systematic review

(2020) AIDS REVIEWS. 22(1). p.16-24
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Abstract
Although HIV-1 has evolved from a deadly to a chronic disease over the past 20 years, an HIV-1 cure is still lacking due to the presence of persisting cellular viral reservoirs which are spread throughout the body in different anatomical compartments. Hence, the identification and characterization of these HIV-1 reservoirs were the focus of many studies during the past decades. In this review, a systematic literature screening and text mining approach were implemented to assess the evolution in experimental design of these HIV-1 reservoir studies. For this purpose. the online databases PubMed, Web of Science. and ClinicalTrials.gov were consulted and 1768 articles were identified, of which 106 are included in this review. We observed several evolutions that indicate a more structured approach of recent HIV-1 reservoir studies. This includes the use of well-characterized patient cohorts, tissue sampling at several time points and anatomical compartments, the inclusion of patients with different treatment status (on and off antiretroviral therapy), and the implementation of state-of-the-art research techniques such as single genome sequencing. In addition, there is an increased interest and sampling of lymphoid tissues and cerebrospinal fluid together with methods to investigate cellular subsets and HIV-1 sequences. Overall, this review describes an observed shift from detecting and quantifying HIV-1 toward a qualitative in-depth assessment of anatomical reservoirs and cellular subsets playing a role in H1V-1 persistence/latency. These trends coincide with the evolution in focus from controlling HIV-1 replication by currently available antiretroviral therapy toward HIV-1 curative strategies.
Keywords
Pharmacology (medical), Infectious Diseases, General Medicine, HIV-1 reservoir, HIV-1 latency, Tissue compartments, HIV-1 cure, Research methodology, ANTIRETROVIRAL THERAPY, CEREBROSPINAL-FLUID, LYMPHOID-TISSUE, CELLS, DNA, REPLICATION

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MLA
De Scheerder, Marie-Angélique, et al. “Evolution of Experimental Design and Research Techniques in HIV-1 Reservoir Studies : A Systematic Review.” AIDS REVIEWS, vol. 22, no. 1, 2020, pp. 16–24, doi:10.24875/aidsrev.m20000028.
APA
De Scheerder, M.-A., Depelseneer, B., Vandekerckhove, L., & Wim, T. (2020). Evolution of experimental design and research techniques in HIV-1 reservoir studies : a systematic review. AIDS REVIEWS, 22(1), 16–24. https://doi.org/10.24875/aidsrev.m20000028
Chicago author-date
De Scheerder, Marie-Angélique, Bram Depelseneer, Linos Vandekerckhove, and Trypsteen Wim. 2020. “Evolution of Experimental Design and Research Techniques in HIV-1 Reservoir Studies : A Systematic Review.” AIDS REVIEWS 22 (1): 16–24. https://doi.org/10.24875/aidsrev.m20000028.
Chicago author-date (all authors)
De Scheerder, Marie-Angélique, Bram Depelseneer, Linos Vandekerckhove, and Trypsteen Wim. 2020. “Evolution of Experimental Design and Research Techniques in HIV-1 Reservoir Studies : A Systematic Review.” AIDS REVIEWS 22 (1): 16–24. doi:10.24875/aidsrev.m20000028.
Vancouver
1.
De Scheerder M-A, Depelseneer B, Vandekerckhove L, Wim T. Evolution of experimental design and research techniques in HIV-1 reservoir studies : a systematic review. AIDS REVIEWS. 2020;22(1):16–24.
IEEE
[1]
M.-A. De Scheerder, B. Depelseneer, L. Vandekerckhove, and T. Wim, “Evolution of experimental design and research techniques in HIV-1 reservoir studies : a systematic review,” AIDS REVIEWS, vol. 22, no. 1, pp. 16–24, 2020.
@article{8662677,
  abstract     = {{Although HIV-1 has evolved from a deadly to a chronic disease over the past 20 years, an HIV-1 cure is still lacking due to the presence of persisting cellular viral reservoirs which are spread throughout the body in different anatomical compartments. Hence, the identification and characterization of these HIV-1 reservoirs were the focus of many studies during the past decades. In this review, a systematic literature screening and text mining approach were implemented to assess the evolution in experimental design of these HIV-1 reservoir studies. For this purpose. the online databases PubMed, Web of Science. and ClinicalTrials.gov were consulted and 1768 articles were identified, of which 106 are included in this review. We observed several evolutions that indicate a more structured approach of recent HIV-1 reservoir studies. This includes the use of well-characterized patient cohorts, tissue sampling at several time points and anatomical compartments, the inclusion of patients with different treatment status (on and off antiretroviral therapy), and the implementation of state-of-the-art research techniques such as single genome sequencing. In addition, there is an increased interest and sampling of lymphoid tissues and cerebrospinal fluid together with methods to investigate cellular subsets and HIV-1 sequences. Overall, this review describes an observed shift from detecting and quantifying HIV-1 toward a qualitative in-depth assessment of anatomical reservoirs and cellular subsets playing a role in H1V-1 persistence/latency. These trends coincide with the evolution in focus from controlling HIV-1 replication by currently available antiretroviral therapy toward HIV-1 curative strategies.}},
  author       = {{De Scheerder, Marie-Angélique and Depelseneer, Bram and Vandekerckhove, Linos and Wim, Trypsteen}},
  issn         = {{1139-6121}},
  journal      = {{AIDS REVIEWS}},
  keywords     = {{Pharmacology (medical),Infectious Diseases,General Medicine,HIV-1 reservoir,HIV-1 latency,Tissue compartments,HIV-1 cure,Research methodology,ANTIRETROVIRAL THERAPY,CEREBROSPINAL-FLUID,LYMPHOID-TISSUE,CELLS,DNA,REPLICATION}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{16--24}},
  title        = {{Evolution of experimental design and research techniques in HIV-1 reservoir studies : a systematic review}},
  url          = {{http://dx.doi.org/10.24875/aidsrev.m20000028}},
  volume       = {{22}},
  year         = {{2020}},
}

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