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Junín virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation

(2011) JOURNAL OF GENERAL VIROLOGY. 92(12). p.2889-2899
Author
Organization
Abstract
Stress granules (SGs) are ephemeral cytoplasmic aggregates containing stalled translation preinitiation complexes involved in mRNA storage and triage during the cellular stress response. SG formation is triggered by the phosphorylation of the alpha subunit of elF2 (eIF2 alpha), which provokes a dramatic blockage of protein translation. Our results demonstrate that acute infection of Vero cells with the arenavirus Junin (JUNV), aetiological agent of Argentine haemorrhagic fever, does not induce the formation of SGs. Moreover, JUNV negatively modulates SG formation in infected cells stressed with arsenite, and this inhibition correlates with low levels of elF2 alpha phosphorylation. Transient expression of JUNV nucleoprotein (N) or the glycoprotein precursor (GPC), but not of the matrix protein (Z), inhibits SG formation in a similar manner, comparable to infectious virus. Expression of N and GPC also impaired elF2a phosphorylation triggered by arsenite. A moderate inhibition of SG formation was also observed when DTT and thapsigargin were employed as stress inducers. In contrast, no inhibition was observed when infected cells were treated with hippuristanol, a translational inhibitor and inducer of SGs that bypasses the requirement for elF2 alpha phosphorylation. Finally, we analysed SG formation in persistently JUN V-infected cells, where N and GPC are virtually absent and truncated N products are expressed abundantly. We found that persistently infected cells show a quite normal response to arsenite, with SG formation comparable to that of uninfected cells. This suggests that the presence of GPC and/or N is crucial to control the stress response upon JUNV infection of Vero cells.
Keywords
Virology, TRANSLATION INITIATION, WEST-NILE, REPLICATION, PROTEIN, RNA, ARENAVIRUSES, EIF2-ALPHA, EUKARYOTES, EXPRESSION, SURVIVAL

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MLA
Linero, Florencia, et al. “Junín Virus Infection Impairs Stress-Granule Formation in Vero Cells Treated with Arsenite via Inhibition of EIF2α Phosphorylation.” JOURNAL OF GENERAL VIROLOGY, vol. 92, no. 12, 2011, pp. 2889–99, doi:10.1099/vir.0.033407-0.
APA
Linero, F., Thomas, M. G., Boccaccio, G. L., & Scolaro, L. A. (2011). Junín virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation. JOURNAL OF GENERAL VIROLOGY, 92(12), 2889–2899. https://doi.org/10.1099/vir.0.033407-0
Chicago author-date
Linero, Florencia, María G. Thomas, Graciela L. Boccaccio, and Luis A. Scolaro. 2011. “Junín Virus Infection Impairs Stress-Granule Formation in Vero Cells Treated with Arsenite via Inhibition of EIF2α Phosphorylation.” JOURNAL OF GENERAL VIROLOGY 92 (12): 2889–99. https://doi.org/10.1099/vir.0.033407-0.
Chicago author-date (all authors)
Linero, Florencia, María G. Thomas, Graciela L. Boccaccio, and Luis A. Scolaro. 2011. “Junín Virus Infection Impairs Stress-Granule Formation in Vero Cells Treated with Arsenite via Inhibition of EIF2α Phosphorylation.” JOURNAL OF GENERAL VIROLOGY 92 (12): 2889–2899. doi:10.1099/vir.0.033407-0.
Vancouver
1.
Linero F, Thomas MG, Boccaccio GL, Scolaro LA. Junín virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation. JOURNAL OF GENERAL VIROLOGY. 2011;92(12):2889–99.
IEEE
[1]
F. Linero, M. G. Thomas, G. L. Boccaccio, and L. A. Scolaro, “Junín virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation,” JOURNAL OF GENERAL VIROLOGY, vol. 92, no. 12, pp. 2889–2899, 2011.
@article{8661492,
  abstract     = {{Stress granules (SGs) are ephemeral cytoplasmic aggregates containing stalled translation preinitiation complexes involved in mRNA storage and triage during the cellular stress response. SG formation is triggered by the phosphorylation of the alpha subunit of elF2 (eIF2 alpha), which provokes a dramatic blockage of protein translation. Our results demonstrate that acute infection of Vero cells with the arenavirus Junin (JUNV), aetiological agent of Argentine haemorrhagic fever, does not induce the formation of SGs. Moreover, JUNV negatively modulates SG formation in infected cells stressed with arsenite, and this inhibition correlates with low levels of elF2 alpha phosphorylation. Transient expression of JUNV nucleoprotein (N) or the glycoprotein precursor (GPC), but not of the matrix protein (Z), inhibits SG formation in a similar manner, comparable to infectious virus. Expression of N and GPC also impaired elF2a phosphorylation triggered by arsenite. A moderate inhibition of SG formation was also observed when DTT and thapsigargin were employed as stress inducers. In contrast, no inhibition was observed when infected cells were treated with hippuristanol, a translational inhibitor and inducer of SGs that bypasses the requirement for elF2 alpha phosphorylation. Finally, we analysed SG formation in persistently JUN V-infected cells, where N and GPC are virtually absent and truncated N products are expressed abundantly. We found that persistently infected cells show a quite normal response to arsenite, with SG formation comparable to that of uninfected cells. This suggests that the presence of GPC and/or N is crucial to control the stress response upon JUNV infection of Vero cells.}},
  author       = {{Linero, Florencia and Thomas, María G. and Boccaccio, Graciela L. and Scolaro, Luis A.}},
  issn         = {{0022-1317}},
  journal      = {{JOURNAL OF GENERAL VIROLOGY}},
  keywords     = {{Virology,TRANSLATION INITIATION,WEST-NILE,REPLICATION,PROTEIN,RNA,ARENAVIRUSES,EIF2-ALPHA,EUKARYOTES,EXPRESSION,SURVIVAL}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{2889--2899}},
  title        = {{Junín virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation}},
  url          = {{http://dx.doi.org/10.1099/vir.0.033407-0}},
  volume       = {{92}},
  year         = {{2011}},
}

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