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Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola

Sofie Lodens (UGent) , Sophie Roelants (UGent) , Goedele Luyten (UGent) , Robin Geys (UGent) , Pieter Coussement (UGent) , Sofie De Maeseneire (UGent) and Wim Soetaert (UGent)
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Abstract
Starmerella bombicola very efficiently produces the secondary metabolites sophorolipids (SLs). Their biosynthesis is not-growth associated and highly upregulated in the stationary phase. Despite high industrial and academic interest, the underlying regulation of SL biosynthesis remains unknown. In this paper, potential regulation of SL biosynthesis through the telomere positioning effect (TPE) was investigated, as the SL gene cluster is located adjacent to a telomere. An additional copy of this gene cluster was introduced elsewhere in the genome to investigate if this results in a decoy of regulation. Indeed, for the new strain, the onset of SL production was shifted to the exponential phase. This result was confirmed by RT-qPCR analysis. The TPE effect was further investigated by developing and applying a suitable reporter system for this non-conventional yeast, enabling non-biased comparison of gene expression between the subtelomeric CYP52M1- and the URA3 locus. This was done with a constitutive endogenous promotor (pGAPD) and one of the endogenous promotors of the SL biosynthetic gene cluster (pCYP52M1). A clear positioning effect was observed for both promotors with significantly higher GFP expression levels at the URA3 locus. No clear GFP upregulation was observed in the stationary phase for any of the new strains.
Keywords
Applied Microbiology and Biotechnology, Microbiology, General Medicine, secondary metabolites, biosurfactants, Starmerella bombicola, regulation, telomere positioning effect, sophorolipids, SECONDARY METABOLISM, LIPID PRODUCTION, GENE-EXPRESSION, YEAST, LANDSCAPE, HETEROCHROMATIN, BIOSURFACTANTS, TRANSFORMATION, REPORTER, CLUSTERS

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MLA
Lodens, Sofie, et al. “Unraveling the Regulation of Sophorolipid Biosynthesis in Starmerella Bombicola.” FEMS YEAST RESEARCH, vol. 20, no. 3, 2020, doi:10.1093/femsyr/foaa021.
APA
Lodens, S., Roelants, S., Luyten, G., Geys, R., Coussement, P., De Maeseneire, S., & Soetaert, W. (2020). Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola. FEMS YEAST RESEARCH, 20(3). https://doi.org/10.1093/femsyr/foaa021
Chicago author-date
Lodens, Sofie, Sophie Roelants, Goedele Luyten, Robin Geys, Pieter Coussement, Sofie De Maeseneire, and Wim Soetaert. 2020. “Unraveling the Regulation of Sophorolipid Biosynthesis in Starmerella Bombicola.” FEMS YEAST RESEARCH 20 (3). https://doi.org/10.1093/femsyr/foaa021.
Chicago author-date (all authors)
Lodens, Sofie, Sophie Roelants, Goedele Luyten, Robin Geys, Pieter Coussement, Sofie De Maeseneire, and Wim Soetaert. 2020. “Unraveling the Regulation of Sophorolipid Biosynthesis in Starmerella Bombicola.” FEMS YEAST RESEARCH 20 (3). doi:10.1093/femsyr/foaa021.
Vancouver
1.
Lodens S, Roelants S, Luyten G, Geys R, Coussement P, De Maeseneire S, et al. Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola. FEMS YEAST RESEARCH. 2020;20(3).
IEEE
[1]
S. Lodens et al., “Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola,” FEMS YEAST RESEARCH, vol. 20, no. 3, 2020.
@article{8661860,
  abstract     = {{Starmerella bombicola very efficiently produces the secondary metabolites sophorolipids (SLs). Their biosynthesis is not-growth associated and highly upregulated in the stationary phase. Despite high industrial and academic interest, the underlying regulation of SL biosynthesis remains unknown. In this paper, potential regulation of SL biosynthesis through the telomere positioning effect (TPE) was investigated, as the SL gene cluster is located adjacent to a telomere. An additional copy of this gene cluster was introduced elsewhere in the genome to investigate if this results in a decoy of regulation. Indeed, for the new strain, the onset of SL production was shifted to the exponential phase. This result was confirmed by RT-qPCR analysis. The TPE effect was further investigated by developing and applying a suitable reporter system for this non-conventional yeast, enabling non-biased comparison of gene expression between the subtelomeric CYP52M1- and the URA3 locus. This was done with a constitutive endogenous promotor (pGAPD) and one of the endogenous promotors of the SL biosynthetic gene cluster (pCYP52M1). A clear positioning effect was observed for both promotors with significantly higher GFP expression levels at the URA3 locus. No clear GFP upregulation was observed in the stationary phase for any of the new strains.}},
  articleno    = {{foaa021}},
  author       = {{Lodens, Sofie and Roelants, Sophie and Luyten, Goedele and Geys, Robin and Coussement, Pieter and De Maeseneire, Sofie and Soetaert, Wim}},
  issn         = {{1567-1356}},
  journal      = {{FEMS YEAST RESEARCH}},
  keywords     = {{Applied Microbiology and Biotechnology,Microbiology,General Medicine,secondary metabolites,biosurfactants,Starmerella bombicola,regulation,telomere positioning effect,sophorolipids,SECONDARY METABOLISM,LIPID PRODUCTION,GENE-EXPRESSION,YEAST,LANDSCAPE,HETEROCHROMATIN,BIOSURFACTANTS,TRANSFORMATION,REPORTER,CLUSTERS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{11}},
  title        = {{Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola}},
  url          = {{http://dx.doi.org/10.1093/femsyr/foaa021}},
  volume       = {{20}},
  year         = {{2020}},
}

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