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Toxicokinetics and toxicodynamics of the fentanyl homologs cyclopropanoyl-1-benzyl-4 '-fluoro-4-anilinopiperidine and furanoyl-1-benzyl-4-anilinopiperidine

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Abstract
The two fentanyl homologs cyclopropanoyl-1-benzyl-4 '-fluoro-4-anilinopiperidine (4F-Cy-BAP) and furanoyl-1-benzyl-4-anilinopiperidine (Fu-BAP) have recently been seized as new psychoactive substances (NPS) on the drugs of abuse market. As their toxicokinetic and toxicodynamic characteristics are completely unknown, this study focused on elucidating their in vitro metabolic stability in pooled human liver S9 fraction (pHLS9), their qualitative in vitro (pHLS9), and in vivo (zebrafish larvae) metabolism, and their in vitro isozyme mapping using recombinant expressed isoenzymes. Their maximum-tolerated concentration (MTC) in zebrafish larvae was studied from 0.01 to 100 mu M. Their mu-opioid receptor (MOR) activity was analyzed in engineered human embryonic kidney (HEK) 293 T cells. In total, seven phase I and one phase II metabolites of 4F-Cy-BAP and 15 phase I and four phase II metabolites of Fu-BAP were tentatively identified by means of liquid chromatography high-resolution tandem mass spectrometry, with the majority detected in zebrafish larvae. N-Dealkylation, N-deacylation, hydroxylation, and N-oxidation were the most abundant metabolic reactions and the corresponding metabolites are expected to be promising analytical targets for toxicological analysis. Isozyme mapping revealed the main involvement of CYP3A4 in the phase I metabolism of 4F-Cy-BAP and in terms of Fu-BAP additionally CYP2D6. Therefore, drug-drug interactions by CYP3A4 inhibition may cause elevated drug levels and unwanted adverse effects. MTC experiments revealed malformations and changes in the behavior of larvae after exposure to 100 mu M Fu-BAP. Both substances were only able to produce a weak activation of MOR and although toxic effects based on MOR activation seem unlikely, activity at other receptors cannot be excluded.
Keywords
PLASMA-PROTEIN BINDING, INTRINSIC CLEARANCE, METABOLIC STABILITY, HALF-LIFE, DISCOVERY, SERIES, DRUGS, In vitro and in vivo metabolism, Metabolic stability, LC-HRMS, MS, Zebrafish larvae, In vitro mu-opioid receptor activity

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MLA
Gampfer, Tanja M., et al. “Toxicokinetics and Toxicodynamics of the Fentanyl Homologs Cyclopropanoyl-1-Benzyl-4 ’-Fluoro-4-Anilinopiperidine and Furanoyl-1-Benzyl-4-Anilinopiperidine.” ARCHIVES OF TOXICOLOGY, Springer Heidelberg, 2020.
APA
Gampfer, T. M., Wagmann, L., Park, Y. M., Cannaert, A., Herrmann, J., Fischmann, S., … Meyer, M. R. (2020). Toxicokinetics and toxicodynamics of the fentanyl homologs cyclopropanoyl-1-benzyl-4 ’-fluoro-4-anilinopiperidine and furanoyl-1-benzyl-4-anilinopiperidine. ARCHIVES OF TOXICOLOGY.
Chicago author-date
Gampfer, Tanja M., Lea Wagmann, Yu Mi Park, Annelies Cannaert, Jennifer Herrmann, Svenja Fischmann, Folker Westphal, Rolf Mueller, Christophe Stove, and Markus R. Meyer. 2020. “Toxicokinetics and Toxicodynamics of the Fentanyl Homologs Cyclopropanoyl-1-Benzyl-4 ’-Fluoro-4-Anilinopiperidine and Furanoyl-1-Benzyl-4-Anilinopiperidine.” ARCHIVES OF TOXICOLOGY.
Chicago author-date (all authors)
Gampfer, Tanja M., Lea Wagmann, Yu Mi Park, Annelies Cannaert, Jennifer Herrmann, Svenja Fischmann, Folker Westphal, Rolf Mueller, Christophe Stove, and Markus R. Meyer. 2020. “Toxicokinetics and Toxicodynamics of the Fentanyl Homologs Cyclopropanoyl-1-Benzyl-4 ’-Fluoro-4-Anilinopiperidine and Furanoyl-1-Benzyl-4-Anilinopiperidine.” ARCHIVES OF TOXICOLOGY.
Vancouver
1.
Gampfer TM, Wagmann L, Park YM, Cannaert A, Herrmann J, Fischmann S, et al. Toxicokinetics and toxicodynamics of the fentanyl homologs cyclopropanoyl-1-benzyl-4 ’-fluoro-4-anilinopiperidine and furanoyl-1-benzyl-4-anilinopiperidine. ARCHIVES OF TOXICOLOGY. 2020;
IEEE
[1]
T. M. Gampfer et al., “Toxicokinetics and toxicodynamics of the fentanyl homologs cyclopropanoyl-1-benzyl-4 ’-fluoro-4-anilinopiperidine and furanoyl-1-benzyl-4-anilinopiperidine,” ARCHIVES OF TOXICOLOGY, 2020.
@article{8662056,
  abstract     = {The two fentanyl homologs cyclopropanoyl-1-benzyl-4 '-fluoro-4-anilinopiperidine (4F-Cy-BAP) and furanoyl-1-benzyl-4-anilinopiperidine (Fu-BAP) have recently been seized as new psychoactive substances (NPS) on the drugs of abuse market. As their toxicokinetic and toxicodynamic characteristics are completely unknown, this study focused on elucidating their in vitro metabolic stability in pooled human liver S9 fraction (pHLS9), their qualitative in vitro (pHLS9), and in vivo (zebrafish larvae) metabolism, and their in vitro isozyme mapping using recombinant expressed isoenzymes. Their maximum-tolerated concentration (MTC) in zebrafish larvae was studied from 0.01 to 100 mu M. Their mu-opioid receptor (MOR) activity was analyzed in engineered human embryonic kidney (HEK) 293 T cells. In total, seven phase I and one phase II metabolites of 4F-Cy-BAP and 15 phase I and four phase II metabolites of Fu-BAP were tentatively identified by means of liquid chromatography high-resolution tandem mass spectrometry, with the majority detected in zebrafish larvae. N-Dealkylation, N-deacylation, hydroxylation, and N-oxidation were the most abundant metabolic reactions and the corresponding metabolites are expected to be promising analytical targets for toxicological analysis. Isozyme mapping revealed the main involvement of CYP3A4 in the phase I metabolism of 4F-Cy-BAP and in terms of Fu-BAP additionally CYP2D6. Therefore, drug-drug interactions by CYP3A4 inhibition may cause elevated drug levels and unwanted adverse effects. MTC experiments revealed malformations and changes in the behavior of larvae after exposure to 100 mu M Fu-BAP. Both substances were only able to produce a weak activation of MOR and although toxic effects based on MOR activation seem unlikely, activity at other receptors cannot be excluded.},
  author       = {Gampfer, Tanja M. and Wagmann, Lea and Park, Yu Mi and Cannaert, Annelies and Herrmann, Jennifer and Fischmann, Svenja and Westphal, Folker and Mueller, Rolf and Stove, Christophe and Meyer, Markus R.},
  issn         = {0340-5761},
  journal      = {ARCHIVES OF TOXICOLOGY},
  keywords     = {PLASMA-PROTEIN BINDING,INTRINSIC CLEARANCE,METABOLIC STABILITY,HALF-LIFE,DISCOVERY,SERIES,DRUGS,In vitro and in vivo metabolism,Metabolic stability,LC-HRMS,MS,Zebrafish larvae,In vitro mu-opioid receptor activity},
  language     = {eng},
  pages        = {17},
  publisher    = {Springer Heidelberg},
  title        = {Toxicokinetics and toxicodynamics of the fentanyl homologs cyclopropanoyl-1-benzyl-4 '-fluoro-4-anilinopiperidine and furanoyl-1-benzyl-4-anilinopiperidine},
  url          = {http://dx.doi.org/10.1007/s00204-020-02726-1},
  year         = {2020},
}

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