Advanced search
Add to list

Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B

(2011) JOURNAL OF GENERAL VIROLOGY. 92(9). p.2181-2190
Author
Organization
Abstract
Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Junin virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections.
Keywords
Virology, TRACT-BINDING-PROTEIN, LYMPHOCYTIC CHORIOMENINGITIS VIRUS, HNRNP A/B PROTEINS, MESSENGER-RNA, SINDBIS-VIRUS, K INTERACTS, REPLICATION, INHIBITION, RELOCALIZATION, TRANSLATION

Citation

Please use this url to cite or link to this publication:

MLA
Maeto, Cynthia A., et al. “Differential Effect of Acute and Persistent Junín Virus Infections on the Nucleo-Cytoplasmic Trafficking and Expression of Heterogeneous Nuclear Ribonucleoproteins Type A and B.” JOURNAL OF GENERAL VIROLOGY, vol. 92, no. 9, 2011, pp. 2181–90, doi:10.1099/vir.0.030163-0.
APA
Maeto, C. A., Knott, M. E., Linero, F., Ellenberg, P. C., Scolaro, L. A., & Castilla, V. (2011). Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B. JOURNAL OF GENERAL VIROLOGY, 92(9), 2181–2190. https://doi.org/10.1099/vir.0.030163-0
Chicago author-date
Maeto, Cynthia A., María E. Knott, Florencia Linero, Paula C. Ellenberg, Luis A. Scolaro, and Viviana Castilla. 2011. “Differential Effect of Acute and Persistent Junín Virus Infections on the Nucleo-Cytoplasmic Trafficking and Expression of Heterogeneous Nuclear Ribonucleoproteins Type A and B.” JOURNAL OF GENERAL VIROLOGY 92 (9): 2181–90. https://doi.org/10.1099/vir.0.030163-0.
Chicago author-date (all authors)
Maeto, Cynthia A., María E. Knott, Florencia Linero, Paula C. Ellenberg, Luis A. Scolaro, and Viviana Castilla. 2011. “Differential Effect of Acute and Persistent Junín Virus Infections on the Nucleo-Cytoplasmic Trafficking and Expression of Heterogeneous Nuclear Ribonucleoproteins Type A and B.” JOURNAL OF GENERAL VIROLOGY 92 (9): 2181–2190. doi:10.1099/vir.0.030163-0.
Vancouver
1.
Maeto CA, Knott ME, Linero F, Ellenberg PC, Scolaro LA, Castilla V. Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B. JOURNAL OF GENERAL VIROLOGY. 2011;92(9):2181–90.
IEEE
[1]
C. A. Maeto, M. E. Knott, F. Linero, P. C. Ellenberg, L. A. Scolaro, and V. Castilla, “Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B,” JOURNAL OF GENERAL VIROLOGY, vol. 92, no. 9, pp. 2181–2190, 2011.
@article{8661493,
  abstract     = {{Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Junin virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections.}},
  author       = {{Maeto, Cynthia A. and Knott, María E. and Linero, Florencia and Ellenberg, Paula C. and Scolaro, Luis A. and Castilla, Viviana}},
  issn         = {{0022-1317}},
  journal      = {{JOURNAL OF GENERAL VIROLOGY}},
  keywords     = {{Virology,TRACT-BINDING-PROTEIN,LYMPHOCYTIC CHORIOMENINGITIS VIRUS,HNRNP A/B PROTEINS,MESSENGER-RNA,SINDBIS-VIRUS,K INTERACTS,REPLICATION,INHIBITION,RELOCALIZATION,TRANSLATION}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{2181--2190}},
  title        = {{Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B}},
  url          = {{http://dx.doi.org/10.1099/vir.0.030163-0}},
  volume       = {{92}},
  year         = {{2011}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: