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Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells

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Abstract
Tissue engineering aims to structurally and functionally regenerate damaged tissues, which requires the formation of new blood vessels that supply oxygen and nutrients by the process of angiogenesis. Stem cells are a promising tool in regenerative medicine due to their combined differentiation and paracrine angiogenic capacities. The study of their proangiogenic properties and associated potential for tissue regeneration requires complex in vivo models comprising all steps of the angiogenic process. The highly vascularized extraembryonic chorioallantoic membrane (CAM) of fertilized chicken eggs offers a simple, easy accessible, and cheap angiogenic screening tool compared to other animal models. Although the CAM assay was initially primarily performed for evaluation of tumor growth and metastasis, stem cell studies using this model are increasing. In this review, a detailed summary of angiogenic observations of different mesenchymal, cardiac, and endothelial stem cell types and derivatives in the CAM model is presented. Moreover, we focus on the variation in experimental setup, including the benefits and limitations of in ovo and ex ovo protocols, diverse biological and synthetic scaffolds, imaging techniques, and outcome measures of neovascularization. Finally, advantages and disadvantages of the CAM assay as a model for angiogenesis in tissue engineering in comparison with alternative in vivo animal models are described.
Keywords
Biochemistry, Bioengineering, Biomaterials, Biomedical Engineering

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Please use this url to cite or link to this publication:

MLA
Merckx, Greet, et al. “Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells.” Tissue Engineering Part B: Reviews, 2020.
APA
Merckx, G., Tay, H., Lo Monaco, M., van Zandvoort, M., De Spiegelaere, W., Lambrichts, I., & Bronckaers, A. (2020). Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells. Tissue Engineering Part B: Reviews.
Chicago author-date
Merckx, Greet, Hanna Tay, Melissa Lo Monaco, Marc van Zandvoort, Ward De Spiegelaere, Ivo Lambrichts, and Annelies Bronckaers. 2020. “Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells.” Tissue Engineering Part B: Reviews.
Chicago author-date (all authors)
Merckx, Greet, Hanna Tay, Melissa Lo Monaco, Marc van Zandvoort, Ward De Spiegelaere, Ivo Lambrichts, and Annelies Bronckaers. 2020. “Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells.” Tissue Engineering Part B: Reviews.
Vancouver
1.
Merckx G, Tay H, Lo Monaco M, van Zandvoort M, De Spiegelaere W, Lambrichts I, et al. Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells. Tissue Engineering Part B: Reviews. 2020;
IEEE
[1]
G. Merckx et al., “Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells,” Tissue Engineering Part B: Reviews, 2020.
@article{8661644,
  abstract     = {Tissue engineering aims to structurally and functionally regenerate damaged tissues, which requires the formation of new blood vessels that supply oxygen and nutrients by the process of angiogenesis. Stem cells are a promising tool in regenerative medicine due to their combined differentiation and paracrine angiogenic capacities. The study of their proangiogenic properties and associated potential for tissue regeneration requires complex in vivo models comprising all steps of the angiogenic process. The highly vascularized extraembryonic chorioallantoic membrane (CAM) of fertilized chicken eggs offers a simple, easy accessible, and cheap angiogenic screening tool compared to other animal models. Although the CAM assay was initially primarily performed for evaluation of tumor growth and metastasis, stem cell studies using this model are increasing. In this review, a detailed summary of angiogenic observations of different mesenchymal, cardiac, and endothelial stem cell types and derivatives in the CAM model is presented. Moreover, we focus on the variation in experimental setup, including the benefits and limitations of in ovo and ex ovo protocols, diverse biological and synthetic scaffolds, imaging techniques, and outcome measures of neovascularization. Finally, advantages and disadvantages of the CAM assay as a model for angiogenesis in tissue engineering in comparison with alternative in vivo animal models are described.},
  author       = {Merckx, Greet and Tay, Hanna and Lo Monaco, Melissa and van Zandvoort, Marc and De Spiegelaere, Ward and Lambrichts, Ivo and Bronckaers, Annelies},
  issn         = {1937-3368},
  journal      = {Tissue Engineering Part B: Reviews},
  keywords     = {Biochemistry,Bioengineering,Biomaterials,Biomedical Engineering},
  language     = {eng},
  title        = {Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells},
  url          = {http://dx.doi.org/10.1089/ten.teb.2020.0048},
  year         = {2020},
}

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