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Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection

Cedric Bosteels (UGent) , Katrijn Neyt (UGent) , Manon Vanheerswynghels (UGent) , Mary van Helden (UGent) , Dorine Sichien (UGent) , Nincy Debeuf (UGent) , Sofie De Prijck (UGent) , Victor Bosteels (UGent) , Niels Vandamme (UGent) , Liesbet Martens (UGent) , et al.
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Abstract
The phenotypic and functional dichotomy between IRF8+ type 1 and IRF4+ type 2 conventional dendritic cells (cDC1s and cDC2s, respectively) is well accepted; it is unknown how robust this dichotomy is under inflammatory conditions, when additionally monocyte-derived cells (MCs) become competent antigen-presenting cells (APCs). Using single-cell technologies in models of respiratory viral infection, we found that lung cDC2s acquired expression of the Fc receptor CD64 shared with MCs and of IRF8 shared with cDC1s. These inflammatory cDC2s (inf-cDC2s) were superior in inducing CD4+ T helper (Th) cell polarization while simultaneously presenting antigen to CD8+ T cells. When carefully separated from inf-cDC2s, MCs lacked APC function. Inf-cDC2s matured in response to cell-intrinsic Toll-like receptor and type 1 interferon receptor signaling, upregulated an IRF8-dependent maturation module, and acquired antigens via convalescent serum and Fc receptors. Because hybrid inf-cDC2s are easily confused with monocyte-derived cells, their existence could explain why APC functions have been attributed to MCs.
Keywords
Immunology, Immunology and Allergy, Infectious Diseases

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MLA
Bosteels, Cedric, et al. “Inflammatory Type 2 CDCs Acquire Features of CDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.” IMMUNITY, 2020.
APA
Bosteels, C., Neyt, K., Vanheerswynghels, M., van Helden, M., Sichien, D., Debeuf, N., … Lambrecht, B. (2020). Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection. IMMUNITY.
Chicago author-date
Bosteels, Cedric, Katrijn Neyt, Manon Vanheerswynghels, Mary van Helden, Dorine Sichien, Nincy Debeuf, Sofie De Prijck, et al. 2020. “Inflammatory Type 2 CDCs Acquire Features of CDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.” IMMUNITY.
Chicago author-date (all authors)
Bosteels, Cedric, Katrijn Neyt, Manon Vanheerswynghels, Mary van Helden, Dorine Sichien, Nincy Debeuf, Sofie De Prijck, Victor Bosteels, Niels Vandamme, Liesbet Martens, Yvan Saeys, Els Louagie, Manon Lesage, David L. Williams, Shiau-Choot Tang, Johannes U. Mayer, Franca Ronchese, Charlotte Scott, Hamida Hammad, Martin Guilliams, and Bart Lambrecht. 2020. “Inflammatory Type 2 CDCs Acquire Features of CDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.” IMMUNITY.
Vancouver
1.
Bosteels C, Neyt K, Vanheerswynghels M, van Helden M, Sichien D, Debeuf N, et al. Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection. IMMUNITY. 2020;
IEEE
[1]
C. Bosteels et al., “Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection,” IMMUNITY, 2020.
@article{8662161,
  abstract     = {The phenotypic and functional dichotomy between IRF8+ type 1 and IRF4+ type 2 conventional dendritic cells (cDC1s and cDC2s, respectively) is well accepted; it is unknown how robust this dichotomy is under inflammatory conditions, when additionally monocyte-derived cells (MCs) become competent antigen-presenting cells (APCs). Using single-cell technologies in models of respiratory viral infection, we found that lung cDC2s acquired expression of the Fc receptor CD64 shared with MCs and of IRF8 shared with cDC1s. These inflammatory cDC2s (inf-cDC2s) were superior in inducing CD4+ T helper (Th) cell polarization while simultaneously presenting antigen to CD8+ T cells. When carefully separated from inf-cDC2s, MCs lacked APC function. Inf-cDC2s matured in response to cell-intrinsic Toll-like receptor and type 1 interferon receptor signaling, upregulated an IRF8-dependent maturation module, and acquired antigens via convalescent serum and Fc receptors. Because hybrid inf-cDC2s are easily confused with monocyte-derived cells, their existence could explain why APC functions have been attributed to MCs.},
  author       = {Bosteels, Cedric and Neyt, Katrijn and Vanheerswynghels, Manon and van Helden, Mary and Sichien, Dorine and Debeuf, Nincy and De Prijck, Sofie and Bosteels, Victor and Vandamme, Niels and Martens, Liesbet and Saeys, Yvan and Louagie, Els and Lesage, Manon and Williams, David L. and Tang, Shiau-Choot and Mayer, Johannes U. and Ronchese, Franca and Scott, Charlotte and Hammad, Hamida and Guilliams, Martin and Lambrecht, Bart},
  issn         = {1074-7613},
  journal      = {IMMUNITY},
  keywords     = {Immunology,Immunology and Allergy,Infectious Diseases},
  language     = {eng},
  title        = {Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection},
  url          = {http://dx.doi.org/10.1016/j.immuni.2020.04.005},
  year         = {2020},
}

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