Advanced search
1 file | 1.42 MB Add to list

Beta-glucan's varying structure characteristics modulate survival and immune-related genes expression from Vibrio harveyi-infected Artemia franciscana in gnotobiotic conditions

Biao Han (UGent) , Kartik Baruah, Viet Dung Nguyen (UGent) , David L. Williams, Bert Devriendt (UGent) , Eric Cox (UGent) and Peter Bossier (UGent)
(2020) FISH & SHELLFISH IMMUNOLOGY. 102. p.307-315
Author
Organization
Abstract
beta-Glucans have long been used as an immunostimulant in aquaculture. However, the relationship of its structure to its immunomodulatory properties are poorly understood. In this study, the particle size and chemical structure of beta-glucans extracted from wild-type strain of baker's yeast (Saccharomyces cerevisiae) and its null-mutant yeasts Gas1 were characterised. Using Sigma beta-glucan as a reference, the immunomodulatory properties of these polysaccharides in the germ-free Anemia franciscana model system in the presence of Vibrio harveyi bacterial challenge were investigated. The survival of the A. franciscana nauplii, upon challenge with V. harveyi, was significantly higher in all three glucan-treated groups compared to the control. The glucan Gas1 with a lower degree of branching and shorter side chain length had the most prominent V. harveyi-protective effects. The particle size did not affect the nauplii survival when challenged with V. harveyi. Results also showed that the salutary effect of the tested glucans was associated with the upregulation of innate immune genes such as lipopolysaccharide and beta-1,3-glucan-binding protein (lgbp), high mobility group box protein (hmgb), and prophenoloxidase (proPO). Interestingly, the up-regulation of superoxidase dismutase (sod) and glutathione-stransferase (gst) was only observed in Gas1 treated group, indicating that Gas1 could function to induce higher reactive oxygen species and stronger immunomodulatory function in A. franciscana, and therefore higher survival rate. The expression of heat shock protein 70 (hsp70), peroxinectin (pm), and down syndrome cell adhesion molecule (dscam) remain unaltered in response to glucan treatment. Taken together, this study provides insights into the structure-function relationship of beta-glucan and the results confirmed that beta-glucan can be an effective immunostimulant in aquaculture, especially the Gas1 glucan.
Keywords
Aquatic Science, Environmental Chemistry, General Medicine, beta-Glucan, Immune-related genes, Structure-function relationship, Artemia franciscana, Gnotobiotic, Vibrio harveyi, CELL-WALL MUTANTS, MOLECULAR-CLONING, SHRIMP, DISEASE, IMMUNOSTIMULANT, PHAGOCYTOSIS, ALTERNATIVES, RECOGNITION, PERFORMANCE, CAMPBELLII

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.42 MB

Citation

Please use this url to cite or link to this publication:

MLA
Han, Biao, et al. “Beta-Glucan’s Varying Structure Characteristics Modulate Survival and Immune-Related Genes Expression from Vibrio Harveyi-Infected Artemia Franciscana in Gnotobiotic Conditions.” FISH & SHELLFISH IMMUNOLOGY, vol. 102, 2020, pp. 307–15, doi:10.1016/j.fsi.2020.04.062.
APA
Han, B., Baruah, K., Nguyen, V. D., Williams, D. L., Devriendt, B., Cox, E., & Bossier, P. (2020). Beta-glucan’s varying structure characteristics modulate survival and immune-related genes expression from Vibrio harveyi-infected Artemia franciscana in gnotobiotic conditions. FISH & SHELLFISH IMMUNOLOGY, 102, 307–315. https://doi.org/10.1016/j.fsi.2020.04.062
Chicago author-date
Han, Biao, Kartik Baruah, Viet Dung Nguyen, David L. Williams, Bert Devriendt, Eric Cox, and Peter Bossier. 2020. “Beta-Glucan’s Varying Structure Characteristics Modulate Survival and Immune-Related Genes Expression from Vibrio Harveyi-Infected Artemia Franciscana in Gnotobiotic Conditions.” FISH & SHELLFISH IMMUNOLOGY 102: 307–15. https://doi.org/10.1016/j.fsi.2020.04.062.
Chicago author-date (all authors)
Han, Biao, Kartik Baruah, Viet Dung Nguyen, David L. Williams, Bert Devriendt, Eric Cox, and Peter Bossier. 2020. “Beta-Glucan’s Varying Structure Characteristics Modulate Survival and Immune-Related Genes Expression from Vibrio Harveyi-Infected Artemia Franciscana in Gnotobiotic Conditions.” FISH & SHELLFISH IMMUNOLOGY 102: 307–315. doi:10.1016/j.fsi.2020.04.062.
Vancouver
1.
Han B, Baruah K, Nguyen VD, Williams DL, Devriendt B, Cox E, et al. Beta-glucan’s varying structure characteristics modulate survival and immune-related genes expression from Vibrio harveyi-infected Artemia franciscana in gnotobiotic conditions. FISH & SHELLFISH IMMUNOLOGY. 2020;102:307–15.
IEEE
[1]
B. Han et al., “Beta-glucan’s varying structure characteristics modulate survival and immune-related genes expression from Vibrio harveyi-infected Artemia franciscana in gnotobiotic conditions,” FISH & SHELLFISH IMMUNOLOGY, vol. 102, pp. 307–315, 2020.
@article{8662542,
  abstract     = {{beta-Glucans have long been used as an immunostimulant in aquaculture. However, the relationship of its structure to its immunomodulatory properties are poorly understood. In this study, the particle size and chemical structure of beta-glucans extracted from wild-type strain of baker's yeast (Saccharomyces cerevisiae) and its null-mutant yeasts Gas1 were characterised. Using Sigma beta-glucan as a reference, the immunomodulatory properties of these polysaccharides in the germ-free Anemia franciscana model system in the presence of Vibrio harveyi bacterial challenge were investigated. The survival of the A. franciscana nauplii, upon challenge with V. harveyi, was significantly higher in all three glucan-treated groups compared to the control. The glucan Gas1 with a lower degree of branching and shorter side chain length had the most prominent V. harveyi-protective effects. The particle size did not affect the nauplii survival when challenged with V. harveyi. Results also showed that the salutary effect of the tested glucans was associated with the upregulation of innate immune genes such as lipopolysaccharide and beta-1,3-glucan-binding protein (lgbp), high mobility group box protein (hmgb), and prophenoloxidase (proPO). Interestingly, the up-regulation of superoxidase dismutase (sod) and glutathione-stransferase (gst) was only observed in Gas1 treated group, indicating that Gas1 could function to induce higher reactive oxygen species and stronger immunomodulatory function in A. franciscana, and therefore higher survival rate. The expression of heat shock protein 70 (hsp70), peroxinectin (pm), and down syndrome cell adhesion molecule (dscam) remain unaltered in response to glucan treatment. Taken together, this study provides insights into the structure-function relationship of beta-glucan and the results confirmed that beta-glucan can be an effective immunostimulant in aquaculture, especially the Gas1 glucan.}},
  author       = {{Han, Biao and Baruah, Kartik and Nguyen, Viet Dung and Williams, David L. and Devriendt, Bert and Cox, Eric and Bossier, Peter}},
  issn         = {{1050-4648}},
  journal      = {{FISH & SHELLFISH IMMUNOLOGY}},
  keywords     = {{Aquatic Science,Environmental Chemistry,General Medicine,beta-Glucan,Immune-related genes,Structure-function relationship,Artemia franciscana,Gnotobiotic,Vibrio harveyi,CELL-WALL MUTANTS,MOLECULAR-CLONING,SHRIMP,DISEASE,IMMUNOSTIMULANT,PHAGOCYTOSIS,ALTERNATIVES,RECOGNITION,PERFORMANCE,CAMPBELLII}},
  language     = {{eng}},
  pages        = {{307--315}},
  title        = {{Beta-glucan's varying structure characteristics modulate survival and immune-related genes expression from Vibrio harveyi-infected Artemia franciscana in gnotobiotic conditions}},
  url          = {{http://dx.doi.org/10.1016/j.fsi.2020.04.062}},
  volume       = {{102}},
  year         = {{2020}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: