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New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen

(2020) JOURNAL OF EXPERIMENTAL BOTANY. 71(8). p.2451-2463
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Abstract
Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilization. Mechanistically, one of the best-studied SI systems is that of Papaver rhoeas (poppy). This utilizes two S-determinants: stigma-expressed PrsS and pollen-expressed PrpS. Interaction of cognate PrpS–PrsS triggers a signalling network, causing rapid growth arrest and programmed cell death (PCD) in incompatible pollen. We previously demonstrated that transgenic Arabidopsis thaliana pollen expressing PrpS–green fluorescent protein (GFP) can respond to Papaver PrsS with remarkably similar responses to those observed in incompatible Papaver pollen. Here we describe recent advances using these transgenic plants combined with genetically encoded fluorescent probes to monitor SI-induced cellular alterations, including cytosolic calcium, pH, the actin cytoskeleton, clathrin-mediated endocytosis (CME), and the vacuole. This approach has allowed us to study the SI response in depth, using multiparameter live-cell imaging approaches that were not possible in Papaver. This lays the foundations for new opportunities to elucidate key mechanisms involved in SI. Here we establish that CME is disrupted in self-incompatible pollen. Moreover, we reveal new detailed information about F-actin remodelling in pollen tubes after SI.
Keywords
Plant Science, Physiology, Actin, actin-binding proteins (ABPs), calcium, endocytosis, fluorescent probes, programmed cell death (PCD), pH, pollen tube growth, live-cell imaging, self-incompatibility (SI), ACTIN-DEPOLYMERIZING FACTOR, PROGRAMMED CELL-DEATH, SOMATIC CYTOKINESIS, BINDING PROTEINS, TIP GROWTH, TUBES, CA2+, RHOEAS, TPLATE, ORGANIZATION

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MLA
Wang, Ludi, et al. “New Opportunities and Insights into Papaver Self-Incompatibility by Imaging Engineered Arabidopsis Pollen.” JOURNAL OF EXPERIMENTAL BOTANY, vol. 71, no. 8, 2020, pp. 2451–63, doi:10.1093/jxb/eraa092.
APA
Wang, L., Muñoz Triviño, M., Lin, Z., Carli, J., Eaves, D. J., Van Damme, D., … Bosch, M. (2020). New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen. JOURNAL OF EXPERIMENTAL BOTANY, 71(8), 2451–2463. https://doi.org/10.1093/jxb/eraa092
Chicago author-date
Wang, Ludi, Marina Muñoz Triviño, Zongcheng Lin, José Carli, Deborah J Eaves, Daniël Van Damme, Moritz Nowack, Vernonica E Franklin-Tong, and Maurice Bosch. 2020. “New Opportunities and Insights into Papaver Self-Incompatibility by Imaging Engineered Arabidopsis Pollen.” JOURNAL OF EXPERIMENTAL BOTANY 71 (8): 2451–63. https://doi.org/10.1093/jxb/eraa092.
Chicago author-date (all authors)
Wang, Ludi, Marina Muñoz Triviño, Zongcheng Lin, José Carli, Deborah J Eaves, Daniël Van Damme, Moritz Nowack, Vernonica E Franklin-Tong, and Maurice Bosch. 2020. “New Opportunities and Insights into Papaver Self-Incompatibility by Imaging Engineered Arabidopsis Pollen.” JOURNAL OF EXPERIMENTAL BOTANY 71 (8): 2451–2463. doi:10.1093/jxb/eraa092.
Vancouver
1.
Wang L, Muñoz Triviño M, Lin Z, Carli J, Eaves DJ, Van Damme D, et al. New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen. JOURNAL OF EXPERIMENTAL BOTANY. 2020;71(8):2451–63.
IEEE
[1]
L. Wang et al., “New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen,” JOURNAL OF EXPERIMENTAL BOTANY, vol. 71, no. 8, pp. 2451–2463, 2020.
@article{8660696,
  abstract     = {{Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilization. Mechanistically, one of the best-studied SI systems is that of Papaver rhoeas (poppy). This utilizes two S-determinants: stigma-expressed PrsS and pollen-expressed PrpS. Interaction of cognate PrpS–PrsS triggers a signalling network, causing rapid growth arrest and programmed cell death (PCD) in incompatible pollen. We previously demonstrated that transgenic Arabidopsis thaliana pollen expressing PrpS–green fluorescent protein (GFP) can respond to Papaver PrsS with remarkably similar responses to those observed in incompatible Papaver pollen. Here we describe recent advances using these transgenic plants combined with genetically encoded fluorescent probes to monitor SI-induced cellular alterations, including cytosolic calcium, pH, the actin cytoskeleton, clathrin-mediated endocytosis (CME), and the vacuole. This approach has allowed us to study the SI response in depth, using multiparameter live-cell imaging approaches that were not possible in Papaver. This lays the foundations for new opportunities to elucidate key mechanisms involved in SI. Here we establish that CME is disrupted in self-incompatible pollen. Moreover, we reveal new detailed information about F-actin remodelling in pollen tubes after SI.}},
  author       = {{Wang, Ludi and Muñoz Triviño, Marina and Lin, Zongcheng and Carli, José and Eaves, Deborah J and Van Damme, Daniël and Nowack, Moritz and Franklin-Tong, Vernonica E and Bosch, Maurice}},
  issn         = {{0022-0957}},
  journal      = {{JOURNAL OF EXPERIMENTAL BOTANY}},
  keywords     = {{Plant Science,Physiology,Actin,actin-binding proteins (ABPs),calcium,endocytosis,fluorescent probes,programmed cell death (PCD),pH,pollen tube growth,live-cell imaging,self-incompatibility (SI),ACTIN-DEPOLYMERIZING FACTOR,PROGRAMMED CELL-DEATH,SOMATIC CYTOKINESIS,BINDING PROTEINS,TIP GROWTH,TUBES,CA2+,RHOEAS,TPLATE,ORGANIZATION}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{2451--2463}},
  title        = {{New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen}},
  url          = {{http://dx.doi.org/10.1093/jxb/eraa092}},
  volume       = {{71}},
  year         = {{2020}},
}

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