High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits
- Author
- Jie Wang (UGent) , Evelien Mylle (UGent) , Alexander Johnson, Nienke Besbrugge, Geert De Jaeger (UGent) , Jiří Friml, Roman Pleskot (UGent) and Daniël Van Damme (UGent)
- Organization
- Abstract
- The TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and two plant-specific subunits, AtEH1/Pan1 and AtEH2/Pan1, although cytoplasmic proteins are not associated with the hexameric subcomplex in the cytoplasm. To investigate the dynamic assembly of the octameric TPC at the plasma membrane (PM), we performed state-of-the-art dual-color live cell imaging at physiological and lowered temperatures. Lowering the temperature slowed down endocytosis, thereby enhancing the temporal resolution of the differential recruitment of endocytic components. Under both normal and lowered temperature conditions, the core TPC subunit TPLATE and the AtEH/Pan1 proteins exhibited simultaneous recruitment at the PM. These results, together with co-localization analysis of different TPC subunits, allow us to conclude that TPC in plant cells is not recruited to the PM sequentially but as an octameric complex.
- Keywords
- Plant Science, Genetics, Physiology, CLATHRIN-MEDIATED ENDOCYTOSIS, COATED VESICLE FORMATION, SOMATIC CYTOKINESIS, CELLULOSE SYNTHASE, ARABIDOPSIS, DYNAMICS, ADAPTER, PROTEIN, AP2, TEMPERATURE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8660071
- MLA
- Wang, Jie, et al. “High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.” PLANT PHYSIOLOGY, vol. 183, no. 3, 2020, pp. 986–97, doi:10.1104/pp.20.00178.
- APA
- Wang, J., Mylle, E., Johnson, A., Besbrugge, N., De Jaeger, G., Friml, J., … Van Damme, D. (2020). High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. PLANT PHYSIOLOGY, 183(3), 986–997. https://doi.org/10.1104/pp.20.00178
- Chicago author-date
- Wang, Jie, Evelien Mylle, Alexander Johnson, Nienke Besbrugge, Geert De Jaeger, Jiří Friml, Roman Pleskot, and Daniël Van Damme. 2020. “High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.” PLANT PHYSIOLOGY 183 (3): 986–97. https://doi.org/10.1104/pp.20.00178.
- Chicago author-date (all authors)
- Wang, Jie, Evelien Mylle, Alexander Johnson, Nienke Besbrugge, Geert De Jaeger, Jiří Friml, Roman Pleskot, and Daniël Van Damme. 2020. “High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.” PLANT PHYSIOLOGY 183 (3): 986–997. doi:10.1104/pp.20.00178.
- Vancouver
- 1.Wang J, Mylle E, Johnson A, Besbrugge N, De Jaeger G, Friml J, et al. High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. PLANT PHYSIOLOGY. 2020;183(3):986–97.
- IEEE
- [1]J. Wang et al., “High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits,” PLANT PHYSIOLOGY, vol. 183, no. 3, pp. 986–997, 2020.
@article{8660071,
abstract = {{The TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and two plant-specific subunits, AtEH1/Pan1 and AtEH2/Pan1, although cytoplasmic proteins are not associated with the hexameric subcomplex in the cytoplasm. To investigate the dynamic assembly of the octameric TPC at the plasma membrane (PM), we performed state-of-the-art dual-color live cell imaging at physiological and lowered temperatures. Lowering the temperature slowed down endocytosis, thereby enhancing the temporal resolution of the differential recruitment of endocytic components. Under both normal and lowered temperature conditions, the core TPC subunit TPLATE and the AtEH/Pan1 proteins exhibited simultaneous recruitment at the PM. These results, together with co-localization analysis of different TPC subunits, allow us to conclude that TPC in plant cells is not recruited to the PM sequentially but as an octameric complex.}},
author = {{Wang, Jie and Mylle, Evelien and Johnson, Alexander and Besbrugge, Nienke and De Jaeger, Geert and Friml, Jiří and Pleskot, Roman and Van Damme, Daniël}},
issn = {{0032-0889}},
journal = {{PLANT PHYSIOLOGY}},
keywords = {{Plant Science,Genetics,Physiology,CLATHRIN-MEDIATED ENDOCYTOSIS,COATED VESICLE FORMATION,SOMATIC CYTOKINESIS,CELLULOSE SYNTHASE,ARABIDOPSIS,DYNAMICS,ADAPTER,PROTEIN,AP2,TEMPERATURE}},
language = {{eng}},
number = {{3}},
pages = {{986--997}},
title = {{High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits}},
url = {{http://doi.org/10.1104/pp.20.00178}},
volume = {{183}},
year = {{2020}},
}
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