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The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis

Stephanie Smith, Shanshuo Zhu (UGent) , Lisa Joos (UGent) , Ianto Roberts (UGent) , Natalia Nikonorova (UGent) , Lam Dai Vu (UGent) , Elisabeth Stes (UGent) , Hyunwoo Cho, Antoine Larrieu, Wei Xuan (UGent) , et al.
(2020) MOLECULAR & CELLULAR PROTEOMICS. 19(8). p.1248-1262
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Abstract
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.
Keywords
Analytical Chemistry, Biochemistry, Molecular Biology, ENHANCES DROUGHT TOLERANCE, AUXIN RESPONSE, NUCLEAR-LOCALIZATION, OSMOTIC-STRESS, REGULATE ROOT, RECEPTOR, WATER, GENE, EXPRESSION, KINASES

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Citation

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MLA
Smith, Stephanie, et al. “The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.” MOLECULAR & CELLULAR PROTEOMICS, vol. 19, no. 8, 2020, pp. 1248–62, doi:10.1074/mcp.ra119.001826.
APA
Smith, S., Zhu, S., Joos, L., Roberts, I., Nikonorova, N., Vu, L. D., … De Smet, I. (2020). The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. MOLECULAR & CELLULAR PROTEOMICS, 19(8), 1248–1262. https://doi.org/10.1074/mcp.ra119.001826
Chicago author-date
Smith, Stephanie, Shanshuo Zhu, Lisa Joos, Ianto Roberts, Natalia Nikonorova, Lam Dai Vu, Elisabeth Stes, et al. 2020. “The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.” MOLECULAR & CELLULAR PROTEOMICS 19 (8): 1248–62. https://doi.org/10.1074/mcp.ra119.001826.
Chicago author-date (all authors)
Smith, Stephanie, Shanshuo Zhu, Lisa Joos, Ianto Roberts, Natalia Nikonorova, Lam Dai Vu, Elisabeth Stes, Hyunwoo Cho, Antoine Larrieu, Wei Xuan, Benjamin Goodall, Brigitte Van De Cotte, Jessic Marie Waite, Adeline Rigal, Sigurd R. Harborough, Geert Persiau, Steffen Vanneste, Gwendolyn K. Kirschner, Elien Vandermarliere, Lennart Martens, Yvonne Stahl, Dominique Audenaert, Jiri Friml, Georg Felix, Ruediger Simon, Malcolm Bennett, Anthony Bishopp, Geert De Jaeger, Karin Ljung, Stefan Kepinski, Stephanie Robert, Jennifer Nemhauser, Ildoo Hwang, Kris Gevaert, Tom Beeckman, and Ive De Smet. 2020. “The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.” MOLECULAR & CELLULAR PROTEOMICS 19 (8): 1248–1262. doi:10.1074/mcp.ra119.001826.
Vancouver
1.
Smith S, Zhu S, Joos L, Roberts I, Nikonorova N, Vu LD, et al. The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. MOLECULAR & CELLULAR PROTEOMICS. 2020;19(8):1248–62.
IEEE
[1]
S. Smith et al., “The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis,” MOLECULAR & CELLULAR PROTEOMICS, vol. 19, no. 8, pp. 1248–1262, 2020.
@article{8662093,
  abstract     = {Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.},
  author       = {Smith, Stephanie and Zhu, Shanshuo and Joos, Lisa and Roberts, Ianto and Nikonorova, Natalia and Vu, Lam Dai and Stes, Elisabeth and Cho, Hyunwoo and Larrieu, Antoine and Xuan, Wei and Goodall, Benjamin and Van De Cotte, Brigitte and Waite, Jessic Marie and Rigal, Adeline and Harborough, Sigurd R. and Persiau, Geert and Vanneste, Steffen and Kirschner, Gwendolyn K. and Vandermarliere, Elien and Martens, Lennart and Stahl, Yvonne and Audenaert, Dominique and Friml, Jiri and Felix, Georg and Simon, Ruediger and Bennett, Malcolm and Bishopp, Anthony and De Jaeger, Geert and Ljung, Karin and Kepinski, Stefan and Robert, Stephanie and Nemhauser, Jennifer and Hwang, Ildoo and Gevaert, Kris and Beeckman, Tom and De Smet, Ive},
  issn         = {1535-9476},
  journal      = {MOLECULAR & CELLULAR PROTEOMICS},
  keywords     = {Analytical Chemistry,Biochemistry,Molecular Biology,ENHANCES DROUGHT TOLERANCE,AUXIN RESPONSE,NUCLEAR-LOCALIZATION,OSMOTIC-STRESS,REGULATE ROOT,RECEPTOR,WATER,GENE,EXPRESSION,KINASES},
  language     = {eng},
  number       = {8},
  pages        = {1248--1262},
  title        = {The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis},
  url          = {http://dx.doi.org/10.1074/mcp.ra119.001826},
  volume       = {19},
  year         = {2020},
}

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